A5039895112- Enzyme Structure and Function
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Mass Spectrometry Techniques and Applications
- RNA modifications and cancer
- Advanced NMR Techniques and Applications
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Spectroscopy and Quantum Chemical Studies
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA regulation and disease
- Computational Drug Discovery Methods
- Photoreceptor and optogenetics research
- Cholinesterase and Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Molecular spectroscopy and chirality
- Cancer-related gene regulation
- Material Dynamics and Properties
- Microbial Community Ecology and Physiology
- NMR spectroscopy and applications
Institut Laue-Langevin
2010-2025
Institut de Biologie Structurale
2015-2024
CEA Grenoble
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
Université Grenoble Alpes
2015-2024
Direction de la Recherche Fondamentale
2022-2024
Université Joseph Fourier
2006-2014
Max Planck Institute of Biochemistry
2010
Biotechnology Institute
2010
In 2012, preliminary guidelines were published addressing sample quality, data acquisition and reduction, presentation of scattering validation, modelling for biomolecular small-angle (SAS) experiments. Biomolecular SAS has since continued to grow authors have increasingly adopted the guidelines. parallel, integrative/hybrid determination structures is a rapidly growing field that expanding scope structural biology. For contribute maximally this field, it essential ensure open access...
Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; structure, dynamics, properties of resulting complexes. All MPs tested date form water-soluble complexes APols, biochemical stability is in general greatly improved compared detergent solutions. The functionality ligand-binding APol-trapped reviewed,...
Abstract Hydration water is the natural matrix of biological macromolecules and essential for their activity in cells. The coupling between protein dynamics has been intensively studied, yet it remains controversial. Here we combine perdeuteration, neutron scattering molecular simulations to explore nature hydration motions at temperatures 200 300 K, across so-called dynamical transition, intrinsically disordered human tau globular maltose binding protein. Quasi-elastic broadening fitted...
The genome of measles virus is encapsidated by multiple copies the nucleoprotein (N), forming helical nucleocapsids molecular mass approaching 150 Megadalton. intrinsically disordered C-terminal domain N (N TAIL ) essential for transcription and replication via interaction with phosphoprotein P viral polymerase complex. recognition element (MoRE) that binds situated 90 amino acids from folded RNA-binding CORE N, raising questions about functional role this chain. Here we report first in situ...
The dynamical coupling between proteins and their hydration water is important for the understanding of macromolecular function in a cellular context. In case membrane proteins, environment heterogeneous, composed lipids water, might be more complex than extensively studied soluble proteins. Here, we examine biological membrane, purple (PM), its by combination elastic incoherent neutron scattering, specific deuteration, molecular dynamics simulations. Examining completely deuterated PM,...
Abstract Post-transcriptional mechanisms play a predominant role in the control of microRNA (miRNA) production. Recognition terminal loop precursor miRNAs by RNA-binding proteins (RBPs) influences their processing; however, mechanistic basis for how levels individual or subsets are regulated is mostly unexplored. We previously showed that hnRNP A1, an RBP implicated many aspects RNA processing, acts as auxiliary factor promotes Microprocessor-mediated processing pri-mir-18a. Here, using...
Intracellular water dynamics in Haloarcula marismortui , an extremely halophilic organism originally isolated from the Dead Sea, was studied by neutron scattering. The centrifuged cell pellets examined means of two spectrometers, IN6 and IN16, sensitive to motions with time scales 10 ps 1 ns, respectively. From data, a translational diffusion constant 1.3 × −5 cm 2 s −1 determined at 285 K. This value is close that found previously for other cells bulk water, as well 3.5 M NaCl solution...
Multidomain proteins containing intrinsically disordered linkers exhibit large-scale dynamic modes that play key roles in a multitude of molecular recognition and signaling processes. Here, we determine the conformational space sampled by multidomain splicing factor U2AF65 using complementary nuclear magnetic resonance spectroscopy small-angle scattering data. Available degrees freedom are initially stochastically experimental data then used to delineate potential energy landscape terms...
The scattering of neutrons can be used to provide information on the structure and dynamics biological systems multiple length time scales. Pursuant a National Science Foundation-funded workshop in February 2018, recent developments this field are reviewed here, as well future prospects that expected given advances sources, instrumentation computational power methods. Crystallography, solution scattering, dynamics, membranes, labeling imaging examined. For extraction maximum information,...
An integrated picture of hydration shell dynamics and its coupling to functional macromolecular motions is proposed from studies on a soluble protein, membrane protein in natural lipid environment, the intracellular environment bacteria red blood cells. Water multimolar salt solutions was also examined, context very slow water component previously discovered cytoplasm extreme halophilic archaea. The data were obtained neutron scattering by using deuterium labelling focus different parts...
A novel type of nonionic amphipols for handling membrane proteins in detergent-free aqueous solutions has been obtained through free-radical homo-telomerization an acrylamide-based monomer comprising a C(11) alkyl chain and two glucose moieties, using thiol as transfer reagent. By controlling the thiol/monomer ratio, number-average molecular weight polymers was varied from 8 to 63 kDa. Homopolymeric were found be highly soluble water self-organize, within large concentration range, into...
Multi-domain proteins play critical roles in fine-tuning essential processes cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. (RBPs) represent an important class of multi-domain proteins, which regulate expression recognizing linear structured sequence motifs. Here, we employ segmental perdeuteration the three recognition motif (RRM) RBP TIA-1 using...
We investigate laccase-mediated detoxification of aflatoxins, fungal carcinogenic food contaminants. Our experimental comparison between two aflatoxins with similar structures (AFB1 and AFG2) shows significant differences in detoxification. A multi-scale modeling approach (Docking, Molecular Dynamics, Density Functional Theory) identifies the highly substrate-specific changes required to improve laccase detoxifying performance. employ a large-scale density functional theory-based approach,...
There is increasing interest in the contribution of methyl groups to overall dynamics measured by neutron scattering experiments proteins. In particular an inflection observed atomic mean square displacements as a function temperature on high resolution spectrometers (approximately 1 microeV) was explained onset group rotations. By specifically labeling non-methyl-containing side-chain native protein system, purple membrane, and performing measurements, we here provide direct experimental...
Abstract Meiotic recombination is initiated by the formation of DNA double‐strand breaks (DSBs), essential for fertility and genetic diversity. In mouse, DSBs are formed catalytic TOPOVIL complex consisting SPO11 TOPOVIBL. To preserve genome integrity, activity finely controlled several meiotic factors including REC114, MEI4, IHO1, but underlying mechanism poorly understood. Here, we report that mouse REC114 forms homodimers, it associates with MEI4 as a 2:1 heterotrimer further dimerizes,...