A5028205065- Enzyme Structure and Function
- Microstructure and Mechanical Properties of Steels
- Protein Structure and Dynamics
- Nuclear Physics and Applications
- Hydrogen embrittlement and corrosion behaviors in metals
- Hydrogels: synthesis, properties, applications
- Metallurgy and Material Forming
- X-ray Diffraction in Crystallography
- melanin and skin pigmentation
- DNA and Nucleic Acid Chemistry
- Metal and Thin Film Mechanics
- Microstructure and mechanical properties
- Meat and Animal Product Quality
- Magnetic Properties and Applications
- Connexins and lens biology
- Metal Alloys Wear and Properties
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- Biochemical effects in animals
- Glass properties and applications
- Proteins in Food Systems
- Advanced Materials Characterization Techniques
Kyoto University
2016-2025
Osaka University
1986-2024
Nippon Steel (Japan)
2011-2023
Sony (Taiwan)
2022
Kanazawa University
2022
Nippon Steel Chemical and Material (Japan)
2022
Kyoto University Research Reactor Institute
2009-2021
Materials Science & Engineering
2018-2021
Tokyo Metropolitan University
2013-2019
Nippon Steel (Germany)
2013-2018
In 2012, preliminary guidelines were published addressing sample quality, data acquisition and reduction, presentation of scattering validation, modelling for biomolecular small-angle (SAS) experiments. Biomolecular SAS has since continued to grow authors have increasingly adopted the guidelines. parallel, integrative/hybrid determination structures is a rapidly growing field that expanding scope structural biology. For contribute maximally this field, it essential ensure open access...
Abstract Background Neuroinflammation involves the activation of glial cells in neurodegenerative diseases such as Alzheimer’s disease (AD). Plasmalogens (Pls) are glycerophospholipids constituting cellular membranes and play significant roles membrane fluidity processes vesicular fusion signal transduction. Methods In this study preventive effects Pls on systemic lipopolysaccharide (LPS)-induced neuroinflammation were investigated using immunohistochemistry, real-time PCR methods analysis...
Nucleosomes are dynamic entities that repositioned along DNA by chromatin remodeling processes. A nucleosome the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms intermediate "overlapping dinucleosome." Here, we report crystal structure of overlapping dinucleosome, in which two nucleosomes associated, at 3.14-angstrom resolution. In dinucleosome structure, unusual "hexasome" nucleosome, composed histone hexamer lacking one H2A-H2B dimer from conventional...
In order to make clear the influence of matrix microstructures on age-hardening behavior in copper containing steels, precipitation and phase transformation particles during isothermal aging were investigated by differential scanning calorimetry (DSC), transmission electron microscopy (TEM) atom-probe field ion (AP-FIM). ferrite, it was found from DSC analysis TEM observations that clusters, bcc 9R structures exist prior formation stable fcc phase, main structure a maximum hardness specimen...
In Fe–Pd alloys containing about 30 at% palladium, fcc-fct thermoelastic transformation exists, and the fct martensite phase is further transformed non-thermoelastically to bct phase. The morphology structure of martensitic have been extensively studied by means X-ray diffractometry, optical electron microscopy. change in surface relief associated with was investigated detail using single crystals which were made Bridgman method. concentration dependence their Ms temperatures determined...
Abstract The nucleation event of amyloid fibrils is one the most crucial processes that dictate timing and rate pathology diseases; however, information regarding how protein molecules associate to produce fibril nuclei currently limited. In order explore this issue in more detail, we performed time-resolved small angle X-ray scattering (SAXS) measurements on insulin fibrillation, combination with additional multidirectional analyses thioflavin T fluorescence, FTIR spectroscopy, light...
Abstract Nuclear import receptors (NIRs) not only transport RNA-binding proteins (RBPs) but also modify phase transitions of RBPs by recognizing nuclear localization signals (NLSs). Toxic arginine-rich poly-dipeptides from C9orf72 interact with NIRs and cause nucleocytoplasmic deficit. However, the molecular basis for toxicity toward function as modifiers remains unidentified. Here we show that impede ability to RBPs. Isothermal titration calorimetry size-exclusion chromatography revealed...
Polyvinylalcohol (PVA) hydrogel cross-linked by gamma irradiation was assessed as a possible vitreous substitute. From series of experiments, rise intraocular pressure and inflammatory changes in the cavity after operation were observed some cases. Crab-eating macaques used for this experiment. PVA gels injected into vitrectomy followed clinically opthalmoscopy, tonometry, fundus photography, electroretinogram (ERG), chemotaxis, flare cell meter. Histopathologic examination light electron...
Two cDNA clones encoding polyphenol oxidases were isolated from a library constructed log-phase suspension culture of Phytolacca americana (pokeweed) producing betalains. The exhibit 93 and 86% sequence identity at the nucleotide deduced amino acid levels, respectively. Both contain two copper-binding domains characterized by histidine-rich regions, which are found ubiquitously in all oxidases/tyrosinases, putative third histidine-rich, region, is common to plant oxidases. One sequences...
The mechanism of thermoelastic fcc-fct martensite transformation Fe–Pd alloys containing about 30 at%Pd was studied by optical and transmission electron microscopy. It found that the not accompanied with internal twins in beginning transformation, but they were introduced during growth process fct which degree tetragonality increased. In fcc austenite phase, remarkable tweed contrast consisting {011}/〈0\bar11〉 striations accompanying diffuse streaks perpendicular to them observed, lamellae...
In the search for novel peptides that inhibit angiotensin I-converting enzyme (ACE), porcine skeletal troponin was hydrolyzed with pepsin, and products were subjected to various types of chromatography isolate active peptides. Glu-Lys-Glu-Arg-Glu-Arg-Gln (EKERERQ) Lys-Arg-Gln-Lys-Tyr-Asp-Ile (KRQKYDI) identified as peptides, their 50% inhibitory concentrations found be 552.5 26.2 microM, respectively. These are ACE activity KRQKYDI strongest among previously reported troponin-originated...
The same coarse austenite (γ) grains as those before austenitizing emerge when a martensite or bainite steel with is reheated to an region at slow reheating rate. This called abnormal ferrite (α) transformation γ grain memory. In this paper, α behavior investigated in order clarify the mechanism of from viewpoint roles cementite and retained γ. (1) Coarse fine globular that nucleate along boundaries are formed above AC3 temperature. size distribution reheating. (2) by growth, impingement,...
Poly(vinyl alcohol) cast gels (PVA gels) were prepared at different gelation temperatures, Tgel, and the swelling ratio mechanical properties characterized by equilibrium weight measurement, a uniaxial loading test, tearing force cyclic compression test room temperature. The results showed that decreased both breaking stress energy increased with an increase in Tgel. deformation of 50% reduction its original size monotonically, while hysteresis loss was not affected To understand these...
Abstract Human histone H2A.B (formerly H2A.Bbd), a non-allelic H2A variant, exchanges rapidly as compared to canonical and preferentially associates with actively transcribed genes. We found that transiently accumulated at DNA replication repair foci in living cells. To explore the biochemical function of H2A.B, we performed nucleosome reconstitution analyses using various lengths DNA. Two types nucleosomes, octasome hexasome, were formed 116, 124, or 130 base pairs (bp) only was 136 146 bp...
J-PARC Center, Japan Atomic Energy Agency (JAEA), Tokai, Ibaraki 319-1195, Research Center for Neutron Science and Technology, Comprehensive Organization Society (CROSS), 319-1106, High Accelerator (KEK), Tsukuba, 305-0801, Reactor Institute, Kyoto University (KURRI), Kumatori, Osaka 590-0494,
Neuroinflammation essentially involves an activation of glial cells as the cause/effect neurodegenerative diseases such Alzheimer's disease (AD). Plasmalogens (Pls) are glycerophospholipids constituting cellular membranes and play significant roles in membrane fluidity processes like vesicular fusion signal transduction. Intraperitoneal (i.p.) injection lipopolysaccharide (LPS, 250 μg/kg) for 7 days resulted morphological changes increase number Iba‐1 + microglia showing neuroinflammation...
Small-angle neutron scattering (SANS) was used to examine dilute solutions of a poly(quinoxaline-2,3-diyl) (PQX) with (R)-2-octyloxymethyl side chains in deuterated THF or mixture 1,1,2-TCE and (8/2, v/v), which the PQX adopts pure P- M-helical structures. The structures that were obtained based on SANS experiments combination theoretical calculations suggest THF, chiral P-helical are extended, whereas 1,1,2-TCE/THF mixture, folded. Consequently, should be preferred good solvents such as...
Abstract The nucleosome is a fundamental unit of chromatin in which about 150 base pairs DNA are wrapped around histone octamer. overlapping di-nucleosome has been proposed as product remodeling the transcription start site, and previously found unit, 250 continuously bind to core composed hexamer an In present study, our genome-wide analysis human cells suggests another higher stacking structure, tri-nucleosome, wraps 300-350 base-pairs region downstream certain sites actively transcribed...
We characterize shear-induced states of order and disorder in a symmetric lamellae forming poly(cyclohexylethylene) (C)−polyethylene (E) CECEC pentablock copolymer. When subjected to large amplitude reciprocating shear strain (γ = ±600%) the ordered state, sample rapidly aligns perpendicular orientation with long-range order. The order−disorder transition temperature TODT(γ̇) decreases increasing rate γ̇, such that γ̇ 7 ± 1 s-1 is sufficient material nearly 30 °C below quiescent TODT....
Three kinds of lactic acid bacteria were isolated from spoiling cooked meat products stored below 10 degrees C. They identified as Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis lactis, and citreum. All three strains grew well in MRS broth at In particular, L. citreum even 4 C, their doubling times 23.6 51.5 h, respectively. On the other hand, although initially detection limit (<10 CFU/g) model products, bacterial counts increased to 10(8) CFU/g C after 7 12 days.