AMYLOIDOSIS, a rare disease of unknown etiology, is characterized by deposition amorphous hyaline-like material in connective tissues and slow involvement various organs. The clinical manifestations depend upon the rapidity involvements location lesions. primary type amyloidosis affects mainly mesodermal origin, especially heart, tongue, lungs, striated muscle, gastrointestinal tract, blood vessels; death usually due to cardiac changes. Nervous system sporadic cases has been reported several...

Plasmalogens (Pls) reportedly decreased in postmortem brain and the blood of patients with Alzheimer's disease (AD). Recently we showed that intraperitoneal administration Pls improved cognitive function experimental animals. In present trial, tested efficacy oral scallop-derived purified respect to changes mild AD impairment (MCI).The study was a multicenter, randomized, double-blind, placebo-controlled trial 24weeks. Participants were 328 aged 60 85years who had 20 27 points Mini Mental...

Abstract Background Neuroinflammation involves the activation of glial cells in neurodegenerative diseases such as Alzheimer’s disease (AD). Plasmalogens (Pls) are glycerophospholipids constituting cellular membranes and play significant roles membrane fluidity processes vesicular fusion signal transduction. Methods In this study preventive effects Pls on systemic lipopolysaccharide (LPS)-induced neuroinflammation were investigated using immunohistochemistry, real-time PCR methods analysis...

Adenyl cyclase activity in normal human muscle is stimulated four to seven times by epinephrine and about 18 sodium fluoride. The enzyme present all particulate fractions of (myofibrils, mitochondria, sarcoplasmic reticulum) but not the soluble phase. highest specific found isolated plasma membrane. Basal was from patients with Duchenne or facioscapulohumeral dystrophy response 30% fluoride half normal. In polymyositis, responses were normal, intermediate values myotonic dystrophy....

Plasmalogens (Pls), a kind of glycerophospholipids, have shown potent biological effects but their role in hippocampus-dependent memory remained mostly elusive. Here, we first report Pls can enhance endogenous expression brain-derived neurotrophic factor (Bdnf) the hippocampus and promotes neurogenesis associated with improvement learning mice. Genomic proteomic studies revealed that enhanced recruitment CREB transcription onto murine Bdnf promoter region via upregulating ERK-Akt signaling...

Neuroinflammation essentially involves an activation of glial cells as the cause/effect neurodegenerative diseases such Alzheimer's disease (AD). Plasmalogens (Pls) are glycerophospholipids constituting cellular membranes and play significant roles in membrane fluidity processes like vesicular fusion signal transduction. Intraperitoneal (i.p.) injection lipopolysaccharide (LPS, 250 μg/kg) for 7 days resulted morphological changes increase number Iba‐1 + microglia showing neuroinflammation...

Introduction. Parkinson's disease (PD) is the second most common neurodegenerative after Alzheimer's (AD). With ageing of population, frequency PD expected to increase dramatically in coming decades. L-DOPA (1,3,4-dihydroxyalanine) effective drug symptomatic treatment PD. Nonmotor symptoms include sleep problems, depression, and dementia, which are not adequately controlled with dopaminergic therapy. Here, we report efficacy oral administration scallop-derived ether phospholipids some...

• Adenosine triphosphatase (ATPase) activity in erythrocyte membranes from patients with Duchenne dystrophy was inhibited by ouabain less than normal individuals assay systems containing high or low contents of salt. Epinephrine and cyclic adenosine monophosphate increased total ATPase all samples, epinephrine restored sensitivity to the membranes. Basal adenyl cyclase about twice that controls. stimulated two three times, but did not stimulate enzyme These differences may reflect a genetic...

Cultured muscle cells from patients with Duchenne muscular dystrophy differed of normal individuals and other diseases. In cells, basal activity adenyl cyclase myotubes was higher not stimulated significantly by epinephrine or isoproterenol, as it in fused control the response to fluoride less. The genetic defect this disease may be an abnormality surface membrane muscle.

Dietary sphingolipids (SL) inhibit colon carcinogenesis, reduce serum cholesterol, and improve skin barrier function are considered to be "functional lipids". For comparative determination of the effects SL with different chemical compositions on lipid metabolism its related hepatic gene expression, Zucker fatty rats were fed pure sphingomyelin (SM) animal origin glucosylceramide (GC) plant origin. After 45 days, SM GC diets led significant reductions in plasma non-HDL cholesterol. Both...

Plasmalogens are a subclass of glycerophospholipid containing vinyl-ether bond at the sn-1 position glycerol backbone. Ethanolamine-containing plasmalogens (plasmalogens) major constituents cellular membranes in mammalian cells and de novo synthesis largely contributes to homeostasis plasmalogens. Plasmalogen biosynthesis is regulated by feedback mechanism that senses plasmalogen level inner leaflet plasma membrane regulates stability fatty acyl-CoA reductase 1 (Far1), rate-limiting enzyme...

There are several reports indicating a decrease of ethanolamine plasmalogen (pl-PE) in brain tissues and serum patients with Alzheimer's disease (AD). The present study aimed to examine the composition erythrocyte phospholipids including pl-PE AD.A high-performance liquid chromatography (HPLC) method that can separate intact plasmalogens all other phospholipid classes by single chromatographic run was used.The ratios pl-PE, phosphatidylethanolamine (PE) phosphatidylserine (PS) sphingomyelin...

Many disorders with plasmalogen deficiency have been reported. Replenishment or replacement of tissue plasmalogens these would be beneficial to the patients disorders, but effects dietary on mammals not Plasmalogens were purified from chicken skin. The consisted 96.4% ethanolamine (PlsEtn), 2.4% choline (PlsCho) and 0.5% sphingomyelin (SM). A diet containing 0.1% (PlsEtn diet) was given rats. Relative composition phospholipids measured by a high performance liquid chromatography (HPLC)...

A method for the simultaneous purification of plasmalogens and sphingomyelin (SM) in human erythrocytes is described. Treatment total lipids with n-hexane/acetone (1:1 v/v) resulted selective precipitation SM. Both supernatant precipitate fractions were incubated a phospholipase A(1) (PLA1) from Aspergillus orizae 3.5 h. The PLA1-treated extracted n-hexane/isopropanol, hexane layer was obtained using Na(2)SO(4) solution, further washed water. At this step, relative concentration 92%...

Abstract Plasmalogens are glycerophospholipids that contain a vinyl ether bond at the sn-1 position of glycerol backbone instead an ester bond. indicated to have many important functions in mammalian cells. On other hand, it is suggested some gut microbiota plays probiotic human health. Presence plasmalogens Clostridium strains well-known, but presence Bifidobacterium longum ( B. ) strain, one most microbiota, has not been reported. We identified lipid extract from species, animalis (B....

Effects of oxidative stress on intact human erythrocytes were investigated using tert-butyl hydroperoxide (tBHP). Exposure to tBHP caused a marked decrease in filterability time-dependent manner. Erythrocytes exposed also show an increase mean corpuscular volume and remarkable formation methemoglobin (met-Hb) without any appearance hemichromes that form Heinz bodies. High performance liquid chromatography demonstrated the tBHP-treated exhibited apparent membrane phospholipid,...

Ethanolamine ether phospholipid (eEtnGpl) and choline (eChoGpl) are present in human plasma or serum, but the relative concentration of phospholipids is very low as compared to those other tissues. Nowadays, measurement depends on tandem mass spectrometry (LC/MS/MS), a system for LC/MS/MS generally too expensive usual clinical laboratories. Treatment with phospholipase A1 (PLA1) causes complete hydrolysis diacylphospholipids, remain intact. After treatment PLA1, both eEtnGpl eChoGpl detected...