A5079793591- Ruminant Nutrition and Digestive Physiology
- Reproductive Physiology in Livestock
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Gut microbiota and health
- Animal health and immunology
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Probiotics and Fermented Foods
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Milk Quality and Mastitis in Dairy Cows
- Genetic and phenotypic traits in livestock
- Aquaculture disease management and microbiota
- Reproductive System and Pregnancy
- Legionella and Acanthamoeba research
- Antimicrobial agents and applications
- Effects of Environmental Stressors on Livestock
- Nanocomposite Films for Food Packaging
- Plant and fungal interactions
- Microbial Inactivation Methods
University of Florida
2016-2025
Florida Agricultural and Mechanical University
2024
Florida College
2024
University of Idaho
2022
Kyung Hee University
2018-2020
Washington University in St. Louis
2016-2019
University of Wisconsin–Madison
2005-2006
Madison Area Technical College
2005
Chonnam National University
1997-1998
The objective of this study was to evaluate the progression uterine microbiota from calving until establishment metritis. Uterine swabs (n = 72) collected at 0, 2, and 6 ± 2 days postpartum (dpp) 12 metritic healthy cows were used for metagenomic sequencing 16S rRNA gene on Illumina MiSeq platform. A heat map showed that established calving. changed rapidly 0 dpp, with a decrease in abundance Proteobacteria an increase Bacteroidetes Fusobacteria, which dominant cows. composition shared;...
Multiple synergistic factors affect the development and composition of mammalian gut microbiota, but effects host genetics remain unclear. To illuminate role on we employed animals with a graduated spectrum genetic variation minimal environmental influences. We bred 228 calves linearly varying breed from 100% Angus (Bos taurus) to Brahman indicus), as proxy for variation, then raised offspring in same environment identical diets. hypothesized each would harbor distinct microbiota due...
Abstract The gut microbiota is a complex ecological community that plays multiple critical roles within host. Known intrinsic and extrinsic factors affect structure, but the influence of host genetics understudied. To investigate role upon we performed longitudinal study in which evaluated hindgut its association with animal growth immunity across life. We three different stages an Angus-Brahman multibreed population graduated spectrum genetic variation, raised under variable environmental...
Abstract Antibiotic-resistant microorganisms (ARMs) are widespread in natural environments, animals (wildlife and livestock), humans, which has reduced our capacity to control life threatening infectious disease. Yet, little is known about their transmission pathways, especially at the wildlife-livestock interface. This study investigated potential of ARMs antibiotic resistance genes (ARGs) between cattle wildlife by comparing gut microbiota ARG profiles feral swine ( Sus scrofa ), coyotes...
Antibiotic resistance is growing exponentially, increasing public health concerns for humans and animals. In the current study, we investigated antimicrobial features of chitosan microparticles (CM), engineered from by ion gelation, seeking potential application treating infectious disease caused multidrug resistant microorganisms. CM showed excellent activity against a wide range microorganisms, including clinically important antibiotic-resistant pathogens without raising mutants in serial...
Calf diarrhea is one of the most concerning challenges facing both dairy and beef cattle industry. Maintaining healthy gut microbiota essential for preventing gastrointestinal disorders. Here, we observed significantly less bacterial richness in abnormal feces with watery or hemorrhagic morphology compared to normal solid feces. The showed high relative abundances Osllospiraceae, Christensenellaceae, Barnesiella, Lactobacillus, while contained more taxa Negativicutes, Tyzzerella,...
Objective This study aimed to evaluate bacterial and host factors causing a fever in cows with metritis. For that, we investigated uterine microbiota using metagenomic sequencing of the 16S rRNA gene (Study 1), immune response parameters 2) metritic without fever. Principal Findings (Study1) Bacterial communities were similar between MNoFever MFever groups based on distance metrics relative abundance bacteria. Metritic showed greater prevalence Bacteroidetes, Bacteroides Porphyromonas...
Significance Legionella pneumophila is a bacterial pathogen that causes potentially fatal form of pneumonia (Legionnaires' disease) by replicating within human alveolar macrophages, immune cells typically kill microorganisms in the lung. avoids intracellular death injecting many protein effectors into host cell cytoplasm via specialized export apparatus called “Dot/Icm type IVB system.” We showed Dot/Icm (defect organelle trafficking/intracellular multiplication) secretion system restricted...
Heat stress in the dry period affects immune status of dairy cows subsequent lactation. We hypothesized that cooling during improves response to postpartum intramammary infection (IMI) by environmental pathogens such as Streptococcus uberis. Cows were dried off 46 d before expected calving and assigned (CL, n = 15) or heat (HT, 15). Cooled housed with sprinklers, fans, shade, whereas HT group had only shade. All cooled postpartum. Rectal temperature (RT) respiration rate (RR) recorded thrice...
Our objectives were to determine the effects of an injectable formulation calcitriol on mineral metabolism and immune function in postpartum Holstein cows that received acidogenic diet prepartum minimize hypocalcemia. In experiment 1, within 6 h calving (0, 200, or 300 μg) dose needed increase plasma concentrations Ca; μg was sufficient sustain Ca for at least 3 d. 2, multiparous assigned randomly receive only vehicle (control, n = 25) (n subcutaneously first after calving. Blood sampled...