A5035330385- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- HIV Research and Treatment
- Epigenetics and DNA Methylation
Ludwig-Maximilians-Universität München
2016-2018
Center for Integrated Protein Science Munich
2016-2018
Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist a balancer and reporter part separate the gas phase. An ingenious distribution stable isotopes provides multiple identical molecular weight but different mass groups, allowing relative samples one measurement. Here we report new reagent, where are linked by sulfoxide group, which, based on pyrolysis, leads to easy...
A deeper investigation of the chemistry that occurs on newly discovered epigenetic DNA bases 5-hydroxymethyl-(hmdC), 5-formyl-(fdC), and 5-carboxy-deoxycytidine (cadC) requires chemical tool compounds, which are able to dissect different potential reaction pathways in cells. Here we report 2'-(R)-fluorinated derivatives F-hmdC, F-fdC, F-cadC, resistant removal by base excision repair, good substrates for polymerases TET enzymes. This result shows fluorinated compounds ideal substances...
Abstract Die moderne Proteomik erfordert Reagentien für eine akkurate Quantifizierung von Peptiden in komplexen Proben. Peptide werden üblicherweise mit isobaren Markierungen versehen, die aus einem Massenausgleicher und Reporter bestehen, der Gasphase gespalten werden. Eine geschickte Verteilung stabilen Isotope liefert mehrere identischem Molekulargewicht, jedoch einer abweichenden Masse Reportergruppen, was relative mehrerer Proben einzigen Messung ermöglicht. Hier wird ein neues isobares...