A5008085121- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Photochromic and Fluorescence Chemistry
- Metal complexes synthesis and properties
- X-ray Diffraction in Crystallography
- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Cyclopropane Reaction Mechanisms
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- HIV Research and Treatment
- Crystallization and Solubility Studies
- bioluminescence and chemiluminescence research
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Crystallography and molecular interactions
Max Planck Institute of Biochemistry
2013-2017
Ludwig-Maximilians-Universität München
2013-2016
Center for Integrated Protein Science Munich
2016
LMU Klinikum
2013
Abstract We report the development of a new heterobase that is held together through reversible bonding. The so‐formed cross‐link adds strong stabilization to DNA duplex. Despite this, opens and closes imine Moreover, even enzymatic incorporation possible. principle can be used stabilize duplexes nanostructures otherwise require high salt concentrations, which may hinder biological applications.
A straightforward and efficient synthesis of γ-fluorophore labeled nucleoside 5′-triphosphates is reported, using Cu(<sc>i</sc>) catalyzed Huisgen cycloadditions.
The development of metal base pairs is immense importance for the construction DNA nanostructures. Here we report synthesis a biaryl pyrazole-phenol nucleoside that forms in stable self-pair upon complexation Cu(II) ion. A sequence with five consecutive pyrazole nucleotides allows ions row.
A deeper investigation of the chemistry that occurs on newly discovered epigenetic DNA bases 5-hydroxymethyl-(hmdC), 5-formyl-(fdC), and 5-carboxy-deoxycytidine (cadC) requires chemical tool compounds, which are able to dissect different potential reaction pathways in cells. Here we report 2'-(R)-fluorinated derivatives F-hmdC, F-fdC, F-cadC, resistant removal by base excision repair, good substrates for polymerases TET enzymes. This result shows fluorinated compounds ideal substances...
Abstract Synthetic mRNAs are promising candidates for a new class of transformative drugs that provide genetic information patients’ cells to develop their own cure. One key advancement so‐called druggable was the preparation chemically modified mRNAs, by replacing standard bases with bases, such as uridine pseudouridine, which can ameliorate immunogenic profile and translation efficiency mRNA. Thus introduction nucleobases foundation clinical use mRNAs. Herein we describe modular simple...
We report a chemical DNA–DNA ligation method based on copper-catalyzed azide–alkyne cycloaddition (CuAAC). demonstrate that ion addition dramatically influences the efficiency of so-called click reaction. Even without any further additions, such as typically splint oligonucleotides for preorganization, "click ligation" yields up to ∼83% product byproducts. Additionally, purification desired is straightforward. In comparison enzymatic methods used introduce adapters into, e.g., mRNA library...
Tamed electrons: To manipulate a protein photocycle in directed manner, the flavoprotein dodecin was endoscopically modified at its key amino acid tryptophan with substituents carefully selected by their structural and electronic influence. The approach is ideal precision of rational engineering, allows correlating ionization potentials electron transfer rates Marcus model. As service to our authors readers, this journal provides supporting information supplied authors. Such materials are...
Abstract Wir berichten von der Entwicklung eines “Heterobasenpaars”, das durch Bildung einer Schiff'schen Base kovalent verknüpft wird. Diese Vernetzung erzeugt eine hohe Stabilisierung DNA‐Doppelsträngen, aber kann aufgrund reversibler Iminchemie geöffnet werden. Darüber hinaus ist sogar enzymatische Einbau möglich. Unser Heterobasenpaar eröffnet neue Möglichkeiten zur DNA‐Doppelsträngen und ‐Nanostrukturen unter Bedingungen, die kompatibel mit biologischen Sytemen sind.
Here we describe the development of a novel click chemistry-based method for generation and amplification full-length cDNA libraries from total RNA, while avoiding need problematic template-switching (TS) reactions. Compared with prior efforts, our involves neither random priming nor stochastic termination, thus enabling transcripts that were previously inaccessible via related RNA sequencing techniques. A key modification involving use PCR primers containing two overhanging 3'-nucleotides...
Gezähmte Elektronen: Es galt, einen Photozyklus gezielt zu verändern. Dazu wurde das Flavoprotein Dodecin in der Schlüsselaminosäure Tryptophan endoskopischer Weise mit Substituenten – gewählt nach strukturellen und elektronischen Einflüssen verändert. Dieser Ansatz ist ideal Präzision des rationalen Protein-Engineerings erlaubt die Korrelation Ionisierungspotentials von Elektrontransferraten einem Marcus-Modell.
Biorthogonal labelling with fluorescent small molecules is an indispensable tool for diagnostic and biomedical applications. In dye-based 5-ethynyl-2'-deoxyuridine (EdU) cell proliferation assays, augmentation of the signal entails overall enhancement in sensitivity quality method. To this end, a rapid, divergent synthetic procedure that provides ready-to-click pH-insensitive rhodamine dyes exhibiting outstanding brightness was established. Compared to shortest available synthesis related...
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