A5025916963- Dental materials and restorations
- Dental Erosion and Treatment
- Endodontics and Root Canal Treatments
- Oral microbiology and periodontitis research
- Dental Implant Techniques and Outcomes
- Laser Applications in Dentistry and Medicine
- Dental Trauma and Treatments
- Orthodontics and Dentofacial Orthopedics
- Dental Health and Care Utilization
- Biochemical Analysis and Sensing Techniques
- Morinda citrifolia extract uses
- Collagen: Extraction and Characterization
- Chromatography in Natural Products
- Synthesis of Organic Compounds
- Finance, Taxation, and Governance
- Bone and Dental Protein Studies
- Natural product bioactivities and synthesis
- Biochemical effects in animals
- Additive Manufacturing and 3D Printing Technologies
- Dental Anxiety and Anesthesia Techniques
- Phytochemicals and Antioxidant Activities
- Aluminum Alloys Composites Properties
- CNS Lymphoma Diagnosis and Treatment
- Environmental Chemistry and Analysis
- Viral-associated cancers and disorders
University of Iowa
2016-2024
Hospital Central de la Cruz Roja San José y Santa Adela
2023
University of Illinois Chicago
2010-2020
Universidade de Passo Fundo
2015-2016
Universidade Estadual de Campinas (UNICAMP)
2011-2016
Universidade de Brasília
2016
Universidade Estadual Paulista (Unesp)
2010-2012
Methodist University of Piracicaba
2012
Fundación Ciencias Exactas y Naturales
1999
Consejo Nacional de Investigaciones Científicas y Técnicas
1999
The co-expression of MMPs and cysteine cathepsins in the human dentin-pulp complex indicates that both classes enzymes can contribute to endogenous proteolytic activity dentin. Chlorhexidine (CHX) is an efficient inhibitor MMP activity. This study investigated whether CHX could also inhibit present inhibitory profile on dentin-extracted recombinant (B, K, L) was monitored fluorogenic substrates. rate substrate hydrolysis spectrofluorimetrically measured, constants were calculated. Molecular...
Degradation of dentin matrix components within caries has been correlated with the activity host-derived proteases, such as metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship not fully established, we hypothesized that abundance MMPs CTs in caries-affected must be higher than intact dentin. To test premise, obtained 5 slices (200 µm) from teeth (1 slice/tooth) individually incubated them primary antibodies for CT-B, CT-K, MMP-2, or MMP-9. Negative controls were...
Abstract Objectives: To characterize the interaction of 1‐Ethyl‐3‐[3‐dimethylaminopropyl] carbodiimide Hydrochloride (EDC) with dentin matrix and its effect on resin‐dentin bond. Methods: Changes to stiffness demineralized fragments treated EDC/ N ‐hydroxysuccinimide (NHS) in different solutions were evaluated at time points. The resistance against enzymatic degradation was indirectly by ultimate tensile strength (UTS) test or not EDC/NHS subjected collagenase digestion. Short‐ long‐term...
Although proanthocyanidins (PACs) modify dentin, the effectiveness of different PAC sources and correlation with their specific chemical composition are still unknown. This study describes profiling natural PAC-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography (UHPLC) to determine overall these and, in parallel, comprehensively evaluate effect on dentin properties. The total polyphenol content was determined (as gallic acid equivalents) Folin-Ciocalteau...
Oligomeric proanthocyanidins (OPACs) are potent and renewable natural bioactives possible to be refined into chemically standardized mixtures for biological applications. Herein, we found that multiscale interactions of OPACs with the dentin matrix create tight biointerfaces hydrophobic methacrylate adhesives on wet surfaces. An enriched mixture OPACs, a known phytochemical profile, was produced from grape seed crude extract ( Vitis vinifera; [e-GSE]) applied matrices determine changes...
Hydrogen peroxide is an oxidative agent commonly used for dental bleaching procedures. The structural and biochemical responses of enamel, dentin, pulp tissues to the in vivo human (n = 20) premolars were investigated this study. Atomic force microscopy (AFM) was observe enamel nanostructure. chemical composition dentin analyzed by infrared spectroscopy (FTIR). enzymatic activities cathepsin B matrix metalloproteinases (MMPs) monitored with fluorogenic substrates. amount collagen measured...
Proanthocyanidins (PACs) are secondary plant metabolites that mediate nonenzymatic collagen cross-linking and enhance the properties of based tissue, such as dentin. The extent nature is influenced by composition specific chemical structure bioactive compounds present in certain PAC-rich extracts. This study investigated effect molecular weight stereochemistry polyphenol on two important dentin, biomechanics, biostability. For that, purified phenols, a phenolic acid, some its derivatives...
The ability of certain oligomeric proanthocyanidins (OPACs) to enhance the biomechanical properties dentin involves collagen cross-linking 1.3–4.5 nm wide space via protein–polyphenol interactions. A systematic interdisciplinary search for bioactive principles pine bark has yielded trimeric PAC, ent-epicatechin-(4β→8)-epicatechin-(2β→O→7,4β→8)-catechin (3), representing hitherto most potent single chemical entity capable enhancing stiffness. Building case from two congeneric PAC dimers, a...
It has been hypothesized that cysteine cathepsins (CTs) along with matrix metalloproteases (MMPs) may work in conjunction the proteolysis of mature dentin matrix. The aim this study was to verify simultaneously distribution and presence B (CT-B) K (CT-K) partially demineralized dentin; further evaluate activity CTs MMPs same tissue. CT-B CT-K sound human assessed by immunohistochemistry. A double-immunolabeling technique used identify, at once, occurrence those enzymes dentin. Activities...
The structurally complex oligomeric proanthocyanidins (OPACs) are promising biomimetic agents, capable of strengthening the macromolecular backbone teeth via intermolecular and intermicrofibrillar cross-linking. This study establishes analytical methods determining absolute configuration catechin-type monomeric units underivatized OPACs. preserves capacity their biological evaluation, aimed at understanding inevitably stereospecific interactions between OPACs dentin collagen. Guided by...
The objective of this study was to evaluate the effects glycolic acid (GA) (with pH 1.2 and 5) ethylenediaminetetraacetic (EDTA) on chemical mechanical properties dentin investigate potential use GA as final irrigant in root canal therapy. Specifically, changes microhardness, smear layer removal, erosion, mineral content distribution, apatite/collagen ratio flexural strength mineralized treated with were assessed. Saline solution used a negative control. Knoop microhardness (KHN) measured...