A5010570514- Mesenchymal stem cell research
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- RNA and protein synthesis mechanisms
- Angiogenesis and VEGF in Cancer
- Cancer-related Molecular Pathways
- Chromatin Remodeling and Cancer
- Forensic and Genetic Research
- Telomeres, Telomerase, and Senescence
- Cerebrovascular and Carotid Artery Diseases
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Mechanisms of cancer metastasis
- Protein Degradation and Inhibitors
- Forensic Anthropology and Bioarchaeology Studies
- Neuroblastoma Research and Treatments
- Paleopathology and ancient diseases
- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Cardiac Valve Diseases and Treatments
- Autophagy in Disease and Therapy
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Genetic Neurodegenerative Diseases
University of Campania "Luigi Vanvitelli"
2011-2020
University of Naples Federico II
2008-2017
Lund University
2006
Magna Graecia University
2006
Leeds General Infirmary
2006
University of Manchester
2006
University of Leeds
2006
Regione Campania
2001
Istituto di Farmacologia Traslazionale
1999
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
1999
Occlusive vascular disease is a widespread abnormality leading to lethal or debilitating outcomes such as myocardial infarction and stroke. It part of atherosclerosis evoked by clinical procedures including angioplasty grafting saphenous vein in bypass surgery. A causative factor the switch smooth muscle cells an invasive proliferative mode, neointimal hyperplasia. Here we reveal importance this process TRPC1, homolog Drosophila transient receptor potential. Using 2 different vivo models...
Cellular senescence is the permanent arrest of cell cycle, physiologically related to aging and aging-associated diseases. Senescence also recognized as a mechanism for limiting regenerative potential stem cells protect from cancer development. The program realized through autocrine/paracrine pathways based on activation peculiar senescence-associated secretory phenotype (SASP). We show here that conditioned media (CM) senescent mesenchymal (MSCs) contain set secreted factors are able induce...
// Nicola Alessio 2 , Stefania Del Gaudio Capasso Giovanni Di Bernardo Salvatore Cappabianca 4 Marilena Cipollaro Gianfranco Peluso 3 Umberto Galderisi 1, 2, 1 Sbarro Institute for Cancer Research and Molecular Medicine, Center Biotechnology, Temple University, Philadelphia, PA 19107-6799, USA Department of Experimental Biotechnology Biology Section, Second University Naples, Naples 80138, Italy Bioscience Bioresources, CNR, “F. Magrassi – A. Lanzara” Correspondence to:...
// Stefania Capasso 2 , Nicola Alessio Tiziana Squillaro Giovanni Di Bernardo Mariarosa A. Melone 3,4 Marilena Cipollaro Gianfranco Peluso 3 and Umberto Galderisi 1,2,3 1 Sbarro Institute for Cancer Research Molecular Medicine, Center Biotechnology, Temple University, Philadelphia, PA, USA Department of Experimental Biotechnology Biology Section, Second University Naples, Italy Bioscience Bioresources, CNR, 4 Clinical Medicine Surgery, Division Neurology, Correspondence to: Peluso, email:...
Abstract In recent years several reports have claimed to demonstrate trans‐differentiation, namely that stem cells been derived from a given tissue and differentiated into phenotypes characteristic of different tissues following transplantation or in vitro treatment. For example, the mesenchymal cells, also referred as marrow stromal (MSCs), present bone marrow, induced differentiate neurons. We decided investigate this phenomenon more depth by molecular morphological follow‐up. analyzed...
Endometriosis is a chronic disease characterized by the presence of ectopic endometrial tissue outside uterus with mixed traits benign and malignant pathology. In this study we analyzed in endometriotic tissues differential expression panel genes that are involved preservation stemness status consequently considered as markers stem cell presence. The profiles 13 (SOX2, SOX15, ERAS, SALL4, OCT4, NANOG, UTF1, DPPA2, BMI1, GDF3, ZFP42, KLF4, TCL1) were reverse transcription-polymerase chain...
Mesenchymal stem cells (MSCs) are of particular interest because they being tested using cell and gene therapies for a number human diseases. MSCs represent rare population in tissues. Therefore, it is essential to grow vitro before putting them into therapeutic use. This compromised by senescence, limiting the proliferative capacity MSCs. We analyzed senescence rat MSCs, this animal widespread model preclinical therapy studies. After initial expansion, showed an increased growth doubling...
Human endometrium is a highly dynamic tissue, undergoing periodic growth and regression at each menstrual cycle. Endometriosis frequent chronic pathological status characterized by endometrial tissue with an ectopic localization, causing pelvic pain infertility variable clinical presentation. In addition, there well-established evidence that, although endometriosis considered benign, it associated increased risk of malignant transformation in approximately 1.0% affected women, the...
Overweight status should not be considered merely an aesthetic concern; rather, it can incur health risks since may trigger a cascade of events that produce further fat tissue through altered levels circulating signaling molecules.We decided to investigate the influence overweight individuals' sera on in vitro MSC proliferation and differentiation.We observed incubation bone marrow stromal cells with individuals promotes adipogenic differentiation MSCs while partially impairing proper...
Histone deacetylase inhibitors (HDACi) have received a great amount of attention for their antitumoral properties. Suberoyl anilide hydroxamic acid (SAHA) and MS-275 are among the more promising HDACi cancer treatments. Although these compounds exert low toxicity on normal cells, therapies based molecules can cause side effects that greatly impair functions bone marrow microenvironment. This is complex system contains several types stem such as mesenchymal cells (MSCs). We conducted...
Previous studies on BAV (bicuspid aortic valve)-related aortopathy, whose aetiology is still debated, have focused mainly severe dilatations. In the present study, we aimed to detect earlier signs of aortopathy. Specimens were collected from ‘concavity’ (lesser curvature) and ‘convexity’ (greater mildly dilated AAs (ascending aortas; diameter ≤4 cm) with stenotic TAV (tricuspid valve) or donor normal aortas. submitted morphometry, immunohistochemistry differential gene-expression analysis,...
Rationale: The pathogenesis of bicuspid aortic valve (BAV)–associated aortopathy is poorly understood, and no prognostic biomarker currently available. Objective: We aimed to identify putative circulating biomarkers pathogenetically prognostically linked aortopathy. Methods Results: By reverse transcription polymerase chain reaction, we evaluated gene expression variations (versus normal aorta) transforming growth factor-β1 (TGF-β1), connective tissue factor, matrix metalloproteinase-2...
To clarify the role and function of N-myc product in cell differentiation apoptosis, we used antisense oligonucleotide technique to inhibit gene expression neuroblastoma cells with different phenotypes: intermediate (I) neuronal (N), or Schwann-glia (S), respectively. The results suggest that operates along pathways. Inhibiting either suppression proliferation induction and/or apoptosis. J. Cell. Biochem. 73:97–105, 1999. © 1999 Wiley-Liss, Inc.
Self-renewal, proliferation and differentiation properties of stem cells are controlled by key transcription factors. However, their activity is modulated chromatin remodeling factors that operate at the highest hierarchical level. Studies on these can be especially important to dissect molecular pathways governing biology cells. SWI/SNF complexes adenosine triphosphate (ATP)-dependent enzymes have been shown required for cell cycle control, apoptosis in several biological systems. The aim...
MECP2 protein binds preferentially to methylated CpGs and regulates gene expression by causing changes in chromatin structure. The mechanism which impaired activity can induce pathological abnormalities the nervous system of patients with Rett syndrome (RTT) is not clearly understood. To gain further insight into role human neurogenesis, we compared neural differentiation process mesenchymal stem cells (MSCs) obtained from a RTT patient healthy donors. We analyzed neuroblastoma cell line...