A5006742911- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Signaling Pathways in Disease
- Protein Kinase Regulation and GTPase Signaling
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Protein Tyrosine Phosphatases
- Neuroscience and Neural Engineering
- Hippo pathway signaling and YAP/TAZ
- PI3K/AKT/mTOR signaling in cancer
- Ion channel regulation and function
- Nuclear Receptors and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Toxin Mechanisms and Immunotoxins
- Neonatal and fetal brain pathology
- Anesthesia and Neurotoxicity Research
- Bone Tissue Engineering Materials
- Neuroblastoma Research and Treatments
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
Ruhr University Bochum
2014-2024
Institute of Cytochemistry and Molecular Pharmacology
2010
Leipzig University
2004
Mario Negri Institute for Pharmacological Research
2001
Max Planck Institute for Brain Research
1993
Ludwig-Maximilians-Universität München
1993
München Klinik
1993
Max Planck Institute of Biochemistry
1977-1992
Max Planck Institute of Psychiatry
1981-1991
Max Planck Institute of Neurobiology
1983-1990
Erythropoietin (EPO) promotes neuronal survival after hypoxia and other metabolic insults by largely unknown mechanisms. Apoptosis necrosis have been proposed as mechanisms of cellular demise, either could be the target actions EPO. This study evaluates whether antiapoptotic can account for neuroprotective Systemic administration EPO (5,000 units/kg body weight, i.p.) middle-cerebral artery occlusion in rats dramatically reduces volume infarction 24 h later, concert with an almost complete...
The intact sciatic nerve contains levels of growth factor (NGF) that are comparable to those densely innervated peripheral target tissues NGF-responsive (sympathetic and sensory) neurons. There, the high NGF reflected by correspondingly mRNANGF levels. In nerve, were very low, thus indicating contribution locally synthesized nonneuronal cells is small. However, after transection an increase up 15-fold in was measured 4-mm segments collected both proximally distally site. Distally site,...
In newborn rats the levels of nerve growth factor (NGF) mRNA (mRNANGF) and NGF receptor (mRNA(rec)) in sciatic were 10 120 times higher, respectively, than adult animals. mRNA(rec) decreased steadily from birth, approaching by third postnatal week, whereas mRNANGF only after first although also reaching week. Transection resulted a marked biphasic increase with time. On proximal side cut, this was confined to area immediately adjacent cut; peripherally, similar present all segments. markedly...
THE effects of BDNF, and NT-4/5 on AMPA receptor-mediated synaptic transmission were investigated with the patch clamp technique applied to embryonic, postnatal rat hippocampal neurones, cultured in serum-free medium. Spatially restricted application neurotrophin-containing solution recorded cells was performed, evoked as well miniature excitatory postsynaptic currents (mepscs) monitored. In approximately 25% neurones tested a transient augmentation currents, increase frequency mepscs...
Abstract In order to obtain information on the physiological regulation of NGF‐synthesis in central nervous system (CNS) we investigated effects a series growth factors (known be present CNS) cultures purified rat astrocytes and compared these with those observed after intraventricular injection same molecules. After preliminary experiments had shown that 10% fetal calf serum (FCS) produced marked increase NGF‐mRNA levels (but neither microglia nor oligodendrocytes) as demonstrated by...
Calcium phosphate has excellent biocompatibility due to its chemical similarity human hard tissue (bone and teeth). In nanoparticulate dispersed form, it can be used as a carrier in biological systems, e.g. transfer nucleic acids or drugs. If such nanoparticles are suitably functionalized with fluorescing dyes, they also for imaging photodynamic therapy.
Lesion of the sciatic nerve caused a rapid increase in c-fos and c-jun mRNA that was followed about 2 hr later by an growth factor (NGF) mRNA. To evaluate whether initial is causally related to subsequent NGF mRNA, we performed experiments with fibroblasts transgenic mice carrying exogenous gene under control metallothionein promoter. In primary cultures these fibroblasts, CdCl2 evoked immediately endogenous slight delay C3H mice, had no effect on levels protooncogenes or NGF. Additional...
The lymphokine interleukin 1 (IL-1) rapidly increases the levels of mRNA encoding nerve growth factor (NGF) in primary cultures fibroblasts isolated from adult rat sciatic nerves. effect IL-1 on NGF-mRNA was not inhibited by cycloheximide, which itself increased NGF-mRNA. From blocking ability actinomycin D, it deduced that action depends continuous RNA synthesis and half-life is about 90 min. Nuclear run-on studies showed that, although enhances low basic transcription, main results...
We have found a regional distribution of IL 1 beta mRNA and activity in the normal adult rat brain, which reveals at least partially colocalization with nerve growth factor (NGF). The predominantly neuronal signal patterns were over granule cells dentate gyrus, pyramidal hippocampus, cerebellum, periglomerular olfactory bulb, dispersed ventromedial hypothalamus frontal cortex. In these areas also highest levels observed. striatum septum much lower (shown for striatum), most likely...
Interleukin-6 (IL-6) is a multifunctional cytokine mediating inflammatory or immune reactions. Here we investigated the possible role of IL-6 in intact lesioned peripheral nervous system using adult gene knockout (IL-6(-/-)) mice. Various sensory functions were tested by applying electrophysiological, morphological, biochemical, and behavioral methods. There was 60% reduction compound action potential branch IL-6(-/-) mice as compared with motor sciatic nerve. Cross sections L5 DRG showed...