A5014527668- Gut microbiota and health
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Infant Nutrition and Health
- Gastrointestinal motility and disorders
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Heat shock proteins research
- Cystic Fibrosis Research Advances
- Yersinia bacterium, plague, ectoparasites research
- Ginseng Biological Effects and Applications
- Advanced Fluorescence Microscopy Techniques
- Escherichia coli research studies
- Inflammatory Bowel Disease
- Diet, Metabolism, and Disease
- Digestive system and related health
- Lipid metabolism and biosynthesis
- Bacteriophages and microbial interactions
Uppsala University
2011-2025
Science for Life Laboratory
2025
University of Freiburg
2001
Abstract Aim The aim of this study was to investigate whether two Lactobacillus reuteri strains (rat‐derived R2 LC and human‐derived ATCC PTA 4659 (4659)) could protect mice against colitis, as well delineate the mechanisms behind protection. Methods Mice were given L. or by gavage once daily for 14 days, colitis induced addition 3% DSS (dextran sulphate sodium) drinking water last 7 days period. severity disease assessed through clinical observations, histological evaluation ELISA...
Superparamagnetic iron oxide nanoparticles (SPIONs) exhibit unique properties for diverse biomedical applications, including drug delivery and diagnostic imaging. Actively targeted SPIONs enhance to diseased sites, reducing side effects enhancing treatment efficacy. However, the development of reproducible functionalization protocols is challenged by erratic behavior in suspensions, such as agglomeration sedimentation. In this study, a method developed systematically optimized attach...
Heat shock proteins (HSPs) are a set of highly conserved that can serve as intestinal gate keepers in gut homeostasis. Here, effects probiotic, Lactobacillus rhamnosus GG (LGG), and two novel porcine isolates, johnsonii strain P47-HY reuteri P43-HUV, on cytoprotective HSP expression barrier function, were investigated IPEC-J2 epithelial cell line model. The cells polarized permeable filter exhibited villus-like phenotype with development apical microvilli. Western blot analysis detected...
Bacterial extracellular membrane vesicles (MV) are potent mediators of microbe-host signals, and they not only important in host-pathogen interactions but also for the between mutualistic bacteria their hosts. Studies MV derived from probiotics could enhance understanding these universal signal entities, here we have studied Limosilactobacillus reuteri DSM 17938 BG-R46. The production increased with cultivation time after oxygen stress. Mass spectrometry-based proteomics analyses revealed...
Abstract Background Intestinal Peyer’s patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes IgA induction in PPs, as well downstream consequences on intestinal microbiota susceptibility to inflammation. Results The cells PPs were separated by size circumvent activation-dependent identification biases...
Pathogenic gut bacteria are common causes of intestinal disease. Enteroids—cultured three-dimensional replicas the mammalian gut—offer an emerging model system to study disease mechanisms under conditions that recapitulate key features tract.
Background Patients suffering from diabetes show defective bacterial clearance. This study investigates the effects of elevated plasma glucose levels during on leukocyte recruitment and function in established models inflammation. Methodology/Principal Findings Diabetes was induced C57Bl/6 mice by intravenous alloxan (causing severe hyperglycemia), or high fat diet (moderate hyperglycemia). Leukocyte studied anaesthetized using intravital microscopy exposed cremaster muscles, where numbers...
Aim To investigate colonic mucus thickness in vivo health and during experimental inflammatory bowel disease. Methods Colitis was induced with 5% DSS drinking water for 8 days prior to experiment, when the descending mucosa of anesthetized rats studied using intravital microscopy. Mucus measured micropipettes attached a micromanipulator. assess contributions NOS prostaglandins regulation thickness, non-selective NOS-inhibitor L-NNA (10 mg/kg bolus followed by 3 mg/kg/h), selective...
Lactobacillus reuteri is a symbiont that inhabits the gastrointestinal (GI) tract of mammals, and several strains are used as probiotics. After introduction probiotic in complex ecosystem like GI tract, keeping track them challenge. The main objectives this study were to introduce reporter proteins would enable vivo vitro detection L. increase knowledge about its interactions with host. We describe for first time cloning codon-optimized genes encoding click beetle red luciferase (CBRluc)...
Obesity is linked to non-alcoholic fatty liver disease and risk factors associated metabolic syndrome. Bilberry (Vaccinium myrtillus) that contains easily fermentable fiber may strengthen the intestinal barrier function, attenuate inflammation modulate gut microbiota composition, thereby prevent obesity development. In current study, lipid metabolism, fat depot, cecal serum short-chain acids (SCFAs) microbiome were evaluated in rats fed bilberries a high-fat (HFD + BB) or low-fat (LFD...
Rationale: Ischemic injuries remain a leading cause of mortality and morbidity worldwide, restoration functional blood perfusion is vital to limit tissue damage support healing. Objective: To reveal novel role macrophages in reestablishment following ischemic injury that can be targeted improve restoration. Methods Results: Using intravital microscopy hindlimb muscle mice, confocal human tissues from amputated legs, we found accumulated perivascularly muscles, where they expressed high...
Intestinal homeostasis is maintained by a hierarchy of immune defenses acting in concert to minimize contact between luminal microorganisms and the intestinal epithelial cell surface. The mucus layer, covering gastrointestinal tract cells, contributes mucosal limiting bacterial invasion. In this study, we used γδ T-cell-deficient (TCRδ(-/-)) mice examine whether how T-cells modulate properties layer. Increased susceptibility TCRδ(-/-) dextran sodium sulfate (DSS)-induced colitis associated...
Scope Emerging evidence suggests that high‐fat diet (HFD) is associated with gut microbiome dysbiosis and related disorders. Bilberry a prebiotic food component known health benefits. Herein, the dynamics of colonic mucus layer during HFD bilberry supplementation are addressed. Methods results The effects on thickness in vivo microbiota composition (Illumina sequencing, quantitative real‐time PCR) investigated young rats fed low‐fat or without bilberries for 8 weeks ( n = 8). induced...
Intravital imaging is an invaluable tool for studying the expanding range of immune cell functions. Only in vivo can complex and dynamic behavior leukocytes their interactions with natural microenvironment be observed quantified. While capabilities high-speed, high-resolution confocal multiphoton microscopes are well documented steadily improving, other crucial hardware required intravital often developed in-house less commonly published detail. In this report, we describe a low-cost,...
Urinary tract infections caused by uropathogenic Escherichia coli are one of the most common infections, and can lead to sepsis or end-stage renal failure. To be able study pathology kidney infection longitudinally, we developed an experimental set up that enables long-term studies immune cell behavior in situ challenged as well unchallenged anesthetized mice. Using highly controlled vacuum stabilize kidney, superficial cortex was continuously visualized with minimal disruption local...
Inflammatory bowel disease (IBD) is a chronic condition resulting in impaired intestinal homeostasis. Current practices for diagnosis of IBD are challenged by invasive, demanding procedures. We hypothesized that proteomics analysis could provide powerful tool identifying clinical biomarkers non-invasive diagnosis. Here, the global proteomes from commonly used vitro and vivo models were analyzed to identify apical luminal proteins can be targeted orally delivered diagnostic agents. Global...
Preliminary results from our laboratory suggest that two strains of the probiotic bacteria Lactobacillus reuteri ameliorate DSS‐induced colitis in mice. The chemokine receptor CX3CR1 is expressed by circulating monocytes and intestinal lamina propria resident cells believed to be macrophages and/or dendritic cells. Mice deficient (CX3CR1GFP/GFP) develop an aggravated colitis, suggesting a role maintenance colonic homeostasis. aim study was investigate if protective mechanism L. R2LC or ATCC...
Abstract Background: Intestinal Peyer’s patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Lactobacillus reuteri R2LC affects B lymphocytes IgA induction in PPs, as well downstream consequences on 28 intestinal microbiota inflammation susceptibility. Results: The cells PPs were separated by size to circumvent activation-dependent identification biases...