A5080534600- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Energy Harvesting in Wireless Networks
- Pluripotent Stem Cells Research
- Renal and related cancers
- Xenotransplantation and immune response
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- AI in cancer detection
- Genomics and Chromatin Dynamics
- Digital Imaging for Blood Diseases
- Genetic factors in colorectal cancer
- Immunodeficiency and Autoimmune Disorders
- Ocular Surface and Contact Lens
- Tumors and Oncological Cases
- Cancer Cells and Metastasis
- Medical Imaging and Analysis
- Biomedical Text Mining and Ontologies
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Respiratory viral infections research
- Corneal Surgery and Treatments
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
Beijing Tongren Hospital
2025
Capital Medical University
2025
Takara (United States)
2025
Genesis Foundation
2023
Case Western Reserve University
2019-2022
Third Affiliated Hospital of Sun Yat-sen University
2020-2022
Sun Yat-sen University
2020-2022
Case Comprehensive Cancer Center
2020
Abstract Recent human decedent model studies 1,2 and compassionate xenograft use 3 have explored the promise of porcine organs for transplantation. To proceed to studies, a clinically ready donor must be engineered its successfully tested in nonhuman primates. Here we describe design, creation long-term life-supporting function kidney grafts from genetically transplanted into cynomolgus monkey model. The was carry 69 genomic edits, eliminating glycan antigens, overexpressing transgenes...
There is a strong rationale to consider future cell therapeutic approaches for cystic fibrosis (CF) in which autologous proximal airway basal stem cells, corrected CFTR mutations, are transplanted into the patient's lungs. We assessed possibility of editing locus these cells using zinc-finger nucleases and have pursued two approaches. The first, mutation-specific correction, footprint-free method replacing mutation with sequences. applied this approach correction ΔF508, demonstrating...
Spermatozoa released from the testis are unable to fertilize an egg without a coordinated process of maturation in lumen epididymis. Relatively little is known about molecular events that integrate this critical progression along male genital ducts man. Here, we use single cell RNA-sequencing construct atlas human proximal We find CFTR, which pivotal normal epididymis fluid transport, most abundant surface epithelial cells efferent and rare clear caput epididymis, suggesting region-specific...
Diagnosing rare diseases remains a critical challenge in clinical practice, often requiring specialist expertise. Despite the promising potential of machine learning, scarcity data on and need for interpretable, reliable artificial intelligence (AI) models complicates development. This study introduces multimodal concept-based interpretable model tailored to distinguish uveal melanoma (0.4-0.6 per million Asians) from hemangioma metastatic carcinoma following practice. We collected...
The CFTR gene lies within an invariant topologically associated domain (TAD) demarcated by CTCF and cohesin, but shows cell-type specific control mechanisms utilizing different cis-regulatory elements (CRE) the TAD. Within respiratory epithelium, more than one cell type expresses molecular controlling its transcription are likely divergent between them. Here, we determine how two extragenic CREs that prominent in epithelial cells lung, regulate expression of gene. We showed earlier these...
Abstract RNAs serve a central role in cellular biology by converting genomic information into effector molecules, either as mRNAs or directly functional RNAs. Measurement of RNA through Sequencing has become an important method to understand biological processes. While it been long appreciated that vast diversity transcripts can be produced alternative splicing, more recent work defined significant contribution alternate and aberrant splicing diseases like cancer, neurodegeneration,...
The cystic fibrosis transmembrane conductance regulator (CFTR) gene lies within a topologically associated domain (TAD) in which multiple cis-regulatory elements (CREs) and transcription factors (TFs) regulate its cell-specific expression. CREs are recruited to the promoter by looping mechanism that depends upon both architectural proteins specific TFs. An siRNA screen identify TFs coordinating CFTR expression airway epithelial cells suggested an activating role for BTB CNC homolog 1...
Organoids are a valuable three-dimensional (3D) model to study the differentiated functions of human intestinal epithelium. They particularly powerful tool measure epithelial transport processes in health and disease. Though biological assays such as organoid swelling intraluminal pH measurements well established, their underlying functional genomics not characterized. Here we combine genome-wide analysis open chromatin by ATAC-Seq with transcriptome mapping RNA-Seq define genomic signature...
Abstract E74‐like factor 5 ( ELF5 ) and ETS‐homologous EHF are epithelial selective ETS family transcription factors (TFs) encoded by genes at chr11p13, a region associated with cystic fibrosis (CF) lung disease severity. controls many key processes in function so its regulatory mechanisms important. Using CRISPR/Cas9 technology, we removed three cis ‐regulatory elements (CREs) from the chr11p13 also activated multiple open chromatin sites CRISPRa airway cells. Deletion of CREs caused subtle...
Robust protocols to examine 3D chromatin structure have greatly advanced knowledge of gene regulatory mechanisms. Here we focus on the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which provides a paradigm for validating models regulation built upon genome-wide analysis. We mechanisms by multiple cis-regulatory elements (CREs) at CFTR coordinate its expression in intestinal epithelial cells. Using CRISPR/Cas9 remove CREs, individually and tandem, followed assays...
Abstract The availability of robust protocols to differentiate induced pluripotent stem cells (iPSCs) into many human cell lineages has transformed research the origins disease. efficacy differentiating iPSCs specific cellular models is influenced by factors including both intrinsic and extrinsic features. Among most challenging generation bronchial epithelium at air‐liquid interface (HBE‐ALI), which gold standard for studies respiratory diseases cystic fibrosis. Here, we perform open...
Cell-specific and developmental mechanisms contribute to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) gene; however, its regulation is poorly understood. Here we use human induced pluripotent stem cells differentiated into pseudostratified airway epithelial study these mechanisms.
Purpose: Increased conjunctival arteries and lymphangions accelerate the cellular immune response in recurrent pterygium, however, which plays a more important role warrants further investigation. The aim of study is to compare roles lymphatic blood vessels pterygium recurrence. Methods: Histological sections from 48 excised pterygia (including 14 Grade 1, 20 2, 3 tissues) were examined. seven nasal epibulbar segments served as normal controls. Blood evaluated compared according microvessel...
Recent human decedent model studies and compassionate xenograft use explored the promise of porcine organs for transplantation. However, lack a clinically compatible donor is preventing xenotransplantation from advancing to clinical studies. Here we describe design, creation, long-term life-supporting function transplanted kidneys genome-edited in nonhuman primates. The was engineered carry total 69 genomic edits that eliminate xenoantigens, overexpress transgenes, inactivate endogenous...