A5101986400- Low-power high-performance VLSI design
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Medical Research and Treatments
- Reproductive System and Pregnancy
- Viral Infections and Immunology Research
- DNA Repair Mechanisms
- Healthcare and Venom Research
- COVID-19 Clinical Research Studies
- Recommender Systems and Techniques
- Parallel Computing and Optimization Techniques
- CRISPR and Genetic Engineering
- Numerical Methods and Algorithms
- Gynecological conditions and treatments
- Information Retrieval and Search Behavior
- Virus-based gene therapy research
- Quantum-Dot Cellular Automata
- Endometriosis Research and Treatment
- SARS-CoV-2 detection and testing
- Genomics and Chromatin Dynamics
- interferon and immune responses
- Chronic Disease Management Strategies
- Influenza Virus Research Studies
- Energetic Materials and Combustion
Boehringer Ingelheim (United States)
2024
Affiliated Hospital of Shaanxi University of Chinese Medicine
2022-2023
Shaanxi University of Chinese Medicine
2013-2023
Nanjing University of Aeronautics and Astronautics
2011-2022
Chinese People's Armed Police Force Engineering University
2022
Chinese People's Armed Police Force
2022
Chongqing Medical University
2021
Chongqing Three Gorges Central Hospital
2020
Chongqing University
2020
Fuzhou General Hospital of Nanjing Military Command
2010-2017
Abstract Background We aim to investigate the profile of acute antibody response in COVID-19 patients, and provide proposals for usage test clinical practice. Methods A multi-center cross-section study (285 patients) a single-center follow-up (63 were performed feature SARS-CoV-2. cohort 52 suspects 64 close contacts enrolled evaluate potentiality test. Results The positive rate IgG reached 100% around 20 days after symptoms onset. median day seroconversion both lgG IgM was 13 Seroconversion...
Two highly conserved double-strand break (DSB) repair pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), function in all eukaryotes. How a cell chooses which pathway to utilize is an area of active research debate. During NHEJ, the DNA-dependent protein kinase (DNA-PK) functions as "gatekeeper" regulating DNA access. Here, we provide evidence that DNA-PK regulates access via its own autophosphorylation. We demonstrated previously autophosphorylation within major...
Marek's disease virus (MDV) causes an acute lymphoproliferative in chickens, resulting T cell lymphomas visceral organs and peripheral nerves. Earlier studies have determined that the repeat regions of oncogenic serotype 1 MDV encode a basic leucine zipper protein, Meq, which structurally resembles Jun/Fos family transcriptional activators. Meq is consistently expressed MDV-induced tumor cells has been suggested as MDV-associated oncogene. To study function we generated rMd5DeltaMeq by...
Recent studies have established that DNA-dependent protein kinase (DNA-PK) undergoes a series of autophosphorylation events facilitate successful completion nonhomologous DNA end joining. Autophosphorylation at sites in two distinct clusters regulates access to end-processing factors and other repair pathways. within the kinase's activation loop activity. Additional (as yet undefined) occur mediate dissociation. Here we provide first evidence major (regulating access) occurs trans. Further,...
The outbreak of coronavirus disease 2019 (COVID-19) caused by SARS CoV-2 is ongoing and a serious threat to global public health. It essential detect the quickly immediately isolate infected individuals. Nevertheless, current widely used PCR immunoassay-based methods suffer from false negative results delays in diagnosis. Herein, high-throughput serum peptidome profiling method based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) developed for...
The protein kinase activity of the DNA-dependent (DNA-PK) is required for repair DNA double-strand breaks (DSBs) via process nonhomologous end joining (NHEJ).However, to date, only target shown be functionally relevant enzymatic role DNA-PK in NHEJ large catalytic subunit DNA-PKcs itself.In vitro, autophosphorylation induces inactivation and dissociation from end-binding component Ku70/Ku80.Phosphorylation within two previously identified clusters phosphorylation sites does not mediate...
Convalescent plasma (CP) transfusion has been indicated as a promising therapy in the treatment for other emerging viral infections. However, quality control of CP and individual variation patients different studies make it rather difficult to evaluate efficacy risk coronavirus disease 2019 (COVID-19). We aimed explore potential therapy, assess possible factors associated with its efficacy. enrolled eight critical or severe COVID-19 from four centers. Each patient was transfused 200-400 mL...
Marek's disease, a lymphoproliferative disease of chickens, is caused by an alphaherpesvirus, virus (MDV). This encodes virokine, vIL-8, with general homology to cellular CXC chemokines such as interleukin-8 (IL-8) and Gro-alpha. To study the function vIL-8 gene, we deleted both copies residing in terminal repeat long internal region viral genome generated mutant deleted, rMd5/DeltavIL-8. Growth kinetics showed that gene dispensable for replication cell culture. In vivo, involved early...
Parkinson's disease (PD) and Parkinsonism are common neurodegenerative disorders with continuously increasing prevalence, causing high global burdens. However, data concerning the comorbidity burden of patients PD or in China lacking. To investigate health condition burden, a total 3367 823 were included from seven tertiary hospitals cities across 2003 to 2012. Their was collected quantified by Elixhauser Comorbidity Index (ECI) Charlson (CCI). The spectra differed between patients. Compared...
Due to its high modularity and carry-free addition, a redundant binary (RB) representation can be used when designing performance multipliers. The conventional RB multiplier requires an additional partial product (RBPP) row, because error-correcting word (ECW) is generated by both the radix-4 Modified Booth encoding (MBE) encoding. This incurs in RBPP accumulation stage for MBE multiplier. In this paper, new modified generator (RBMPPG) proposed; it removes extra ECW hence, saves one stage....
BACKGROUND Inflammation is a major cellular strain causing increased risk of osteo-degenerative diseases. Omega-3 fatty acids have been great source in suppressing inflammation. We investigated the effect α-linolenic acid (ALA) on RANKL-stimulated osteoclast differentiation, LPS-induced and ovariectomized bone loss mice models. MATERIAL AND METHODS The marrow macrophages (BMMs) were isolated from femurs ICR mice, stimulated with RANKL, treated ALA (100, 200, 300 µM). Major analytical methods...
Two major DNA repair pathways, nonhomologous end-joining (NHEJ) and homologous recombination (HR), double-stranded breaks (DSBs) in all eukaryotes. Additionally, several alternative pathways (or subpathways) have been reported that characteristically use short-sequence homologies at the ends to facilitate joining. How a cell chooses which pathway (at any particular DSB) is central largely unanswered question. For one type of DSB, there apparently no choice. DSBs mediated by...
Marek's disease virus (MDV) is an alpha-herpesvirus that causes rapid development of T-cell lymphomas in chickens. MDV-encoded vIL-8 homologous to the cellular IL-8 gene, and its function MDV pathogenesis has yet be determined. Using overlapping cosmid clone–based technology, we have generated deletion mutant virus, rMd5/ΔvIL-8. In vivo experiments with this demonstrated results attenuation induction significantly less gross tumor, both viscera nerves, when compared parental virus....
The neuroprotective agents currently used to treat Alzheimer's disease (AD) often only target one aspect of the process. Therefore, identifying effective drug targets associated with pathogenesis AD is critical for production novel therapeutic strategies. present study aimed investigate underlying mechanisms effects Rg1 on a rat model AD. A double transgenic β‑amyloid (Aβ) precursor protein/PS1 was established, which co‑expressed mutations Aβ plaques and neurofibrillary tangles (NFTs) were...
The t(14;18) chromosomal translocation typically involves breakage at the bcl-2 major breakpoint region (MBR) to cause human follicular lymphoma.A theory explain striking propensity of MBR breaks three CpG clusters within 175-bp invoked activation-induced deaminase (AID).In a test that theory, we used here minichromosomal substrates in pre-B cell lines.Consistent with essential elements found process is indeed highly dependent on DNA methylation sites and AID enzyme create lesions peak...
A parallel decimal multiplier with improved performance is proposed in this paper by exploiting the properties of three different binary coded (BCD) codes, namely redundant BCD excess-3 code (XS-3), overloaded digit set (ODDS) and BCD-4221/5211 code. The signed-digit radix-10 recoding used to recode [-5, 5] from [0, 9]. XS-3 adopted generate multiplicand multiples a carry-free manner. partial products (PPs) are converted ODDS PPs fit product reduction (PPR). In paper, regular PPR tree using...
Ischaemic encephalopathy (IE) is a debilitating condition resulting from stroke that can lead to impaired learning and memory related damage of cholinergic neurons in the hippocampus. The present study used an animal model IE test hypothesis treatment with basic fibroblast growth factor (bFGF) reduce cognitive symptoms by increasing number CA region was created surgically double ligation bilateral common carotid arteries. Three groups Sprague-Dawley rats: sham, bFGF group, were measured for...
Semantically searching and navigating products (e.g., on Taobao.com or Amazon.com) with professional metadata user-generated content from social media is a hot topic in information retrieval recommendation systems, while most existing methods are specifically designed as purely system. In this paper, taking Social Book Search an example, we propose general search-recommendation hybrid system for topic. Firstly, Generalized Content-Based Filtering (GCF) model. model, preference value, which...
Background and purpose Data on the efficacy safety of alteplase for acute ischaemic stroke (AIS) administered 3–4.5 hours after onset symptoms in Chinese patients is limited. We sought to determine whether AIS would benefit from thrombolysis with between 3 4.5 a prospective, multicentre, single-arm trial China. Materials methods Eligible were given 0.9 mg/kg intravenously. The primary endpoint was favourable outcome at months, defined as score 0 or 1 modified Rankin Scale. Thresholds...
A parallel decimal multiplier is proposed in this paper to improve performance by mainly exploiting the properties of three different binary coded (BCD) codes, namely redundant BCD excess-3 code (XS-3), overloaded digit set (ODDS) and BCD-4221/5211 code, hence design referred as hybrid. The signed-digit radix-10 recoding with {-5, 5} (XS-3) representations are used for partial product (PP) generation. In paper, a new reduction (PPR) tree proposed, it consists PPR block, nonfixed size...