A5006124607- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Transcranial Magnetic Stimulation Studies
- Endoplasmic Reticulum Stress and Disease
- Stroke Rehabilitation and Recovery
- Peripheral Neuropathies and Disorders
- Muscle activation and electromyography studies
- Autophagy in Disease and Therapy
- High Altitude and Hypoxia
- Cardiomyopathy and Myosin Studies
- Calpain Protease Function and Regulation
- Acute Ischemic Stroke Management
- Mitochondrial Function and Pathology
- Cerebral Venous Sinus Thrombosis
- Cell Adhesion Molecules Research
- Hereditary Neurological Disorders
- Muscle metabolism and nutrition
- Myasthenia Gravis and Thymoma
- Heat shock proteins research
- Biotin and Related Studies
- Restless Legs Syndrome Research
- Mesenchymal stem cell research
- Sirtuins and Resveratrol in Medicine
- Spinal Hematomas and Complications
Clinique Saint Pierre
2013-2024
UCLouvain
2001-2014
KU Leuven
2003
Cliniques Universitaires Saint-Luc
1999
We have demonstrated previously that adult human synovial membrane-derived mesenchymal stem cells (hSM-MSCs) myogenic potential in vitro (De Bari, C., F. Dell'Accio, P. Tylzanowski, and F.P. Luyten. 2001. Arthritis Rheum. 44:1928–1942). In the present study, we characterized their differentiation a nude mouse model of skeletal muscle regeneration provide proof principle use for repair mdx Duchenne muscular dystrophy. When implanted into regenerating muscle, hSM-MSCs contributed to myofibers...
Abstract Background Evidence of immune-mediated neurological syndromes associated with the severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection is limited. We therefore investigated clinical, serological and CSF features disease 2019 (COVID-19) patients manifestations. Methods Consecutive COVID-19 manifestations other than isolated anosmia and/or non-severe headache, no previous or psychiatric disorders were prospectively included. Neurological examination was performed in...
High-fat diets are known to decrease muscle protein synthesis, the adaptation overload, and insulin sensitivity. Conditions that disrupt endoplasmic reticulum (ER) homeostasis lead activation of unfolded response (UPR) is associated with decreases in chronic inflammation, resistance. The purpose present study was establish whether ER stress induced by a high-fat diet skeletal can mTORC1 activity synthesis cells. Two independent protocols feeding activated UPR mice. In first study, mice...
Duchenne muscular dystrophy results from the absence of dystrophin, a cytoskeletal protein. Previously, we have shown in transgenic mouse model disease (mdx) that high levels expression dystrophin-related protein, utrophin can prevent pathology. We developed new where muscle specific was conditioned by addition tetracycline water. Transgene turned on at different time points: utero, birth, 10 and 30 days after birth. obtained moderate expression, variable fibre to (mosaicism) but sufficient...
In this study, we compared the effect of sprint interval training (SIT) in normoxia versus hypoxia on muscle glycolytic and oxidative capacity, monocarboxylate transporter content, endurance exercise performance.Healthy male volunteers (18-30 yr) performed 6 wk SIT a cycling ergometer (30-s sprints vs 4.5-min rest intervals; 3 d · wk(-1)) either normobaric (HYP, FiO2 = 14.4%, n 10) or (NOR, 20.9%, 9). The control group did not train (CON, 10). Training load was increased from four per...
This study investigated whether a 200-km run modulates signaling pathways implicated in cellular stress skeletal muscle, with special attention paid to the endoplasmic reticulum (ER) and activation of ubiquitin-proteasome pathway.Eight men ran 200 km (28 h 03 min ± 2 01 min). Two muscle biopsies were obtained from vastus lateralis wk before 3 after race. Mitogen-activated protein kinase, pathway, ER stress, inflammation, oxidative markers assayed by Western blot analysis or quantitative...
Toll-like receptors 2 and 4 (TLR2, TLR4) are found in the membrane of skeletal muscle cells. A variety molecular components can activate TLR2 TLR4, among others, long-chain fatty acids. The subsequent downstream signaling triggers mitogen-activated protein kinase (MAPK) nuclear factor-κB (NF-κB) pathways. Therefore, purpose this study was to test whether an elevation extracellular nonesterified acids (NEFA) observed during endurance exercise may MAPK NF-κB pathways via TLR4.tlr2 tlr4 mice...
We report a patient with clinical history and neurologic examination consistent acute stroke. Diffusion-weighted fast fluid-attenuated inversion recovery MRI obtained 4 hours after stroke onset detected focal abnormalities suggestive of ischemic brain damage. The deficit the imaging both resolved completely at follow-up. This illustrates complete resolution early changes observed diffusion-weighted hyperacute phase in TIA.
Abstract Introduction : Our goal was to determine whether in vivo administration of the proteasome inhibitor MG132 can prevent muscle atrophy caused by hindlimb unloading (HU). Methods Twenty‐seven NMRI mice were assigned a weight‐bearing control, 6‐day HU, or HU+MG132 (1 mg/kg/48 h) treatment group. Results Gastrocnemius wasting significantly less (−6.7 ± 2.0%) compared with HU animals (−12.6 1.1%, P = 0.011). also associated an increased expression MuRF‐1 ( 0.006), MAFbx 0.001), and USP28...
1 The effects of tetanus duration on the relaxation rate extensor digitorum longus (EDL) and flexor brevis (FDB) muscles were studied in normal (wild-type, WT) parvalbumin-deficient (PVKO) mice, at 20 °C. 2 In EDL PVKO, was low unaffected by (< 3.2 s). contrast, WT decreased when increased from 0.2 to s. muscles, fast recovered as rest interval increased. 3 Specific effect parvalbumin asserted calculating difference between PVKO muscles. For EDL, constant slowing 1.10 s−1 tetanization;...
ABSTRACT Introduction : There is uncertainty as to whether the Guillain‐Barré syndrome (GBS) subtypes, acute inflammatory demyelinating polyradiculoneuropathy (AIDP) and motor axonal neuropathy (AMAN), can be diagnosed electrophysiologically. Methods We prospectively included 58 GBS patients. Electrodiagnostic testing (EDX) was performed at means of 5 33 days after disease onset. Two traditional one recent criteria sets were used classify studies or axonal. Results correlated with...
The ubiquitin-proteasome pathway (UPP) is a major protein degradation that activated during sepsis and has been proposed as therapeutic target for preventing skeletal muscle loss due to cachexia. Although several studies have investigated the modulation of proteasome activity in response LPS administration, none characterized overall UPP administration fate inhibition.Here, we determined pattern main key components gastrocnemius (GAS) mice acute phase lipopolysaccharide (LPS)-mediated...
Abstract Defects in human calpain 3 are responsible for limb‐girdle muscular dystrophy type 2A, an autosomal‐recessive disorder characterized mainly by late‐onset proximal atrophy. A corresponding murine model has previously been generated gene targeting. In this report, activity of 3–deficient (capn3 −/− ) mice was evaluated at different ages. Growth curves showed a progressive global Histological examination throughout the lifespan confirmed dystrophic lesions. Whole animal tests only mild...
Background. Transcranial direct current stimulation (tDCS) has been suggested to improve poststroke recovery. However, its effects on bimanual motor learning after stroke have not previously explored. Objective. We investigated whether dual-tDCS of the primary cortex (M1), with cathodal and anodal tDCS applied over undamaged damaged hemispheres, respectively, improves retention a new cooperative skill in patients. Method. Twenty-one chronic hemiparetic patients were recruited for randomized,...
Abstract Dystrophic mice ( mdx ) and their controls (C57/Bl10) were fed for 1 month with a diet or without creatine (Cr) enrichment. Cr supplementation reduced mass (by 19%, P < 0.01) mean fiber surface 25%, 0.05) of fast‐twitch muscles. In both strains, tetanic tension increased slightly (9.2%) reaching statistical significance = 0.08), relaxation time by 16% 0.001). However, had no protective effect on the other hallmarks dystrophy such as susceptibility to eccentric contractions; large...
Mitogen-activated protein kinase (MAPK) pathways are activated in skeletal muscle during endurance exercise, but the upstream molecular events incompletely resolved. As an increase plasma nonesterified fatty acids (NEFA) is a common feature of long-lasting authors tested hypothesis that NEFA contribute to activation MAPK exercise. Acipimox was used before and exercise prevent elevation levels healthy subjects patients with diabetes. In 2 separate studies, cycled for hr diabetes 1 at 50% W...
Toll-like receptors 2 (TLR2) and 4 (TLR4) are present in the plasma membrane of skeletal muscle cells where their functions remain incompletely resolved. They can bind various extracellular ligands, such as FSL-1, lipopolysaccharide (LPS) and/or palmitic acid (PA). We have investigated link between PA, TLR2/4 ribosomal S6 kinase 1 (S6K1) C2C12 myotubes. Incubation with agonists either TLR2 or TLR4, a high concentration increased S6K1 phosphorylation. Canonical upstream kinases S6K1, protein...