Surface enhanced Raman spectroscopy (SERS) is a powerful vibrational technique that allows for highly sensitive structural detection of low concentration analytes through the amplification electromagnetic fields generated by excitation localized surface plasmons. SERS has progressed from studies model systems on roughened electrodes to sophisticated studies, such as single molecule spectroscopy. We summarize current state knowledge concerning mechanism and new substrate materials. highlight...

Determining the existence of any direct spectral relationship between far-field scattering properties and near-field Raman-enhancing surface-enhanced Raman spectroscopy (SERS) substrates has been a challenging task with only few significant results to date. Here, we prove that hot spot dominated systems show little dependence on because differences near- localized surface plasmon resonance (LSPR) effects as well excitation new modes via emitter. We directly probe light interactions using...

This perspective presents recent developments in the application of surface-enhanced Raman spectroscopy (SERS) to biosensing, with a focus on vivo diagnostics. We describe concepts and methodologies developed date target analytes that can be detected. also discuss how SERS has evolved from "point-and-shoot" stand-alone technique an analytical chemistry laboratory integrated quantitative tool for multimodal imaging Finally, we offer guide future context clinical

Interest in nanotechnology is driven by the unique and novel properties of nanoscale materials such as strong interaction metal particles with light, caused localized surface plasmon resonances (LSPRs). In this perspective article we review discuss prominent advantages advances single particle studies plasmonic nanostructures. Common techniques recent improvements spatial spectral resolution will first be outlined, covering both far-field near-field phenomena. Then, new insight information...

This paper demonstrates the direct sensing of glucose at physiologically relevant concentrations with surface-enhanced Raman spectroscopy (SERS) on gold film-over-nanosphere (AuFON) substrates functionalized bisboronic acid receptors. The combination selectivity in receptor and spectral resolution SERS data allow sensors to resolve high backgrounds fructose and, multivariate statistical analysis, detect accurately 1–10 mM range. Computational modeling supports assignments normal modes...

We report here the first fabrication of aluminum film-over nanosphere (AlFON) substrates for UV surface-enhanced resonance Raman scattering (UVSERRS) at deepest wavelength used to date (λex = 229 nm). characterize AlFONs fabricated with two different support microsphere sizes using localized surface plasmon spectroscopy, electron microscopy, SERRS adenine, tris(bipyridine)ruthenium(II), and trans-1,2-bis(4-pyridyl)-ethylene, SERS 6-mercapto-1-hexanol (as a nonresonant molecule), dielectric...

Surface-enhanced spatially offset Raman spectroscopy (SESORS) is a label-free vibrational that has the potential for in vivo imaging. Previous SESORS experiments have been limited to acquiring spectra using SERS substrates implanted under skin or from nanoparticles embedded tissue. Here we present measurements of active coated with reporter molecule (nanotags) acquired, first time, through bone. We demonstrate ability measure various thicknesses (3-8 mm) also show diluted nanotag samples (~2...

The development of a sensor for the rapid and sensitive detection neurotransmitters could provide pathway diagnosis neurological diseases, leading to discovery more effective treatment methods. We investigate use surface enhanced Raman spectroscopy (SERS) based sensors melatonin, serotonin, glutamate, dopamine, GABA, norepinephrine, epinephrine. Previous studies have demonstrated SERS neurotransmitters; however, there has been no comprehensive study on effect metal used as substrate or...

The ability to noninvasively detect neurotransmitters through the skull would aid in understanding brain function and development of neurological diseases. Surface enhanced spatially offset Raman spectroscopy (SESORS) is a powerful technique that combines sensitivity surface-enhanced (SERS) with (SORS) probe subsurface layers. Here we present SERS measurements (melatonin, serotonin, epinephrine) at various concentrations followed by SESORS concentration as low 100 μM tissue mimic cat skull....

Cortisol is an important steroid hormone in human physiology. Variations or abnormalities the physiological cortisol levels control acute and chronic stress response, as well contribute to diseases syndromes including Addison's disease Cushing syndrome. The ability monitor range key diagnosis monitoring of these conditions, where current methodology for determination relies on instrumentation that requires extensive sample preparation, long run times, destructive sample. Raman spectroscopy...

Detection techniques for neurotransmitters that are rapid, label-free, and non-invasive needed to move towards earlier diagnosis of neurological disease.

Abstract Background: The nature of voice as forensic evidence holds immense value. examination is highly influenced by various factors such recording devices, environmental factors, and individual health conditions beyond the norm. rapid advancement technology has led to evolution numerous devices including tape-based recorders, reel-to-reel-based analog tape recorders which have transitioned into digital media. widespread accessibility mobile phone be it Android, iOS, or Feature phone,...

Rapid and accurate detection of glutathione in its reduced (GSH) oxidized (GSSG) forms is essential for monitoring oxidative stress biological systems. Oxidative a key indicator of...

Abstract The complex of biofactors secreted by mesenchymal stem cells, termed the secretome, can promote wound healing. Studies using this secretome often utilise material collected from short term and sub‐confluent lab‐scale cultures. Secretome was derived prolonged culture high‐density industrial scale human Wharton's jelly cells its effects on healing assessed. In vitro cell proliferation scratch closure assays showed that treatment dose‐dependently increased promoted closure....

We constructed an ultrahigh-throughput, high-resolution ultraviolet (UV) Raman spectrograph that utilizes a high-efficiency filter-stage monochromator and high-dispersion Echelle spectrograph. The total of six mirrors two gratings, with overall efficiency at 229 nm ~18%. limiting resolution our spectrometer is 0.6 cm⁻¹ full width half-maximum (FWHM), as measured for Rayleigh scattering. Use 1 mm-wide entrance slit gives rise to approximately 10 FWHM nm. ultrahigh throughput enables...

Here, Raman spectroscopy is used to develop a univariate partial least squares (PLS) calibration capable of quantifying geochemistry in synthetic and natural silicate glass samples. The yields eight oxide-specific models that allow predictions silicon dioxide (SiO 2 ), sodium oxide (Na O), potassium (K calcium (CaO), titanium (TiO aluminum (Al O 3 ferrous (FeO T magnesium (MgO) (wt%) glasses spanning wide range compositions, while also providing correlation-coefficient matrices highlight the...

Recently, we have shown that autocrine transforming growth factor-alpha (TGF-alpha) controls the expression of integrin alpha2, cell adhesion to collagen IV and motility in highly progressed HCT116 colon cancer cells (Sawhney, R. S., Zhou, G-H. K., Humphrey, L. E., Ghosh, P., Kreisberg, J. I., Brattain, M. G. (2002) Biol. Chem. 277, 75-86). We now report basal alpha2 its biological effects are controlled by constitutive activation extracellular signal-regulated/mitogen-activated protein...