A5044818017- Mosquito-borne diseases and control
- interferon and immune responses
- Viral Infections and Vectors
- RNA modifications and cancer
- Malaria Research and Control
- Virology and Viral Diseases
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Inflammasome and immune disorders
- Dengue and Mosquito Control Research
- Immune Response and Inflammation
- Global Public Health Policies and Epidemiology
- Viral Infections and Outbreaks Research
- Cytokine Signaling Pathways and Interactions
- Vector-Borne Animal Diseases
Medical Research Council
2018-2025
University of California, Berkeley
2020-2025
MRC Human Immunology Unit
2018-2021
University of Oxford
2018-2021
Instituto Evandro Chagas
2013
Abstract Zika virus (ZIKV) is a major public health concern in the Americas. We report that ZIKV infection and RNA extracted from infected cells potently activated induction of type I interferons (IFNs). This effect was fully dependent on mitochondrial antiviral signaling protein (MAVS), implicating RIG‐I‐like receptors (RLRs) as upstream sensors viral RNA. Indeed, RIG‐I related sensor MDA5 contributed to IFN response cells. found NS5 recent Brazilian isolate blocked downstream RLRs also...
Preexisting cross-reactive antibodies have been implicated in both protection and pathogenesis during subsequent infections with different dengue virus (DENV) serotypes (DENV1-4). Nonetheless, humoral immune correlates mechanisms of remained elusive. Using a systems serology approach to evaluate responses, we profiled plasma collected before inapparent or symptomatic secondary DENV3 infection from our pediatric cohort Nicaragua. Children protected had more anti-envelope (E)...
The four dengue virus serotypes (DENV1-4) and the related Zika flavivirus (ZIKV) are major public health concerns worldwide. Primary immunity against ZIKV increases risk of a subsequent severe DENV2 infection, presenting significant challenge for developing safe effective vaccines. However, mechanisms driving this phenomenon remain unclear. Leveraging our long-standing Pediatric Dengue Cohort Study in Nicaragua, we show that serum anti-NS1 IgA antibodies elicited after primary infection...
<title>Abstract</title> MDA5 is an innate immune RNA sensor that senses infection with a range of viruses and other pathogens. MDA5’s agonists are not well defined. We used single-nucleotide resolution crosslinking immunoprecipitation (iCLIP) to study its ligands. Surprisingly, upon SARS-CoV-2 or encephalomyocarditis virus, bound overwhelmingly cellular RNAs. Many binding sites were intronic proximal <italic>Alu</italic> elements potentially base-paired structures. Concomitantly, cytoplasmic...
Hypoxia is a common phenomenon in solid tumours strongly linked to the hallmarks of cancer. promotes local immunosuppression and downregulates type I interferon (IFN) expression signalling, which contribute success many cancer therapies. Double-stranded RNA (dsRNA), transiently generated during mitochondrial transcription, endogenously activates IFN pathway. We report effects hypoxia on generation dsRNA (mtdsRNA) breast found significant decrease production different cell lines under...
Primary infection with one of four dengue virus serotypes (DENV1-4) may generate antibodies that protect or enhance subsequent secondary heterotypic infections. However, the characteristics cross-reactive associated protection from symptomatic and severe disease are not well-defined. We selected plasma samples collected before a DENV was classified either as fever (DF, n = 31) hemorrhagic fever/dengue shock syndrome (DHF/DSS, 33) our longstanding pediatric cohort in Nicaragua. screened...
ABSTRACT Hypoxia is a common phenomenon in solid tumours strongly linked to the hallmarks of cancer. promotes local immunosuppression and downregulates type I interferon (IFN) expression signalling, which contribute success many cancer therapies. Double-stranded RNA (dsRNA), transiently generated during mitochondrial transcription, endogenously activates IFN pathway. We report effects hypoxia on generation dsRNA (mtdsRNA) breast found significant decrease production different cell lines...
Os arbovírus estão amplamente distribuídos pelo mundo e, no Brasil, cerca de 200 espécies diferentes têm sido isoladas e muitas delas associadas a doenças em humanos. A maioria dos está classificada na família Bunyaviridae que, por possuírem três segmentos RNA, apresentam uma característica importante rearranjo genético, formando novos vírus mundo. O advento novas ferramentas biologia molecular para sequenciamento genômico, aliado aos clássicos testes sorológicos fixação do complemento (FC),...
The ‘2019 Research Capacity Network (REDe) workshop series’ was an initiative led by Brazil-based REDe coordinators and Global Health (TGHN) in partnership with Brazilian researchers interested arboviruses. This has provided crucial training to the local research community offering transferable skills effectively respond health emergencies, impact beyond arboviral diseases, as evidenced further activities undertaken during COVID-19 pandemic. success of this approach resulted from several...