A5101401422- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Cancer Genomics and Diagnostics
- Nematode management and characterization studies
- Soybean genetics and cultivation
- Cancer-related Molecular Pathways
- Advanced Breast Cancer Therapies
- Agronomic Practices and Intercropping Systems
- Genetics, Bioinformatics, and Biomedical Research
- Plant-Microbe Interactions and Immunity
- Advanced Electron Microscopy Techniques and Applications
- HIV Research and Treatment
- Bioinformatics and Genomic Networks
- Heat shock proteins research
- Enzyme Production and Characterization
- Enzyme Structure and Function
- thermodynamics and calorimetric analyses
- PARP inhibition in cancer therapy
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Phytase and its Applications
- RNA Research and Splicing
Xinyang Normal University
2016-2025
Fujian Agriculture and Forestry University
2020-2023
University of Pittsburgh
2011-2014
Huazhong Agricultural University
2012-2013
State Key Laboratory of Plant Genomics
2012
Institute of Genetics and Developmental Biology
2012
University of Idaho
2012
Tsinghua University
2001-2012
Memorial Sloan Kettering Cancer Center
2006
Cornell University
2006
Multiple types of degradative enzymes, including cathepsins the cysteine protease family, have been implicated in regulation angiogenesis and invasion during cancer progression. Several are up-regulated a mouse model pancreatic islet cell carcinogenesis (RIP1-Tag2), tumor progression is impaired following their collective pharmacologic inhibition. Using null mutations four cathepsins, we now dissected individual roles development. Mutants B or S formation angiogenesis, while cathepsin L...
The symbiosis receptor kinase, SymRK, is required for root nodule development. A SymRK-interacting protein (SIP2) was found to form complex with SymRK in vitro and planta. interaction between SIP2 conserved legumes. gene expressed all Lotus japonicus tissues examined. represents a typical plant mitogen-activated kinase (MAPKK) exhibited autophosphorylation transphosphorylation activities. Recombinant could phosphorylate casein the Arabidopsis thaliana MAP MPK6. not use one another as...
Abstract Nod Factor Receptor5 (NFR5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. It forms a heterodimer with NFR1 and required for early plant responses to Rhizobium infection. A Rho-like small GTPase from Lotus japonicus was identified as NFR5-interacting protein. The amino acid sequence of this closest Arabidopsis (Arabidopsis thaliana) ROP6 Medicago truncatula designated LjROP6. interaction between Rop6 NFR5 occurred both vitro planta. No...
TRIM proteins play important roles in the innate immune defense against retroviral infection, including human immunodeficiency virus type-1 (HIV-1). Rhesus macaque TRIM5α (TRIM5αrh) targets HIV-1 capsid and blocks infection at an early post-entry stage, prior to reverse transcription. Studies have shown that binding of assembled is essential for restriction requires coiled-coil B30.2/SPRY domains, but molecular mechanism not fully understood. In this study, we investigated, by cryoEM...
Amino acid transporters (AATs) play important roles in transporting amino across cellular membranes and are essential for plant growth development. To date, the AAT gene family soybean (Glycine max L.) has not been characterized. In this study, we identified 189 genes from entire genomic sequence, classified them into 12 distinct subfamilies based upon their sequence composition phylogenetic positions. further investigate functions of these genes, analyzed chromosome distributions,...
Root nodule symbiosis (RNS) is one of the most productive and economical systems for nitrogen fixation, previous studies have shown that several nodule-specific C2H2-zinc finger proteins (ZFPs) play important roles in establishment function. However, C2H2-ZFPs are widespread ZFPs eukaryotes, a great variation structure function exist among family members. It remains largely unclear whether or not special types C2H2-ZF genes participate symbiosis, especially soybean. In present study, we...
Abstract The symbiosis receptor kinase (SymRK) is required for morphological changes of legume root hairs triggered by rhizobial infection. How protein turnover SymRK regulated and how the nodulation factor signals are transduced downstream not known. In this report, a SymRK-interacting E3 ubiquitin ligase (SIE3) was shown to bind ubiquitinate SymRK. SIE3-SymRK interaction ubiquitination were occur in vitro planta. SIE3 represents new class plant-specific ligases that contain unique pattern...
Histone modifications, such as methylation and demethylation, play an important role in regulating chromatin structure gene expression. The JmjC domain-containing proteins, family of histone lysine demethylases (KDMs), a key maintaining homeostasis vivo. In this study, we performed comprehensive analysis the jumonji C (JmjC) soybean genome identified 48 genes (GmJMJs) distributed unevenly across 18 chromosomes. Phylogenetic showed that these can be divided into eight groups. GmJMJs within...
Summary Focused ion beam milling at cryogenic temperatures (cryo‐FIB) is a valuable tool that can be used to thin vitreous biological specimens for subsequent imaging and analysis by cryo‐transmission electron microscopy (cryo‐TEM) in frozen‐hydrated state. This technique offers the potential benefit of eliminating mechanical artefacts are typically found with cryo‐ultramicrotomy. However, due additional complexity transferring samples out FIB, contamination devitrification amorphous ice...
During retrovirus particle maturation, the assembled Gag polyprotein is cleaved by viral protease into matrix (MA), capsid (CA), and nucleocapsid (NC) proteins. To form mature capsid, CA rearranges, resulting in a lattice composed of hexameric pentameric units. Recent structural studies HIV-1 revealed several inter-subunit interfaces lattice, including three-fold interhexamer interface that critical for proper stability. Although general architecture immature particles has been provided...
Abstract Soybean ( Glycine max L.) is an important oil, food and economic crop in the world. High salinity severely affects growth yield of soybean. Overexpressing a specific anti‐retroviral transcription factor by biotechnology effective way to cultivate new stress‐tolerant varieties TGA subfamily bZIP plays role abiotic stress responses. A gene GmTGA13 was cloned expression, subcellular localization transcriptional activity were measured. Through Ag. tumefaciens mediated flower dip method...
The balanced regulation of innate immunity plays essential roles in rhizobial infection and the establishment maintenance symbiosis. evolutionarily conserved cell death suppressor Bax inhibitor-1 dual nodule symbiosis, providing a valuable clue balancing while it remains largely unexplored legume Lotus japonicus. In present report, BI-1 gene family L. japonicus was identified characterized. We 6 genes that translate into peptides containing 240-255 amino acids with different structural...
Summary Transcription factor complex formation is a central step in regulating gene expression. In this report, novel MYB coiled‐coil transcription referred to as IPN 2, for I nteracting P rotein of NSP described. The interaction between 2 and was examined by protein pull‐down assays bimolecular fluorescence complementation ( B i FC ). Subcellular localization proteins, expression function were assessed transgenic hairy roots expressing tagged recombinant promoter‐reporter RNA interference...
Abstract The gene networks surrounding Nod factor receptors that govern the symbiotic process between legumes and rhizobia remain largely unexplored. Here, we identify 13 novel GmNFR1α-associated proteins by yeast two-hybrid screening, describe a potential interacting protein, GmBI-1α. GmBI-1α had highest positive correlation with GmNFR1α in co-expression network analysis, its expression at mRNA level roots was enhanced rhizobial infection. Moreover, GmBI-1α–GmNFR1α interaction shown to...
Plant papain-like cysteine proteases (PLCPs) are a large class of proteolytic enzymes and play important roles in root nodule symbiosis (RNS), while the whole-genome studies PLCP family genes legume quite limited, Glycine max PLCPs (GmPLCPs) nodulation, development senescence not fully understood.In present study, we identified 97 GmPLCPs performed genome-wide survey to explore expansion soybean their relationships RNS. Nineteen paralogous pairs genomic segments, consisting 77 GmPLCPs,...
Increasing evidence suggests that Rho of plant (ROP) GTPases play important roles in the rhizobium–legume symbiotic nodulation, but molecular mechanisms their regulation symbiosis remain poorly understood. In this study, we showed ROP4 Lotus japonicus (LjROP4) is involved interaction between L. and Mesorhizobium loti. Tissue expression analysis LjROP4 expressed highly root. Histochemical staining after rhizobia inoculation, GUS reporter activity increased root vascular bundle, tip, lateral...