A5069934698- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Telomeres, Telomerase, and Senescence
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Carcinogens and Genotoxicity Assessment
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Cell death mechanisms and regulation
- Cell Image Analysis Techniques
- Advanced Proteomics Techniques and Applications
- Circular RNAs in diseases
- BRCA gene mutations in cancer
- Cancer Mechanisms and Therapy
- Advanced Breast Cancer Therapies
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
Soochow University
2021-2024
Rutgers, The State University of New Jersey
2020-2021
Rutgers Cancer Institute of New Jersey
2020-2021
Johnson University
2020
Institute of Neurobiology
2020
Institute of Modern Physics
2012-2018
Chinese Academy of Sciences
2012-2018
University of Chinese Academy of Sciences
2012-2014
Beijing Administration Institute
2012-2014
Lanzhou University
2012
Abstract The BRCA2 tumor suppressor protects genome integrity by promoting homologous recombination-based repair of DNA breaks, stability stalled replication forks and damage-induced cell cycle checkpoints. deficient cells display the radio-resistant synthesis (RDS) phenotype, however mechanism has remained elusive. Here we show that without are unable to sufficiently restrain fork progression after damage, underrestrained is due primarily Primase-Polymerase (PRIMPOL)-mediated repriming...
Ferroptosis is an iron-dependent regulated form of cell death implicated in various diseases, including cancers, with its progression influenced by peroxidation phospholipids and dysregulation the redox system. Whereas extracellular matrix tumors provides mechanical cues influencing tumor initiation progression, impact on ferroptosis mechanisms remains largely unexplored. In this study, we reveal that heightened tension sensitizes cells to ferroptosis, whereas decreased mechanics confers...
The mechanisms of radiation-induced bystander effects (RIBE) have been investigated intensively over the past two decades. Although quite a few reports demonstrated that cytokines such as TGF-β1 are induced within directly irradiated cells and play critical roles in mediating effects, little is known about signaling pathways occur cells. crucial question to why RIBE signals cannot be infinitely transmitted, therefore, remains unclear. In present study, we showed miR-663, radiosensitive...
Persistent DNA damage is considered as a main cause of cellular senescence induced by ionizing radiation. However, the molecular bases and their contribution to are not completely clear. In this study, we found that both heavy ions X-rays in human uveal melanoma 92–1 cells. By measuring associated-β-galactosidase cell proliferation, identified were more effective at inducing than X-rays. We observed less efficient repair when was compared with most irreparable complex single strand breaks...
Diploid cells undergoing senescence and mitotic slippage have been reported in the literature. However, mechanisms triggering long-term G2-arrested are currently unclear. Previously, we that cell cycle of human uveal melanoma line, 92-1, is suspended for up to 6 d upon exposure 10 Gy ionizing radiation (IR), followed by senescence. In current study, initially distinguished blocked 92-1 from confirming blockage G2 phase. We subsequently showed genes essential G2-M transition prematurely...
Ribosome biogenesis is a fundamental process required for cell proliferation. Although evolutionally conserved, the mammalian ribosome assembly system more complex than in yeasts. BCCIP was originally identified as BRCA2 and p21 interacting protein. A partial loss of function sufficient to trigger genomic instability tumorigenesis. However, complete deletion arrested growth lethal mice. Here, we report that fraction localizes nucleolus regulates 60S biogenesis. Both abrogation nucleolar...
Cell cycle checkpoint is a self-protective mechanism for cells to monitor genome integrity and ensure the high-fidelity transmission of genetic information daughter cells. Insufficient function cell checkpoints has been demonstrated partially account tumor initiation, promotion progression. In ten melanoma lines that we tested in preliminary experiments, two human uveal lines, 92-1 OCM-1, were found be significantly different terms radiosensitivity but similar DNA repair ability. Evident G 2...
Alterations in microRNA (miRNA) expression have been observed cells subjected to exogenous stresses, implying that miRNAs play an important role cellular stress response; however, the underlying mechanism is still largely unknown. In present study, we found miR-3928 was implicated response ionizing radiation. After exposed X-rays, increased 1.5 h and then decreased, meanwhile Dicer, a key component miRNA processing machinery, gradually. An oscillation of both mature Dicer mRNA from 2 3.5...
The occurrence of distant tumor metastases is a major barrier in non-small cell lung cancer (NSCLC) therapy, and seriously affects clinical treatment patient prognosis. Recently, long non-coding RNAs (lncRNAs) have been demonstrated to be crucial regulators metastasis cancer. aim this study was reveal the underlying mechanisms novel lncRNA LNC CRYBG3 regulating NSCLC metastasis. Experimental results showed that upregulated cells compared with normal tissue cells, its level involved these...
Abstract Background. Carbon ion therapy may be better against cancer than the effects of a photon beam. To investigate biological advantage carbon beam over X-rays, radioresistant cell line HeLa cells were used. Radiationinduced changes in processes investigated post-irradiation at 1 h by clinically relevant radiation dose (2 Gy X-ray and 2 beam). The differential expression proteins collected for analysing effects. Materials methods. Hela In our study, stable isotope labelling with amino...
Melanoma is a malignant tumor with high invasive and metastatic properties. Though radiation the major therapy for melanoma, its radio-resistance has been shown to severely influence clinical outcome. So it imperative enhance sensitivity of uveal melanoma cells radiotherapy. Previously, we found that cell cycle 92-1 was suspended remained unchanged up 5 days after exposure 10 Gy X-rays, which might be relevant radio-sensitivity cells. To further investigate suspension-associated proteins,...
BCCIP was originally identified as a BRCA2 and CDKN1A/p21 interaction protein. Although partial loss of function is sufficient to trigger genomic instability tumorigenesis, complete deletion lethal cells. Using Rosa26-CreERT2 mouse models, we found that induced Bccip in adult mice caused an acute intestinal epithelial denudation cannot be relieved by co-deletion Trp53. The critical role intestine renewal verified with Villin-CreERT2 model. epithelium degeneration associated rapid the...
Cancer cells maintain a high level of aerobic glycolysis (the Warburg effect), which is associated with their rapid proliferation. Many studies have reported that the suppression and activation oxidative phosphorylation can repress growth cancer through regulation key regulators. Whether effect could be switched by some other environmental stimulus? Herein, we report an interesting phenomenon in alternated between mitochondrial respiration depending on type radiation they were exposed to. We...
Objective To establish a model to predict the cell-cycle process in response ionizing radiation. Methods Human choroidal malignant melanoma 92-1 cells were used and cell cycle distribution of was analyzed 0-96 h after exposure X-rays. A phenomenological constructed based on biological knowledge describe dynamics experiments. Results In present study, cellcycle synchronized responding various doses radiation. The simulation results obtained with consistent experimental data,...
The dynamic behaviors of microRNA and mRNA under external stress are studied with biological experiments mathematics models. In this study, we developed a mathematic model to describe the phenomenon for first time reported that, as responses stress, expression levels sustained oscillation. And period oscillation is much shorter than several transcriptional regulation negative feedback loop.
Objective To explore the functions of DNA-PKcs in cellular low dose hyper-radiosensitivity. Methods Colony-formation assay was used to detect survival fractions M059K and M059J cell lines treated by X-ray irradiation. Micronucleus γ-H2AX foci were measure radiation-induced DNA damage. Western blot relative expression levels phospho-Chk1, total Chk1, phospho-Chk2 Chk2 cells. Results The hyper-radiosensitivity observed cells irradiated with doses lower than 1 Gy. damage did not...