A5103063395- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Traumatic Brain Injury and Neurovascular Disturbances
- Acute Ischemic Stroke Management
- Heme Oxygenase-1 and Carbon Monoxide
- S100 Proteins and Annexins
- Traumatic Brain Injury Research
- Inflammasome and immune disorders
- Vagus Nerve Stimulation Research
- Aortic Disease and Treatment Approaches
- Immune Response and Inflammation
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Respiratory Support and Mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Anesthesia and Neurotoxicity Research
- Spine and Intervertebral Disc Pathology
- Heat shock proteins research
- Cerebrovascular and Carotid Artery Diseases
- Intracerebral and Subarachnoid Hemorrhage Research
- Autophagy in Disease and Therapy
- Tendon Structure and Treatment
- Spinal Fractures and Fixation Techniques
Zhongshan Hospital
2011-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
Army Medical University
2015-2024
Daping Hospital
2015-2024
Beijing Luhe Hospital Affiliated to Capital Medical University
2024
New York Medical College
2023
Army Medical College
2023
Chinese Academy of Agricultural Sciences
2023
Dalian Medical University
2023
Sheng Jing Hospital
2023
During brain injury, extracellular adenosine and glutamate levels increase rapidly dramatically. We hypothesized that local in the dictates adenosine–adenosine A 2A receptor (A R) effects on neuroinflammation damage outcome. Here, we showed that, presence of low concentrations glutamate, R agonist 3-[4-[2-[[6-amino-9-[(2 ,3 ,4 S ,5 )-5-(ethylcarbamoyl)-3,4-dihydroxy-oxolan-2-yl]purin-2-yl]amino]ethyl]phenyl]propanoic acid (CGS21680) inhibited lipopolysaccharide (LPS)-induced nitric oxide...
Abstract Recent studies have shown that after traumatic brain injury (TBI), the number of autophagosomes is markedly increased in cells surrounding wound; however, whether autophagy enhanced or suppressed by TBI remains controversial. In our study, we used a controlled cortical impact system to establish models mild, moderate and severe TBI. mild model, levels autophagy-related protein 6 (Beclin1) 12 (ATG12)-autophagy-related 5 (ATG5) conjugates were increased, indicating initiation...
Cognitive impairment in the late stage of traumatic brain injury (TBI) is associated with NOD-, LRR and pyrin domain-containing protein 3 (NLRP3) inflammasome, which plays an important role neuroinflammation. Although classical inflammatory pathways have been well-documented TBI (4-8 weeks post-injury), mechanism by NLRP3 inflammasome impairs cognition still unclear.Mice lacking gene encoding for (NLRP3-knockout mice) their wild-type littermates were used a controlled cortical impact model...
Traumatic brain injury (TBI) is a serious health threat worldwide, especially for the younger demographic. Our previous study demonstrated that HET0016 (a specific inhibitor of 20-hydroxyeicosatetraenoic acid synthesis) can decrease lesion volume in immature post-TBI; however, its mechanism action and association with pyroptosis post-TBI are unclear. In this study, we established controlled cortical impact (CCI) rat model (postnatal day 9–10) observed increased expression indicators...
The bone marrow-derived cell (BMDC)-associated inflammatory response plays a key role in the development of acute lung injury (ALI). Activation adenosine A2A receptor (A2AR) is generally considered to be antiinflammatory, inhibiting BMDC activities protect against ALI. However, present study, we found that mouse model neurogenic ALI induced by severe traumatic brain (TBI), A2AR exerted proinflammatory effect, aggravating damage. This contrast antiinflammatory effect observed oleic...
Abstract Systemic inflammatory response syndrome (SIRS) is an overwhelming whole body inflammation caused by infectious diseases or sterile insults. Neutrophils are the dominant participants during inflammation, and their survival death determine initiation as well resolution of SIRS. Apoptosis autophagy two fundamental cellular processes that modulating cell fate, but correlation regulators in neutrophils under SIRS condition have not been elucidated. In this study, we demonstrated high...
Abstract Tau is a microtubule-associated protein, and the oligomeric hyperphosphorylated forms of tau are increased significantly after neurotrauma considered important factors in mediating cognitive dysfunction. Blockade adenosine A 2A receptors, either by caffeine or gene knockout (KO), alleviates dysfunction traumatic brain injury (TBI). We postulated that R activation exacerbates impairment via promoting hyperphosphorylation. Using mouse model moderate controlled cortical impact, we...
Colorectal cancer (CRC) is one of the leading causes cancer-related deaths around world. Recently, using high-throughput techniques, long non-coding RNAs (lncRNAs) have been shown to play an important role in CRC progression. In present study, we aimed determine lncRNA DLX6 Antisense RNA 1 (DLX6-AS1) tissues and cell lines investigate molecular mechanisms DLX6-AS1 progression.Quantitative real-time PCR was performed detect gene expression; counting kit-8, colony formation, invasion,...
We recently demonstrated that Ski is a novel wound healing-related factor promotes fibroblast proliferation and inhibits collagen secretion. Here, we show increasing local expression by gene transfer not only significantly accelerated healing relieving inflammation, accelerating re-epithelialization formation of granulation tissue, but also reduced scar decreasing production in rat dermal wounds. Similarly, ski healing, the protuberant height volume scars increased maturity hypertrophic...
Traumatic brain injury (TBI), particularly explosive blast-induced TBI (bTBI), has become the most prevalent among military personnel. The disruption of cognitive function is one serious consequences bTBI because its long-lasting effects prevent survivors fulfilling their active duty and resuming normal civilian life. However, mechanisms are poorly understood there no treatment available. This study investigated adenosine A2A receptor (A2AR) on bTBI-induced deficit, explored underlying...
Oxidative stress is becoming increasingly linked to the pathogenesis of abdominal aortic aneurysms (AAAs). The antioxidant activity flavonoids has attracted attention for their possible role in prevention cardiovascular diseases. purpose this study was determine whether an mechanism involved aneurysm formation inhibitory effect afforded by quercetin. Male C57/BL6 mice received quercetin continuously from 2 weeks prior and 6 following AAA induction with extraluminal CaCl2. Quercetin treatment...
Renal interstitial fibrosis (RIF) is the common pathological process of chronic kidney diseases leading inevitably to renal function deterioration. RIF and its preceding epithelial-mesenchymal transition (EMT) are commonly triggered by an early occurring inflammation. However, effective approach prevent EMT still lacking urgent need. Recently, adenosine A2A receptor (A2AR) emerges as a novel inflammation regulator, therefore manipulation A2AR may suppress such protect against RIF. To test...
Abstract The anti‐inflammatory activity of cirsilineol in vivo condition was assessed by measuring the relative organ weight, lung dry/wet weight ratio, protein concentration, and infiltration inflammatory cells bronchoalveolar lavage fluid. We estimated myeloperoxidase levels cytokines, chemokines, markers to analyze efficacy against lipopolysaccharide (LPS)‐induced inflammation. Furthermore, we quantified gene expression NF k B/IKK signaling molecules cirsilineol‐treated untreated acute...
Abstract Aims Adenosine 2A receptor (A R) is widely expressed in the brain and plays important roles neuroinflammation, nucleotide‐binding oligomerization domain, leucine‐rich repeat, pyrin domain‐containing protein 3 (NLRP3) inflammasome a crucial component of innate immune system while regulation A R on it central nervous (CNS) has not been clarified. Methods The effects microglial NLRP3 assembly activation were investigated wild‐type, R‐ or NLRP3‐knockout primary microglia with...
Traumatic brain injury-induced acute lung injury (TBI-ALI) is a serious complication after for which predictive factors are lacking. In this study, we found significantly elevated blood glutamate concentrations in patients with TBI or multiple peripheral trauma (MPT), and more severe injuries showed higher longer durations of levels. Although the increase amplitude was similar between two groups, duration TBI. There were no significant differences MPT regard to ALI status, but levels TBI-ALI...
Abstract Both brain native inflammatory cells and infiltrated peripheral white blood (WBCs) are primary participants in the damage post-TBI. Metabotropic glutamate receptor 5 (mGluR5) has been reported to regulate microglias astrocytes affect inflammation after TBI, but its effect on modulating WBCs remains unclear. In a mouse moderate TBI model, we found that mGluR5 knockout (KO) significantly reduced neutrophil infiltration cytokine expression at 24 hours post which was accompanied by...