A5089148956- Blood groups and transfusion
- Blood transfusion and management
- Erythrocyte Function and Pathophysiology
- Blood donation and transfusion practices
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Platelet Disorders and Treatments
- Prenatal Screening and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Diabetes and associated disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Parvovirus B19 Infection Studies
- Blood disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- Complement system in diseases
- Venous Thromboembolism Diagnosis and Management
- Acute Kidney Injury Research
- Food Allergy and Anaphylaxis Research
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Renal Diseases and Glomerulopathies
- Fetal and Pediatric Neurological Disorders
- Contact Dermatitis and Allergies
- Blood properties and coagulation
- Inflammatory Biomarkers in Disease Prognosis
Odense University Hospital
2009-2025
Kongsberg Innovasjon (Norway)
2017-2020
Seattle University
2017
Region of Southern Denmark
2016
Aalborg University Hospital
2011
Aarhus University Hospital
2011
Næstved Sygehus
2011
Copenhagen University Hospital
2011
Rigshospitalet
2011
BACKGROUND: A combination of antenatal and postnatal RhD prophylaxis is more effective in reducing D immunization pregnancy than alone. Based on the result from screening for fetal RHD gene, Denmark given only to those D− women who carry a D+ fetus. We present an evaluation first national clinical application screening. STUDY DESIGN AND METHODS: In each five Danish health care regions, blood samples were drawn Gestational Week 25. DNA was extracted maternal plasma analyzed presence gene by...
Prenatal and postnatal RhD prophylaxis reduces the risk of immunization in pregnancies RhD-negative women. Based on result from prenatal screening for fetal RHD gene, Denmark is targeted to women who carry an RhD-positive fetus. Here, we present a 2-year evaluation nationwide screening.Blood samples were drawn gestational week 25. DNA was extracted maternal plasma analyzed gene. The results compared with serological typing newborns 12,668 pregnancies. Early compliance assessed 690...
ObjectivesEvaluate six commercial serological assays for detection of IgA, IgM or IgG SARS-CoV-2 antibodies in different disease severities.MethodsThree lateral flow tests (LFTs) (Acro IgM/IgG, CTK Livzon IgM/IgG) and three ELISA (Euroimmun IgA IgG, Wantai IgM) were included. Application was evaluated using samples from 57 patients with a positive reverse transcription polymerase chain reaction, stratified according to severity. Specificity assessed historical 200 blood donors.ResultsWhile...
Results from previous studies regarding platelet function in liver cirrhosis are discordant. The aim was to investigate activation and aggregation patients with alcoholic cirrhosis. We included 27 22 healthy individuals. A recently established flow cytometric approach used measure independent of sample count. Platelet further investigated using light transmission aggregometry (LTA) (for count >100 × 109/L). agonists were adenosine diphosphate, thrombin receptor-activating peptide,...
Abstract Introduction The rate of D‐alloimmunization amongst RhD‐negative recipients RhD‐positive red blood cell (RBC) transfusions is not certain. Recipients with a short duration between the index transfusion and last antibody detection test that did show anti‐D might become D‐alloimmunized in future. A regression model was developed to predict how often such patients develop future help account for immunohematological uncertainty accompanies having serological follow up periods. Methods...
Summary Deficiencies in many of the complement proteins and their regulatory molecules have been described a variety diseases, such as recurrent infections, systemic lupus erythematosus (SLE) renal may be linked to deficiency system. Screening for defects is therefore great importance. In this study, we present novel improved enzyme-linked immunosorbent assays functional assessment three individual pathways The method applicable at high serum concentrations demonstrate that it minimizes both...
Using potentially out-of-group blood components, like low titer A plasma and O whole blood, in the resuscitation of trauma patients is becoming increasingly popular. However, very little known whether donors' anti-A and/or anti-B titers change over time repeated measurements on same donor are required to ensure that each donation produces a product.The were measured 56 healthy adult volunteers (47 donors; nine center personnel) every 3 months for 12 consecutive using an automated solid phase...
SUMMARY Objectives Next‐generation sequencing (NGS) for the determination of rare blood group genotypes was tested in 72 individuals from different ethnicities. Background Traditional serological‐based antigen detection methods, as well genotyping based on specific single nucleotide polymorphisms (SNPs) or variants (SNVs), are limited to detecting only a number known antigens alleles. NGS methods do not have this limitation. Methods using Ion torrent Personal Genome Machine (PGM) performed...
Reduced platelet (PLT) function during storage has been shown for buffy-coat-derived concentrates (BCP) and apheresis units (AP), while whole blood (WB) it not well studied. The aim of this study was to investigate PLT in these products throughout using a novel flow cytometric assay.Flow measurement agonist-induced aggregation, CD62P expression PAC-1 binding BCP, AP (1-9 days at 20°C) WB (1-21 2-6°C).PLT-aggregation capacity decreased from day 1 7 almost all product-agonist combinations (P =...
BACKGROUND Transfusion of group O blood to non‐O recipients, or transfusion D– D+ can result in shortages blood, respectively. This study investigated RBC utilization patterns at hospitals around the world and explored context policies that guide ABO D type selection practices. STUDY DESIGN AND METHODS was a retrospective on data from 2013 calendar year. included survey component asked about hospital practices collection where participants submitted information unit disposition including...
BACKGROUND: Concern has been raised about the quality of white cell (WBC)‐reduced red cells (RBCs) obtained by bedside filtration. The performance and workload a routine filter have compared to laboratory two blood bank systems. STUDY DESIGN AND METHODS: Buffy coat‐depleted saline‐ adenine‐glucose‐mannitol (SAGM) RBCs (90 units) were prepared. Thirty units filtered with each systems, 30 (but not transfused) in clinical department after 8 24 days storage. lost postfiltration WBC content...
The novel coronavirus (SARS-Cov-2) that was first reported in Wuhan, China, and provokes the COVID-19 disease has developed into a pandemic with hundreds of thousands people infected. Many governments have enforced social isolation protocols on their citizens, which led to closure many large public gatherings order limit spread virus. These closures could reasonably be expected affect blood collections, thereby presaging shortages for transfusion. On other hand, steps such as postponement...
Abstract Background Reduced D antigen on red blood cells (RBCs) may be due to “partial” phenotypes associated with loss of epitope(s) and risk for alloimmunization or “weak” that do not lack major epitopes absence clinical complications. Genotyping samples weak discrepant typing is recommended guide transfusion RhIG prophylaxis. The goal was compare the impact RHD genotyping practice in two centers serving different populations. Study Design Methods Fifty‐seven from Denmark 353 United States...
Abstract Background There is mounting evidence that systemic uptake of food allergens key to triggering anaphylaxis. However, direct proof for this theory still lacking. The purpose study was quantify the absorption and determine kinetics immunoreactive peanut protein in relation allergic response human. Methods Quantitative assays including mass spectrometry, dot blots Western blotting were developed level Ara h 2 human serum. double monoclonal sandwich ELISA applied absorbed 6, basophil...
Abstract Background Continuous renal replacement therapy (CCRT) is a frequently used modality for the support of intensive care patients with acute kidney injury (AKI). Nevertheless, there are no objective criteria discontinuation CRRT. The purpose this study was to investigate whether urine neutrophil gelatinase-associated lipocalin (uNGAL) alone or in combination output could be as diagnostic test function recovery ICU on Methods This single-centre prospective observational including...
Abstract Background Thromboelastogram testing is increasingly being used to manage patients with massive bleeding. An earlier study found that the test results were influenced by hematocrit (Hct) and platelet (PLT) concentrations. This sought determine if these factors confounded of a different manufacturer's thromboelastography testing. Methods Using freshly collected whole blood from volunteers stored red cells (RBC) plasma, was manipulated achieve Hct values PLT Each reconstituted sample...
ABO-incompatible kidney transplantation is possible after pre-treatment with rituximab, intravenous immunoglobulin and basiliximab combined tacrolimus, mycophenolate mofetil prednisolone. We report on the first patient treated this protocol who developed acute antibody-mediated rejection (Banff grade II IgG deposits) caused by ABO antibodies (anti-B). Anti-rejection treatment anti-B-specific immunoadsorption, methylprednisolone efficiently cleared deposited from allograft re-established...
The persistence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is a matter importance regarding the disease 19 (COVID-19) pandemic. To observe antibody dynamics, 105 blood donors, positive for SARS-CoV-2 by lateral flow test within seroprevalence study, were included in this study. Thirty-nine (37%) donors confirmed total Ig Wantai enzyme-linked immunosorbent assay (ELISA). Three (8%) group 39 reported and 26/39 (67%) mild to moderate COVID-19 symptoms. By further...
Prophylactic anti-D is a very safe and effective therapy for the suppression of immunization thus prevention haemolytic disease foetus newborn. However, migration from countries with low health standards substantial cuts in public expenses have increased incidence many "developed" countries. Therefore, this forum focuses on prenatal monitoring treatment strategies pregnancies alloimmunization. The following questions were addressed, response was obtained 12 centres, mainly Europe.
To understand the worldwide scope of RBC crossmatching and issuing practices measure efficiency using a novel quality indicator, crossmatch/issue (C/I) ratio.An electronic survey was disseminated to hospital transfusion services collecting details about practices. Respondents were asked enumerate number RBCs crossmatched issued at their institutions during 2014 calendar year calculate C/I ratio.Fifty-two responses received, mostly from North American (28/52, 54%). The crossmatch most common...
Transfusion practice is reported to differ considerably between countries. Comparisons often rely on transfusion rates, incidence - or prevalence rates. In this paper, the one-year period rate (1-YPPR) of red cells (RBC) presented. data, demographic data and patient were retrospectively combined calculate sex diagnosis specific age standardized 1-YPPR s RBC for complete population in a Danish county. During calendar year 2006, 4427 patients received Funen County. The crude was 9.2/1000...