A5083887407- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic diversity and population structure
- Fish Biology and Ecology Studies
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Prenatal Screening and Diagnostics
- Genomics and Phylogenetic Studies
- Fish biology, ecology, and behavior
- Molecular Biology Techniques and Applications
- Evolution and Genetic Dynamics
- DNA Repair Mechanisms
- Animal Behavior and Reproduction
- Parasite Biology and Host Interactions
- Environmental DNA in Biodiversity Studies
- Plant Virus Research Studies
- Forensic and Genetic Research
- Subterranean biodiversity and taxonomy
- Gynecological conditions and treatments
- Craniofacial Disorders and Treatments
- Animal Ecology and Behavior Studies
- Neonatal skin health care
- Epigenetics and DNA Methylation
- Fish Ecology and Management Studies
Jena University Hospital
2016-2024
Friedrich Schiller University Jena
2016-2024
Universidade Federal de São Carlos
2017-2022
LIDSEN Publishing Inc. (United States)
2021
Institute of Human Genetics
2021
Abstract Most of snakes exhibit a ZZ/ZW sex chromosome system, with different stages degeneration. However, undifferentiated chromosomes and unique Y sex-linked markers, suggest that an XY system has also evolved in ancestral lineages. Comparative cytogenetic mappings revealed several genes share ancestry among X, Z chromosomes, implying ZW may have undergone transitions during serpent’s evolution. In this study, we performed comparative analysis to identify homologies across (Henophidia)...
Although fishes have traditionally been the subject of comparative evolutionary studies, few reports concentrated on application multipronged modern molecular cytogenetic techniques (such as genomic hybridization = CGH and whole chromosome painting WCP) to analyze deeper karyotype evolution specific groups, especially historically neglected small-sized ones. Representatives family Lebiasinidae (Characiformes) are a notable example, where only investigations conducted thus far. Here, we aim...
We present the first cytogenetic data for Lebiasinabimaculata and L.melanoguttata with aim of (1) investigating evolutionary events within Lebiasina their relationships other Lebiasinidae genera (2) checking between Ctenoluciidae. Both species have a diploid number 2n = 36 similar karyotypes microsatellite distribution patterns but contrasting C-positive heterochromatin CMA3+ banding patterns. The remarkable interstitial series occurring in is absent L.bimaculata. Accordingly, L.bimaculata...
Abstract Darevskia rock lizards is a unique complex taxa, including more than thirty species, seven of which are parthenogenetic. In mixed populations lizards, tri- and tetraploid forms can be found. The most important issues in the theory reticulate evolution origin parthenogenetic species their taxonomic position. However, there little data on how meiosis proceeds these species. present work reports results cytogenetics diploid – D. unisexualis . Here we detail meiotic prophase I...
The Neotropical armored catfish genus Harttia presents a wide variation of chromosomal rearrangements among its representatives. Studies indicate that translocation and Robertsonian have triggered the karyotype evolution in genus, including differentiation sex chromosome systems. However, few studies used powerful tools, such as comparative whole painting, to clarify this highly diversified scenario. Here, we isolated probes from X1 (a 5S rDNA carrier) X2 45S chromosomes punctata, which...
Hybrid parthenogenetic animals are an exceptionally interesting model for studying the mechanisms and evolution of sexual asexual reproduction. A diploid lizard Darevskia unisexualis is a result ancestral cross between maternal species raddei nairensis paternal valentini presents unique opportunity cytogenetic computational analysis hybrid karyotype. Our previous results demonstrated significant divergence pericentromeric DNA sequences parental species; however, in-depth comparative study...
Species with 'young' or nascent sex chromosomes provide unique opportunities to understand early evolutionary mechanisms (e.g. accumulation of repetitive sequences, cessation recombination and gene loss) that drive the evolution chromosomes. Among vertebrates, fishes exhibit highly diverse a wide spectrum sex-determining chromosomes, ranging from cryptic differentiated ones, as well as, simple multiple chromosome systems. Such variability in morphology composition not only exists within...
Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum Scleropages) distributed in South America, Asia, Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed set cytogenetic genomic approaches, including i) molecular analyses using C- CMA3/DAPI staining, repetitive DNA mapping, comparative hybridization (CGH), Zoo-FISH, along ii) genotypic single nucleotide polymorphisms (SNPs)...
The Neotropical genus Harttia comprises species with extensive chromosomal remodeling and distinct sex chromosome systems (SCSs). So far, three different SCSs male heterogamety have been characterized in the group. In some species, presence of XX/XY1Y2 SCS is associated a decrease diploid numbers several rearrangements, although direct relation to differentiation has not shown yet. Here, we aimed investigate processes that led establishment rare track its evolutionary history among other...
Oplegnathus fasciatus and O. punctatus (Teleostei: Centrarchiformes: Oplegnathidae), are commercially important rocky reef fishes, endemic to East Asia. Both species present an X1X2Y sex chromosome system. Here, we investigated the evolutionary forces behind origin differentiation of these chromosomes, with aim elucidate whether they had a single or convergent origin. To achieve this, conventional molecular cytogenetic protocols, involving mapping repetitive DNA markers, comparative genomic...
Cytogenetically visible chromosomal imbalances in humans are deleterious and adverse the majority of cases. However, healthy persons living with range several megabasepairs (Mbps) size, like carriers small Supernumerary Marker Chromosomes (sSMCs) exist.The identification sSMC euchromatic centromere-near (ECN) led to following proposal: ECN-regions do not contain any dosage sensitive genes. Due own previous work, dosage-insensitive pericentric were already determined an accuracy 0.3 5 Mbp....
Hepsetidae is a small fish family with only the genus Hepsetus, six described species distributed throughout South, Central and Western regions of Africa, showing close relationship Alestidae some Neotropical families. However, no cytogenetic information available for both species, thus preventing any evolutionary comparative studies at chromosomal level. In present study, we are providing new data Hepsetus odoe, including standard karyotype, C-banding, repetitive DNAs mapping, genomic...
Abstract Notopteridae (Teleostei, Osteoglossiformes) represents an old fish lineage with ten currently recognized species distributed in African and Southeastern Asian rivers. Their karyotype structures diploid numbers remained conserved over long evolutionary periods, since lineages diverged approximately 120 Mya. However, a significant genetic diversity was already identified for these using molecular data. Thus, why the relationships within are so diverse at genomic level but terms of...
Translocation between sex-chromosomes and autosomes generates multiple sex-chromosome systems. It happens unexpectedly, therefore, the evolutionary meaning is not clear. The current study shows a sex chromosome system comprising three different pairs in Taiwanese brown frog (Odorrana swinhoana). male-specific translocations created of six sex-chromosomes, ♂X1Y1X2Y2X3Y3-♀X1X1X2X2X3X3. unique that occurred among out members potential sex-determining chromosomes, which are known to be involved...
Solea senegalensis (Kaup, 1858) is a commercially important flatfish species, belonging to the Pleuronectiformes order. The taxonomy of this group has long been controversial, and karyotype order presents high degree variability in diploid number, derived from chromosomal rearrangements such as Robertsonian fusions. Previously it proposed that large metacentric chromosome S. arises kind rearrangement proto-sex chromosome. In work, origin tested by Zoo-FISH technique applied two species...
Although crocodilians have attracted enormous attention in other research fields, from the cytogenetic point of view, this group remains understudied. Here, we analyzed karyotypes eight species formally described Alligatoridae family using differential staining, fluorescence situ hybridization with rDNA and repetitive motifs as a probe, whole chromosome painting (WCP), comparative genome hybridization. All Caimaninae diploid number (2n) 42 dominated by acrocentric chromosomes, contrast to...
Giant lampbrush chromosomes (LBCs) typical for growing oocytes of various animal species are characterized by a specific chromomere-loop appearance and massive transcription. Chromomeres represent universal units chromatin packaging at LBC stage. While quite good progress has been made in investigation LBCs structure function, chromomere organization still remains poorly understood. To extend our knowledge on organization, we applied microdissection to chicken LBCs. In particular, 31 5...
Karyotypes of two cryptic species parasitoid Hymenoptera with n = 5 and 6 belonging to the Lariophagus distinguendus (Förster, 1841) complex, which includes cosmopolitan parasitoids coleopteran stored-product pests, were studied using glass-needle based microdissection, reverse cross-species fluorescence in situ hybridisation (FISH). This experiment strongly indicates that largest metacentric chromosome karyotype originated from a particular fusion between only acrocentric smaller set 6,...
We performed a comparative cytogenomic analysis of cultured and uncultured uterine leiomyoma (UL) samples. The experimental approach included karyotyping, aCGH, verification the detected chromosomal abnormalities by metaphase interphase FISH, MED12 mutation telomere measurement Q-FISH. An abnormal karyotype was in 12 out 32 UL In five karyotypically ULs, mutations were found. ULs present mostly complex rearrangements, including chromothripsis. both normal telomeres ~40% shorter than...