A5081131048- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hemophilia Treatment and Research
- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Blood properties and coagulation
- Immune Cell Function and Interaction
- Computational Drug Discovery Methods
- Pneumocystis jirovecii pneumonia detection and treatment
- Eosinophilic Esophagitis
- Vibrio bacteria research studies
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Tuberculosis Research and Epidemiology
- Helicobacter pylori-related gastroenterology studies
- Chemokine receptors and signaling
- Receptor Mechanisms and Signaling
- Hepatitis B Virus Studies
- Neuropeptides and Animal Physiology
- Antibiotic Resistance in Bacteria
- Erythrocyte Function and Pathophysiology
- HIV/AIDS Impact and Responses
- Cancer-related gene regulation
- Veterinary medicine and infectious diseases
Hôpital La Rabta
2013-2016
Tunis El Manar University
2007-2011
Tunis University
2007-2011
Hôpital Charles-Nicolle
2003-2008
Summary. Combined deficiency of coagulation factor V (FV) and VIII (FVIII) (F5F8D) is a rare autosomal recessive disorder characterized by mild‐to‐moderate bleeding reduction in FV FVIII levels plasma. F5F8D caused mutations one two different genes, LMAN1 MCFD2 , which encode proteins that form complex involved the transport from endoplasmic reticulum to Golgi apparatus. Here, we report identification novel mutation Asp89Asn gene Tunisian patient. In encoded protein, this causes substitution...
Hemophilia A is an X linked recessive hemorrhagic disorder caused by mutations in the F8 gene that lead to qualitative and/or quantitative deficiencies of coagulation factor VIII (FVIII). Molecular diagnosis hemophilia challenging because high number different causative are distributed throughout large gene. studies these essential order reinforce our understanding their pathogenic effect responsible for disorder. In this study we have performed molecular analysis 28 Tunisian patients and...
In this study we report the prevalence of antiretroviral drug resistant HIV-1 genotypes virus isolated from Djiboutian patients who failed first-line therapy (ART) and ART naïve patients. A total 35 blood samples 16 showed failure (>1000 viral genome copies/ml) 19 ART-naïve were collected in Djibouti October 2009 to December 2009. Both protease (PR) reverse transcriptase (RT) genes amplified sequenced using National Agency for AIDS Research (ANRS) protocols. The Stanford HIV database...
We compared the response of two standard 3-drug regimens containing two-nucleoside reverse transcriptase inhibitor (Zidovudine +Lamivudine) plus either a protease (PIs) (Indinavir) or nonnucleoside (NNRTIs) (Efavirenz) among treatment-naïve treatmentexperienced HIV-infected persons. The obtained results will be to clinical trial findings. Through retrospective study, we virological and immunological 119 Tunisian HIV-1 infected patients (North Africa) who started for first time/ in salvage...
Combined factor V (FV) and VIII (FVIII) deficiency (F5F8D) is a rare autosomal recessive disorder caused by mutations in LMAN1 or MCFD2 genes which encode proteins that form complex involved the transport of FV FVIII from endoplasmic reticulum to Golgi apparatus. We report two novel gene one recurrent mutation combined unrelated Tunisian Muslim families. For first family patients were homozygous for new missense Asp81His exon 3 heterozygous second Val100Asp same exon. Replacement...
The development of inhibitors against factor 8 (F8) is the most serious complication replacement therapy with F8 in children severe hemophilia. It was suggested that mismatched may be a risk for anti-factor alloantibodies. Recently four single nucleotide polymorphisms (SNPs) encoding six distinct haplotypes, designated H1 through H6, were studied different populations. Two SNPs are components A2 and C2 immunodominant-inhibitor epitopes.The aim this study to determine types haplotypes...
The chemokine receptor 5 (CCR5) belongs to the superfamily of serpentine G protein-coupled receptors (GPCRs). DRY motif (Asp, Arg, Tyr) intracellular loop 2 (ICL2), which is highly conserved in GPCRs has been shown be essential for stability folding CCR5 and interaction with β-arrestin. But molecular mechanism by it recognizes interacts β-arrestin not elucidated. In present study, we described active state structure using normal mode analysis characterized binding cleft CCR5-ICL2 SABRE©...
Inherited factor VII (FVII) deficiency is a rare disorder characterized by bleeding phenotype varying from mild to severe. To date, more than 200 mutations have been described along the F7 gene encoding for FVII. The aim of this study was identification genetic defects underlying FVII in 10 patients belonging eight unrelated families North provinces Tunisia. Mutation detection performed sequencing whole coding region, exon-intron boundaries and about 400 bp promoter region. We identified 5...
AbstractWe report the identification of a novel CC chemokine receptor 5 (CCR5) variant that seems associated with resistance to HIV-1 infection. The V130I mutation CCR5 is located in intracellular loop ICL2 known as DRY box and described literature nonsynonymous present nonhuman primates group. Extensive molecular modeling dynamics simulations were performed elucidate mechanism by which may induce conformational change folding protein prevent interaction β-arrestin protein. Our study...
Purpose of the Study: This study was conducted to detect and characterize genes encoding extended spectrum β-lactamases associated (carbapenemases Ambler Class C β-lactamases).Patients Methods: In 2011, out 65 non-duplicative Klebsiellapneumoniae collected from blood culture at Charles Nicolle hospital Tunisia, 36 were resistant 3rd generation cephalosporin.Results: All strains showed a double disk synergy test positive. They mainly isolated in intensive care unit (31%). frequently most...