A5062642412- RNA Interference and Gene Delivery
- Bacterial Identification and Susceptibility Testing
- Bone Tissue Engineering Materials
- Alkaline Phosphatase Research Studies
- Radiopharmaceutical Chemistry and Applications
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- biodegradable polymer synthesis and properties
- Tissue Engineering and Regenerative Medicine
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Antimicrobial Resistance in Staphylococcus
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Fluorine in Organic Chemistry
- Connective tissue disorders research
- Extracellular vesicles in disease
- Bacterial biofilms and quorum sensing
Leipzig University
2020-2023
Brandenburg University of Technology Cottbus-Senftenberg
2018
Pathogens frequently associated with multi-drug resistant (MDR) phenotypes, including extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and Acinetobacter baumannii isolated from horses admitted to horse clinics, pose a risk for animal patients personnel in clinics. To estimate current rates of colonization, total 341 equine were screened carriage zoonotic indicator pathogens at hospital admission. Horses showing clinical signs colic (n = 233) or open wounds 108)...
RNA interference opened new approaches for disease treatment but safe and efficient cell delivery remains a bottleneck. Extracellular vesicles (EVs) are known to naturally shuttle RNA. Due their potent internalization low-cost scalability, milk-derived EVs in particular considered promising systems. However, low drug loading currently impedes use. Here, innovative exogenous strategies small interfering (siRNA) explored systematically compared regarding encapsulation efficiency, capacity,...
Continuing introduction of multi-drug resistant, zoonotic pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) in horse clinics challenges the biosafety employees and animal patients. This study was aimed to determine occurrence mobile genetic elements facilitating survival early stages invasive infection different host species, including humans horses, MRSA carried by equine patients admitted a large clinic. A total 341 were investigated for carriage hygiene screening...
Dickkopf-1 (DKK1), a Wnt inhibitor secreted by bone marrow stromal cells (MSC), is known to play an important role in long-term non-union fracture defects and glucocorticoid induced osteoporosis. Mitigating its effects early could improve osteogenesis defect healing. Here, we applied biomaterial strategy deplete environment from DKK1 scavenging the protein via macromer-based covalently decorated with sulfated hyaluronan (sHA3). The material consisted of cross-copolymerized three-armed...
Convection-enhanced delivery (CED) has been introduced as a concept in cancer treatment to generate high local concentrations of anticancer therapeutics and overcome the limited diffusional distribution, e.g., brain. RNA interference provides interesting therapeutic options fight cells but requires nanoparticulate (NP) carriers with size below 100 nm well low zeta potential for CED application. In this study, we investigated calcium phosphate NPs (CaP-NPs) siRNA Since CaP-NPs tend aggregate,...
The local release of complexed siRNA from biomaterials opens precisely targeted therapeutic options. In this study, was loaded to gelatin microparticles cross-linked (cGM) with an anhydride-containing oligomer (oPNMA). We aggregated these siRNA-loaded cGM human mesenchymal stem cells (hMSC) microtissues and stimulated them osteogenic supplements. An efficient knockdown chordin, a BMP-2 antagonist, caused remarkably increased alkaline phosphatase (ALP) activity in the microtissues. cGM, as...
This study describes the synthesis, radiofluorination and purification of an anionic amphiphilic teroligomer developed as a stabilizer for siRNA-loaded calcium phosphate nanoparticles (CaP-NPs). As stabilizing amphiphile accumulates on nanoparticle surfaces, fluorine-18-labeled polymer should enable to track distribution CaP-NPs in brain tumors by positron emission tomography after application convection-enhanced delivery. At first, unmodified was synthesized with number average molecular...
High serum levels of Wnt antagonists are known to be involved in delayed bone defect healing. Pharmaceutically active implant materials that can modulate the micromilieu defects with regard therefore considered promising support regeneration. In this study, we show versatility a macromer based biomaterial platform systematically optimize covalent surface decoration high-sulfated glycosaminoglycans (sHA3) for efficient scavenging antagonist sclerostin. Film surfaces representing scaffold...