A5000294718- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Polymer Surface Interaction Studies
- DNA and Nucleic Acid Chemistry
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Bone Tissue Engineering Materials
- Antimicrobial Peptides and Activities
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- Inhalation and Respiratory Drug Delivery
- Graphene and Nanomaterials Applications
- Dendrimers and Hyperbranched Polymers
- Surfactants and Colloidal Systems
- Extracellular vesicles in disease
- Neonatal Respiratory Health Research
- Supramolecular Self-Assembly in Materials
- Advanced Polymer Synthesis and Characterization
- Photodynamic Therapy Research Studies
- Silk-based biomaterials and applications
- Spectroscopy and Quantum Chemical Studies
- Streptococcal Infections and Treatments
- Chemical Synthesis and Analysis
Leipzig University
2019-2025
Leipzig University of Applied Sciences
2022
Martin Luther University Halle-Wittenberg
2011-2021
Biochemical Society
2019
Luther University
2017-2019
Philipps University of Marburg
2015
Wittenberg University
2013
University of Stuttgart
1976
RNA interference opened new approaches for disease treatment but safe and efficient cell delivery remains a bottleneck. Extracellular vesicles (EVs) are known to naturally shuttle RNA. Due their potent internalization low-cost scalability, milk-derived EVs in particular considered promising systems. However, low drug loading currently impedes use. Here, innovative exogenous strategies small interfering (siRNA) explored systematically compared regarding encapsulation efficiency, capacity,...
Abstract The energies of the free exciton and four excitons bound to neutral acceptors (A°, X) are determined by either reflection, absorption, or emission with high purity p‐type GaSb. data give evidence that may be described alternatively as an electron a donor‐like A + ‐centre. For one particular deep ‐centre, transitions from donor states ‐centre observed besides decay. p‐GaSb is especially favourable for observing ‐centres, because position Fermilevel. Conditions under which they...
The immediate release of chemotherapeutics at the target site, along with no premature in circulation is always challenging. purpose this study was to develop a stimuli responsive drug delivery system, composed lipid supported mesoporous silica nanoparticles (MSNPs) for triggered site and simultaneously avoiding release. MSNPs higher loading capacity very slow were designed so as enhance by FDA approved US-irradiation. Doxorubicin, model drug, perfluoropentane (PFP) US material, entrapped...
The envelope (E) protein of SARS-CoV-2 participates in virion encapsulation and budding at the membrane endoplasmic reticulum Golgi intermediate compartment (ERGIC). positively curved topology required to fit an 80 nm viral particle is energetically unfavorable; therefore, proteins must facilitate ERGIC curvature alteration. To study possible role E this mechanism, we examined structural modification host lipid by using synchrotron-based X-ray methods. Our reflectometry results on...
Biomaterials, which release active compounds after implantation, are an essential tool for targeted regenerative medicine. In this study, thin multilayer films loaded with lipid/DNA complexes (lipoplexes) were designed as surface coatings in situ transfection applicable tissue engineering and The film production embedding of lipoplexes based on the layer-by-layer (LbL) deposition technique. Hyaluronic acid (HA) chitosan (CHI) used polyelectrolyte components. embedded plasmid DNA was...
Natural photosensitizers, such as curcumin or parietin, play a vital role in photodynamic therapy (PDT), causing light-mediated reaction that kills cancer cells. PDT is promising treatment option for glioblastoma, especially when combined with nanoscale drug delivery systems. The curcumin- parietin-loaded lipid nanoparticles were prepared via dual asymmetric centrifugation and subsequently characterized through physicochemical analyses including dynamic light scattering, laser Doppler...
Abstract A series of novel malonic acid diamides (second generation) with two long hydrophobic alkyl chains and an alkaline polar head group was synthesised characterised as a new class amino‐functionalised lipids. These peptide‐mimic lipids are suitable for polynucleotide transfer. The bear backbone consisting lysine unit unit. Six different head‐group structures, which vary in size number amino groups that can be protonated, were attached to the structure. Furthermore, used build...
In continuation of previous work, we present a new promising DNA carrier, OO4, highly effective peptide-mimicking lysine-based cationic lipid. The structural characteristics the polynucleotide carrier system OO4 mixed with commonly used co-lipid DOPE and saturated phospholipid DPPE have been studied in two-dimensional three-dimensional model systems to understand their influence on physical–chemical properties. phase behavior pure its mixtures was at air–water interface using Langmuir film...
Biomimetic surface coatings can be combined with conventional implants to mimic the extracellular matrix (ECM) of surrounding tissue make them more biocompatible. Layer-by-layer technique (LbL) used for making by alternating adsorption polyanions and polycations from aqueous solutions without need chemical reactions. Here, polyelectrolyte multilayer (PEM) systems is made hyaluronic acid (HA) as polyanion Collagen I (Col) polycation ECM connective tissue. The PEM are dexamethasone...
Ionizable lipids are a class of pharmaceutical excipients with main application in lipid nanoparticles for nucleic acid delivery. New ionizable needed to tune characteristics lipid-based delivery systems, e.g. stability, loading capacity and binding strength, as well bio-distribution. Herein, we present the synthesis three novel putative systems. Langmuir monolayer experiments classical surface pressure/area isotherm evaluation were used understand self-assembly behavior lipids. Additional...
Abstract Cationic liposome/DNA complexes can be used as nonviral vectors for direct delivery of DNA‐based biopharmaceuticals to damaged cells and tissues. To obtain more effective safer liposome‐based gene transfection systems, two cationic lipids with identical head groups but different chain structures are investigated respect their in vitro gene‐transfer activity, cell‐damaging characteristics, physicochemical properties. The activities the very different. Differential scanning...
The aggregation behavior of various zwitterionic helper phospholipids, such as DOPE, DOPC, and DPPC, in combination with two new cationic lipids, namely TH4 OH4 (second generation malonic acid diamides) different molar ratios was studied regard to their physical–chemical properties. Additionally, lipoplexes prepared from these lipid mixtures were characterized respect the transfection efficacy using an EGFP‐assay. fluid DOPE have shown comparable efficiency Lipofectamine 2000®. Furthermore,...
Four different binary lipid mixtures composed of a cationic and the zwitterionic colipids DOPE or DPPC, which show DNA transfer activities in cell culture models, were investigated at soft air/water interface to identify transfection efficiency determining characteristics. Langmuir films are useful models investigate interaction between two-dimensional model system by using surface sensitive techniques, namely, epifluorescence microscopy infrared reflection-absorption spectroscopy....
As a continuation of previous work, the self-assembly process cationic lipid formulations in presence and absence DNA was investigated with respect to binary mixtures suitable as polynucleotide carrier systems. The blends consist one malonic-acid-based varying alkyl chain pattern, either N-{6-amino-1-[N-(9Z)-octadec-9-enylamino]-1-oxohexan-(2S)-2-yl}-N'-{2-[N,N-bis(2-aminoethyl)amino]ethyl}-2-hexadecylpropandiamide () or...
Abstract A gene‐activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin‐film based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers the extracellular matrix, which are fabricated by layer‐by‐layer assembly. For further functionalization, DNA/lipid‐nanoparticles (lipoplexes) incorporated into multilayers. multilayer fabrication lipoplex deposition analyzed sensitive analytical...