A5076400091- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cancer Cells and Metastasis
- ATP Synthase and ATPases Research
- Histone Deacetylase Inhibitors Research
- Mitochondrial Function and Pathology
- CAR-T cell therapy research
- Cancer, Stress, Anesthesia, and Immune Response
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Calcium signaling and nucleotide metabolism
- Chemokine receptors and signaling
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Multicomponent Synthesis of Heterocycles
Universidad Nacional Autónoma de México
2009-2025
National Institute of Allergy and Infectious Diseases
2025
National Institutes of Health
2025
Instituto Nacional de Enfermedades Respiratorias
2011-2021
National Commission for the Knowledge and Use of Biodiversity
2011
Monash University
1997-2000
Box Hill Hospital
1997
ABSTRACT Wild-type Bacillus subtilis ferments 20 g/liter glucose in 48 h, producing lactate and butanediol, but not ethanol or acetate. To construct an ethanologenic B. strain, homologous recombination was used to disrupt the native dehydrogenase (LDH) gene ( ldh ) by chromosomal insertion of Zymomonas mobilis pyruvate decarboxylase pdc alcohol II adhB under control promoter. The values intracellular PDC ADHII enzymatic activities engineered BS35 strain were similar those found Escherichia...
BACKGROUNG: Biodegradable plastics can be made from polylactate, which is a polymer lactic acid. This compound produced renewable resources as substrates using microorganisms. Bacillus subtilis Gram-positive bacterium recognized GRAS microorganism (generally regarded safe) by the FDA. B. produces and secretes different kind of enzymes, such proteases, cellulases, xylanases amylases to utilize carbon sources more complex than monosaccharides present in environment. Thus, could potentially...
Inositol polyphosphate 5-phosphatases (5-phosphatase) hydrolyze the 5-position phosphate from inositol ring of phosphatidylinositol-derived signaling molecules; however, mechanism catalysis is only partially characterized. These enzymes play critical roles in regulating cell growth, apoptosis, intracellular calcium oscillations, and post-synaptic vesicular trafficking. The UCLA fold recognition server (threader) predicted that conserved 300-amino acid catalytic domain, common to all...
Solid tumors frequently present a heterogeneous tumor microenvironment. Because have the potential to proliferate quickly, consequence is reduction in nutrients, pH (<6.8), and hypoxic environment. Although it often assumed that clones show similar growth rate with little variations nutrient consumption, study shows how growth-specific (µ), specific rates of glucose, lactate, glutamine consumption (qS), lactate glutamate production (qP) 2D-cultured lung cells are affected by changes their We...
Metformin, caffeine, and dichloroacetate (DCA) have shown antitumor effects. The hypoxic tumor microenvironment can modulate drug response. We aimed to analyze the interaction of metformin with caffeine or DCA in lung cancer cells (HCC827) under normoxia hypoxia conditions. Cell viability was evaluated using crystal violet assay after individual combined treatment (21% O2) (1% Combination effects were analyzed isobolographic analysis. results show that conditions, combination (γ = 0.98 ±...
The 43 kDa inositol polyphosphate 5-phosphatase (5-phosphatase) hydrolyzes and thereby inactivates the second messenger molecules 1,4,5-trisphosphate [Ins(1,4,5)P3] 1,3,4,5-tetrakisphosphate in a signal terminating reaction. Recent studies have shown that platelet protein pleckstrin forms complex with activates Ins(1,4,5)P3 hydrolysis 2-fold [Auethavekiat, V., Abrams, C. S., & Majerus, P. W. (1997) J. Biol. Chem. 272, 1786−1790]. We now show another protein, 14-3-3ζ, of Ins(1,4,5)P3. Both...
Lung cancer is the leading cause of death worldwide and non-small cell lung carcinoma (NSCLC) most common type carcinomas. In adenocarcinomas, frequent histologic NSCLC, dendritic cells (DCs) are localized in close contact with tumor cells, tumor-infiltrating lymphocytes (TILs) observed peritumoral zones. no studies investigating density intratumoral DCs their impact on TILs have been performed. addition, role alarmin high-mobility group box1 (HMGB1) recruitment has not analyzed. present...
Lactic acidosis, glucose deprivation and hypoxia are conditions frequently found in solid tumors because, among other reasons, switch to Warburg effect secrete high levels of lactate, which decreases the pH (<6. 9) microenvironment. We hypothesized that lung cancer cells consume lactate induce mitochondrial biogenesis support survival proliferation lactic acidosis with even under hypoxia. examined adenocarcinoma cell lines (A-427 A-549), a breast line (MCF-7) non-transformed fibroblasts...
Lung cancer is the leading cause of death worldwide. Adenocarcinoma, most commonly diagnosed histologic type lung cancer, associated with smoking. Cigarette smoke promotes inflammation on airways, which might be mediated by Th17 cells. This inflammatory environment may contribute to tumor development. In contrast, some reports indicate that tumors induce immunosuppressive Treg cells dampen immune reactivity, supporting growth and progression. Thus, we aimed analyze whether chronic or...
Lactic acidosis (3 to 40 mM, pH < 6.9) is a condition found in solid tumors because tumor cells have high rate of glucose consumption and lactate production even the presence oxygen; nevertheless, microenvironment might still provide sufficient supply. has been proposed shift metabolism from aerobic glycolysis towards oxidative phosphorylation (OXPHOS). We tested if lung cultured under lactic their OXPHOS by consuming extracellular lactate, increasing growth rate. analyzed adenocarcinoma...
The ribonucleoproteins (hnRNPs) have important roles in multiple aspects of nucleic acid metabolism and the regulation different cellular processes. Abnormal expression hnRNPs has been reported several types cancer including lung, pancreatic, gastric carcinomas. Heterogenous tumor cell populations generate a microenvironment that can present normoxic, hypoxic, or acidic regions. analysis hnRNP transcriptional responses considering changing nature is to understand survival under stress...
Abstract Adenosine‐5‐triphosphate (ATP) plays a fundamental role in many cellular processes such as transport, central carbon metabolism, biosynthetic reactions, macromolecular synthesis, signal transduction and division. In addition, the intracellular [ATP]/[ADP] ratio Escherichia coli an important controlling specific rates of growth (µ), glucose consumption (q Glc ) oxygen uptake O2 ), well transcriptome pattern cell, was recently reported. current study, energetic level (expressed ratio)...