A5071662847- Computational Drug Discovery Methods
- Essential Oils and Antimicrobial Activity
- Metabolomics and Mass Spectrometry Studies
- Traditional Chinese Medicine Analysis
- COVID-19 and Mental Health
- Tannin, Tannase and Anticancer Activities
- Phytoestrogen effects and research
- Phytochemicals and Antioxidant Activities
- Analytical Chemistry and Chromatography
- Long-Term Effects of COVID-19
- Healthcare Systems and Practices
- Chemical synthesis and alkaloids
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Health, Medicine and Society
- Natural Antidiabetic Agents Studies
- Ginseng Biological Effects and Applications
- Advanced Chemical Sensor Technologies
- Isotope Analysis in Ecology
- Heavy Metals in Plants
- Protein Interaction Studies and Fluorescence Analysis
- Diverse Scientific Research Studies
- Analytical Methods in Pharmaceuticals
- COVID-19 Pandemic Impacts
- Microbial Metabolism and Applications
University of Geneva
2015-2022
University of North Carolina at Greensboro
2020-2022
University of Lausanne
2015-2020
HES-SO University of Applied Sciences and Arts Western Switzerland
2020
University of Southampton
2020
Despite the value of mass spectrometry in modern natural products discovery workflows, it remains very difficult to compare data sets between laboratories. In this study we compared for same sample set from two different laboratories (quadrupole time-of-flight and quadrupole-Orbitrap) evaluated similarity these terms both features their ability describe chemical composition set. Somewhat surprisingly, sets, collected with appropriate controls replication, had low feature overlap (25.7%...
At the early drug discovery stage, high-throughput parallel artificial membrane permeability assay is one of most frequently used <i>in vitro</i> models to predict transcellular passive absorption. While thousands new chemical entities have been screened with assay, in general, permeation properties natural products scarcely evaluated. In this study, through a hexadecane was intestinal absorption representative set occurring products. Since are usually ingested for medicinal use as...
Wild or semi-wild edible greens (chórta) are an integral part of the traditional Greek Mediterranean diet due to their nutritional value, containing various phytonutrients beneficial human health. Water-based decoctions chórta widely consumed in alternative medicine as health promoting agents. This study examined chemical profile eight plants, Cichorium intybus, C. endivia, spinosum, Crepis sancta, Sonchus asper, Carthamus lanatus, Centaurea raphanina, and Amaranthus blitum, by UPLC-ESI-HRMS...
In Traditional Chinese Medicine (TCM), herbal preparations often consist of a mixture herbs. Their quality control is challenging because every single herb contains hundreds components (secondary metabolites). A typical 10 TCM formula was selected to develop an innovative strategy for its comprehensive chemical characterization and study the specific contribution each in exploratory manner. Metabolite profiling extract were acquired with liquid chromatography coupled high-resolution mass...
Abstract Introduction Non-pharmaceutical interventions (NPIs), such as handwashing, social distancing and face mask wearing, have been widely promoted to reduce the spread of COVID-19. This study aimed explore relationship between self-reported use NPIs COVID-19 infection. Methods We conducted an online questionnaire recruiting members UK public from November 2020 May 2021. The association illness reported was explored using logistic regression controlling for participant characteristics,...
Untargeted mass spectrometry (MS) metabolomics is an increasingly popular approach for characterizing complex mixtures. Recent studies have highlighted the impact of data preprocessing determining quality analysis. The first step in processing with untargeted requires that signal thresholds be selected which features (detected ions) are included dataset. Analysts face challenge knowing where to set these thresholds; setting them too high could mean missing relevant features, but low result a...
Ethnopharmacological data and ancient texts support the use of black hellebore (Helleborus odorus subsp. cyclophyllus, Ranunculaceae) for management treatment epilepsy in Greece.A pharmacological investigation root methanolic extract (RME) was conducted using zebrafish model to isolate identify compounds responsible a potential antiseizure activity provide evidence its historical use. In addition, comprehensive metabolite profiling this studied species proposed.The roots were extracted by...
We aimed to investigate use of infection control behaviours, preventative and therapeutic interventions, outcomes among respondents an online survey during the COVID-19 pandemic in China.
Herbal preparations (HPs) used in folk medicine are complex mixtures of natural products (NPs). Their efficacy vivo after ingestion depends on the uptake active ingredient, and, some cases, their metabolites, gastrointestinal tract. Thus, correlating bioactivities measured vitro and is a challenge. An extract Pueraria lobata rich different types isoflavones was to evaluate capacity viable porcine small intestine ex elucidate absorption HP constituents, metabolites. The identification...
Abstract Plants have a long history of use for their medicinal properties. The complexity botanical extracts presents unique challenges and necessitates the application innovative approaches to correctly identify quantify bioactive compounds. For this study, we used untargeted metabolomics explore antimicrobial activity Rumex crispus (yellow dock), member Polygonaceae family as an herbal remedy bacterial infections. Ultra-performance liquid chromatography coupled with high resolution...
Natural products are generally ingested as part of traditional herbal decoctions or in the current diet. However, natural product research, bioavailability secondary metabolites is often poorly investigated. In this work, a systematic study was carried out order to highlight physicochemical parameters that mainly influence passive intestinal absorption products. For this, representative set including alkaloids, coumarins, flavonoid aglycones and glycosides, carboxylic acids selected their...
Abstract Natural remedies are used as stand-alone treatments or additives to modern medicine control hyperglycemia. Here, we investigate the antidiabetic potential of Palauan traditional leaf decoction Phaleria nisidai (PNe) using a reverse pharmacology approach. In diet induced obese mice, dietary supplementation with PNe improves insulin sensitivity and promotes glucose uptake into adipose depots. vitro, triggers insulin-independent disposal murine human adipocytes by upregulating Glut1...
Background This study aimed to explore individual prevalence of respiratory symptoms and describe the Korean population's treatment approaches, preventive health behaviors, mental conditions during pandemic. Methods We analyzed responses from an online nationwide survey, conducted between February 2021 May 2021, about people's experiences Statistical analysis was also performed see if there were any significant differences in prevention strategies different groups respondents (between those...
At early drug discovery stage, the high-throughput Parallel Artificial Membrane Permeability Assay (PAMPA) is one of most frequently used in vitro model to predict transcellular passive absorption. While thousands new chemical entities have been screened with PAMPA, general permeation properties natural products (NPs) scarcely evaluated. In this study, Hexadecane (HDM) PAMPA was intestinal absorption a set NPs. Alkaloids, coumarins and methoxylated flavonoid aglycones showed favourable...
SummaryNatural remedies are used as stand-alone treatments or additives to modern medicine control hyperglycemia. Here, we investigate the antidiabetic potential of Palauan traditional leaf decoction Phaleria nisidai (PNe) using a reverse pharmacology approach. In diet induced obese mice, dietary supplementation with PNe improves insulin sensitivity and promotes glucose uptake into adipose depots. vitro, triggers insulin-independent disposal murine human adipocytes by upregulating Glut1...
The increase in the prevalence of overweight/obese people worldwide has reached a qualified epidemic stage with more than one billion overweight and at least 400 million clinically obese patients.[1] In search for new treatments to improve management diseases related obesity, Asian herbal medicines provide prospects high therapeutic potential, but empirical knowledge be scientifically validated[2]. Using novel hypothesis driven screening approach [3], root Pueraria montana var. lobata (PL)...
Atopic dermatitis (atopic eczema) is a common inflammatory skin disorder, treated in Western medicine by topical corticosteroids and emollients [1]. Recently, an open label clinical study showed the efficacy of new formula based on mixture ten herbs used Traditional Chinese Medicine (TCM) [2]. The quality control for such multi-herb challenging. aim this work was to design innovative strategy comprehensive rational standardisation. It depth high resolution metabolite profiling TCM formula,...
Herbal extracts used in folk medicine are complex mixtures of natural products (NPs). Their efficacy vivo after ingestion depends on their uptake (and sometimes metabolites) the gastrointestinal tract. The correlation bioactivities measured vitro with is a challenge since bioavailability and biotransformation can have an impact.[1] We validated ex model using viable porcine small intestine to elucidate effects absorption intestinal metabolism drug (manuscript preparation). Porcine closely...