A5080835594- Plant Molecular Biology Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Virus-based gene therapy research
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Plant biochemistry and biosynthesis
- Polyoxometalates: Synthesis and Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Bacteriophages and microbial interactions
- Plant Stress Responses and Tolerance
- Light effects on plants
- Lipid metabolism and biosynthesis
- Amino Acid Enzymes and Metabolism
- Crystallography and molecular interactions
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Cancer Research and Treatments
- Viral Infections and Immunology Research
Université Grenoble Alpes
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Centre National de la Recherche Scientifique
2015-2024
Laboratoire de Physiologie Cellulaire
2014-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
Laboratoire Physiologie Cellulaire & Végétale
2015-2024
CEA Grenoble
2015-2024
Centre de Biologie Structurale
2024
Biotechnology Institute
2018
Institut de Recherches en Technologies et Sciences pour le Vivant
2014-2017
Soft chemical methods were used for preparing the new class of composite materials presented here. One example is [{Cu(4,4′-bpy)}4Mo8O26] (4,4′-bpy = 4,4′-bipyridine), whose unique structure (shown on right) consists [Mo8O26]4− clusters embedded in a web {Cu(4,4′-bpy)} one-dimensional chains.
Salicylic acid (SA) is a phytohormone best known for its role in plant defense. It synthesized response to diverse pathogens and responsible the large scale transcriptional induction of defense-related genes establishment systemic acquired resistance. Surprisingly, given importance defense, an understanding underlying enzymology lacking. In Arabidopsis thaliana, pathogen-induced accumulation SA requires isochorismate synthase (AtICS1). Here, we show that AtICS1 plastid-localized, stromal...
Caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase (COMT) from alfalfa is an S-adenosyl-l-Met–dependent O-methyltransferase involved in lignin biosynthesis. COMT methylates caffeoyl- and 5-hydroxyferuloyl–containing acids, aldehydes, alcohols vitro while displaying a kinetic preference for the aldehydes over free acids. The 2.2-Å crystal structure of complex with S-adenosyl-l-homocysteine (SAH) ferulic (ferulate form), as well 2.4-Å SAH 5-hydroxyconiferaldehyde, provide structural...
Some basil varieties are able to convert the phenylpropenes chavicol and eugenol methylchavicol methyleugenol, respectively. Chavicol O-methyltransferase (CVOMT) (EOMT) cDNAs were isolated from sweet variety EMX-1 using a biochemical genomics approach. These encode proteins that 90% identical each other very similar several isoflavone O-methyltransferases such as IOMT, which catalyzes 4'-O-methylation of 2,7,4'-trihydroxyisoflavanone. On hand, CVOMT1 EOMT1 related only distantly (iso)eugenol...
Recently, a novel family of methyltransferases was identified in plants. Some members this newly discovered and recently characterized methyltransferase catalyze the formation small-molecule methyl esters using S-adenosyl-l-Met (SAM) as donor carboxylic acid–bearing substrates acceptors. These enzymes include SAMT (SAM:salicylic acid carboxyl methyltransferase), BAMT (SAM:benzoic JMT (SAM:jasmonic methyltransferase). Moreover, other plant have been found to N-methylation caffeine precursors....
The use of a novel mixed-valence pentavanadate phosphate cluster as building block has made possible the low-temperature hydrothermal self-assembly new three-dimensional square pyramidal−tetrahedral framework vanadium phosphates [HN(CH2CH2)3NH]K1.35[V5O9(PO4)2]·xH2O (1) and Cs3[V5O9(PO4)2]·xH2O (2), from structurally simple starting materials. These materials possess some lowest densities largest cavities thus far observed in open-framework solid-state degree curvature, size, shape, charge...
Acyl acid amido synthetases of the GH3 family act as critical prereceptor modulators plant hormone action; however, molecular basis for their selectivity is unclear. Here, we report crystal structures benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1. These structures, combined with biochemical analysis, define features conjugation amino acids to diverse acyl substrates highlight importance conformational changes in carboxyl-terminal domain...
It is generally recognised that novel antiviral drugs, less prone to resistance, would be a desirable alternative current drug options in order able treat potentially serious influenza infections. The viral polymerase, which performs transcription and replication of the RNA genome, an attractive target for drugs since potent polymerase inhibitors could directly stop at early stage. Recent structural studies on functional domains heterotrimeric comprises subunits PA, PB1 PB2, open way...
Abstract Chlorogenic acids (CGAs) are a group of phenolic secondary metabolites produced by certain plant species and an important component coffee (Coffea spp.). The CGAs have been implicated in biotic abiotic stress responses, while the related shikimate esters key intermediates for lignin biosynthesis. Here, two hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyl transferases (HCT/HQT) from were biochemically characterized. We show, to our knowledge first time, that vitro, HCT...
Significance Plants require precise control over growth regulators during development and in their responses to biotic abiotic stresses. One strategy for modulating levels of bioactive phytohormones is conjugate these molecules amino acids using acyl acid amido synthetases the Gretchen Hagen 3 (GH3) protein family. Typically, GH3 proteins modify one type phytohormone. Structural studies, along with vitro planta biochemical analyses, reveal that GH3.5 from model plant Arabidopsis thaliana...
The Evening Complex (EC), composed of the DNA binding protein LUX ARRHYTHMO (LUX) and two additional proteins EARLY FLOWERING 3 (ELF3) ELF4, is a transcriptional repressor complex core component plant circadian clock. In addition to maintaining oscillations in clock gene expression, EC also participates temperature light entrainment, acting as an important environmental sensor conveying this information growth developmental pathways. However, molecular basis for specificity...
Abstract In plants, MADS domain transcription factors act as central regulators of diverse developmental pathways. Arabidopsis thaliana, one the most members this family is SEPALLATA3 (SEP3), which involved in many aspects plant reproduction, including floral meristem and organ development. SEP3 has been shown to form homo heterooligomeric complexes with other through its intervening (I) keratin-like (K) domains. function depends on ability specific protein-protein complexes; however, atomic...
As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF's BM29 BioSAXS beamline was subject to a significant and refurbishment. In addition replacement beamline's original bending magnet source by two-pole wiggler, leading an increase in brilliance factor 60, sample environment almost completely refurbished: vacuum-compatible Pilatus3 X 2M with sensitive area 253.7 mm × 288 frame rates up 250 Hz installed, increasing active available thus q-scaling scattering images taken;...
Liquid-liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalization of macromolecules, including complex polymers such as proteins and nucleic acids, occurs a function physicochemical environment. In model plant,