Research on natural compounds is increasingly focused their effects human health. In this study, we were interested in the evaluation of nutritional value expressed as content total phenolic and antioxidant capacity new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated Department Fruit Growing Mendel University Brno. Fruits twenty one collected at onset consumption ripeness. Antioxidant capacities determined spectrometrically using DPPH•...

The study of changes nutritional value fruit during the ripening process can help estimate optimal date for harvesting to achieve best quality direct consumption and further utilization. aim this was monitor chemical composition medlar (Mespilus germanica L.) measured at five various stages including 134, 144, 154, 164 174 days after full bloom (DAFB). Fruits were analyzed ascorbic acid (AA) total phenolic compound content with respect antioxidant activity determined. In addition, selected...

Classification methods of DNA most commonly use comparison the differences in symbolic records, which requires global multiple sequence alignment. This solution is often inappropriate, causing a number imprecisions and additional user intervention for exact alignment similar segments. The segments represented as signal are characterized by shape curve. genomic signals may adjust whole sections not only individual symbols. dynamic time warping (DTW) suitable this purpose can replace sequences...

Functional foods are of interest because their significant effects on human health, which can be connected with the presence some biologically important compounds. In this study, we carried out complex analysis 239 apricot cultivars (Prunus armeniaca L.) cultivated in Lednice (climatic area T4), South Moravia, Czech Republic. Almost all previously published studies have focused only certain parameters. However, detection both primary and secondary metabolites a selection respect to...

This paper presents the utilization of progressive alignment principle for positional adjustment a set genomic signals with different lengths. The new method multiple based on dynamic time warping is tested purpose evaluating similarity length genes in phylogenetic studies. Two sets markers were used to demonstrate effectiveness evaluation intraspecies and interspecies genetic variability. part proposed modification pairwise two by using correlation sliding window. allows more accurate...

Plants respond to heavy metal toxicity in a variety of different ways including synthesis phytochelatins. The phytochelatins is catalyzed by γ-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). main aim this study was optimize high performance liquid chromatography coupled with electrochemical detector for determination phytochelatin2. optimized procedure subsequently used the mentioned molecules special attention aimed at possibility determine PC2 after activation PCS...

The aim of this study was to evaluate the bioactive substances in 19 berry cultivars edible honeysuckle (Lonicera edulis). A statistical evaluation used determine relationship between content selected and individual cultivars. Regarding mineral elements, sodium measured using potentiometry spectrophotometry. polyphenolic compounds with high antioxidant activity determined by a HPLC–UV/ED method. total amount polyphenols Folin-Ciocalteu five methods (DPPH, FRAP, ABTS, FR DMPD) that differ...

Abstract Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, functions behavior under various conditions intensively studied. In present study, two major mammalian MT isoforms (MT‐1 MT‐2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. Under optimized conditions, separation both was enabled well estimation detection limits subunits units ng per μL for MT‐2 MT‐1,...

Species delineation based on bacterial genomes is an essential part of the research prokaryotes. In silico genome-to-genome comparison methods are computationally demanding, but much less tedious and error prone than wet-lab methods. this paper, we present a novel method for genomic signal processing. The proposed uses numerical representations whole genomes, phase cumulated signal, from which four parameters derived each genome. characterize genome their calculation independent other...

The automation of a classification process electrophoresis gel images is difficult task. result highly depends on quality image digitization and imprecisions in an electrophoretic process. methodology proposed the paper helps to remove most distortions effectively overcomes problem non-uniform

Comparison and classification of organisms based on molecular data is an important task computational biology, since at least parts DNA sequences for many are available. Unfortunately, methods comparison computationally very demanding, suitable only short sequences. In this paper, we focus the redundancy genetic information stored in We proposed rules downsampling signals cumulated phase. According to length original sequence, able significantly reduce amount with slight loss information....

Visualization analysis plays an important role in metagenomics research. Proper and clear visualization can help researchers get their first insights into data by selecting different features, also revealing highlighting hidden relationships drawing conclusions. To prevent the resulting presentations from becoming chaotic, techniques have to properly tackle high dimensionality of microbiome data. Although a number methods based on reduction, correlations, Venn diagrams, network...

Low-molecular mass proteins rich in cysteines called metallothioneins (MT) can be considered as markers for the pollution of environment by metals. Here, we report on suggestion an automated procedure isolation MT followed voltammetric analysis. Primarily, optimized detection using electrochemical analyser. It was found that most sensitive and repeatable analyses are obtained at a temperature 4 °C supporting electrolyte. Further, experimental conditions antibody-linked paramagnetic...

Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity whole species has not been and it only analyzed in part for missing type strain. Here, we sequenced assembled complete strain DSM 791T, composed circular chromosome megaplasmid, used comparison with other genomes to evaluate capture evolution species. We found that WB53 HUN142 were misidentified did belong Additionally,...

Abstract Routinely used typing methods including MLST, rep-PCR and whole genome sequencing (WGS) are time-consuming, costly, often low throughput. Here, we describe a novel mini-MLST scheme for Eschericha coli as an alternative method rapid genotyping. Using the proposed scheme, 10,946 existing STs were converted into 1,038 Melting Types (MelTs). To validate new in silico analysis was performed on 73,704 strains retrieved from EnteroBase resulting discriminatory power D = 0.9465 (CI 95%...

Abstract We studied the electrochemical behaviour of adenine derivates (adenosine, 2‐aminopurine, 2,6‐diaminopurine, 6‐benzyl‐aminopurine, adenosine monophosphate, cyclic nicotinamide dinucleotide, triphosphate, S ‐adenosyl‐ L ‐methionine, and synthetic derivatives AD‐3, AD‐6 AD‐9) using flow injection analysis with detection a glassy carbon electrode. The influences pH, rate potential on signal height were tested. optimal pH was 3, mobile phase 0.75 mL min −1 1100 mV. Further, we attempted...

Large-scale comparative studies of DNA fingerprints prefer automated chip capillary electrophoresis over conventional gel planar due to the higher precision digitalization process. However, determination band sizes is still limited by device resolution and sizing accuracy. Band matching, therefore, remains key step in fingerprint analysis. Most current methods evaluate only pairwise similarity samples, using heuristically determined constant thresholds maximum allowed size deviation;...

Schlegelella thermodepolymerans is a moderately thermophilic bacterium capable of producing polyhydroxyalkanoates-biodegradable polymers representing an alternative to conventional plastics. Here, we present the first complete genome type strain S. DSM 15344 that was assembled by hybrid approach using both long (Oxford Nanopore) and short (Illumina) reads. The consists single 3,858,501-bp-long circular chromosome with GC content 70.3%. Genome annotation identified 3,650 genes in total,...

The issue of tumour cell resistance to anticancer drugs is a major problem in the treatment this grave disease and it still not satisfactorily explained. Its base lies interaction cytostatic with biomolecules synthesized by cells. One generally accepted mechanisms some metal based cytostatics overexpression metallothionein In study, electrochemical profile between 23 sulphur-rich fragments metal-binding protein cisplatin was studied. To evaluate results, constants were suggested. Here, we...

Metallothionein is intracellular metal binding protein. It can bind essential zinc ions and also toxic metals like cadmium. In cancer research the problem of resistance cells against anticancer drugs was described. this study we optimized an electrochemical method for exploring differences in signals dependence on amino acid sequence metallothionein. For purpose selected number fragments based bioinformatic processing, synthesized them made high throughput analysis. Designed analysis...