A5042293015- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Research in Cotton Cultivation
- Plant and animal studies
- Plant biochemistry and biosynthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Nematode management and characterization studies
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Insect symbiosis and bacterial influences
- Plant Reproductive Biology
- Entomopathogenic Microorganisms in Pest Control
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
- Insect Pest Control Strategies
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Biological Control of Invasive Species
- Agricultural pest management studies
- Plant Gene Expression Analysis
- Hemiptera Insect Studies
Center for Excellence in Molecular Plant Sciences
2016-2025
Chinese Academy of Sciences
2016-2025
South China Normal University
2023
University of Chinese Academy of Sciences
2018
Shanghai Institutes for Biological Sciences
2005-2017
Laboratory of Molecular Genetics
2004
Abstract The plant root cap mediates the direction of tip growth and protects internal cells. Root cells are continuously produced from distal stem cells, phytohormone auxin provides position information for organization. Here, we identify Arabidopsis thaliana response factors ARF10 ARF16, targeted by microRNA160 (miR160), as controller cell formation. Pro35S:MIR160 plants, in which expression ARF16 is repressed, arf10-2 arf16-2 double mutants display same defect, with uncontrolled division...
Arabidopsis thaliana flowers emit volatile terpenes, which may function in plant–insect interactions. Here, we report that MYC2, a basic helix-loop-helix transcription factor, directly binds to promoters of the sesquiterpene synthase genes TPS21 and TPS11 activates their expression. Expression can be induced by phytohormones gibberellin (GA) jasmonate (JA), both inductions require MYC2. The induction results increased emission sesquiterpene, especially (E)-β-caryophyllene. DELLAs, GA...
Abstract Among plant secondary metabolites terpenoids are a structurally most diverse group; they function as phytoalex‐ins in direct defense, or signals indirect defense responses which involves herbivores and their natural enemies. In recent years, more attention has been paid to the investigation of ecological role terpenoids. The biosynthesis pathways monoterpenes, sesquiterpenes, diterpenes include synthesis C5 precursor isopentenyl diphosphate (IPP) its allylic isomer dimethylallyl...
Abstract Of the two cultivated species of allopolyploid cotton, Gossypium barbadense produces extra-long fibers for production superior textiles. We sequenced its genome (AD) 2 and performed a comparative analysis. identified three bursts retrotransposons from 20 million years ago (Mya) genome-wide uneven pseudogenization peak at 11–20 Mya, which likely contributed to genomic divergences. Among 2,483 genes preferentially expressed in fiber, cell elongation regulator, PRE1 , is strikingly A t...
Abstract Immunity deteriorates with age in animals but comparatively little is known about the temporal regulation of plant resistance to herbivores. The phytohormone jasmonate (JA) a key regulator insect defense. Here, we show that JA response decays progressively Arabidopsis . We this decay regulated by miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE9 (SPL9) group proteins, which can interact ZIM-domain (JAZ) including JAZ3. As SPL9 levels gradually increase, JAZ3 accumulates and...
RNA interference (RNAi) plays an important role in regulating gene expression eukaryotes. Previously, we generated Arabidopsis and tobacco plants expressing double-stranded (dsRNA) targeting a cotton bollworm (Helicoverpa armigera) P450 gene, CYP6AE14. Bollworms fed on transgenic dsCYP6AE14 showed suppressed CYP6AE14 reduced growth gossypol-containing diet (Mao et al., Nat Biotechnol 25: 1307-1313, 2007). Here report generation analysis of dsRNA-expressing (Gossypium hirsutum) plants....
Plant reproductive organs are vulnerable to heat, but regulation of heat-shock responses in inflorescence is largely uncharacterized. Here, we report that two the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcriptional factors Arabidopsis, SPL1 and SPL12, act redundantly thermotolerance at stage. The spl1-1 spl12-1 inflorescences displayed hypersensitivity heat stress, whereas overexpression or SPL12 enhanced both Arabidopsis tobacco. RNA sequencing revealed 1939 upregulated 1479...
Abstract Cotton plants accumulate phytotoxins, including gossypol and related sesquiterpene aldehydes, to resist insect herbivores pathogens. To counteract these defensive plant secondary metabolites, cotton bollworms ( Helicoverpa armigera ) elevate their production of detoxification enzymes, cytochrome P450 monooxygenases (P450s). Besides tolerance phytotoxin, have quickly developed resistance deltamethrin, a widely used pyrethroid insecticide in field. However, the relationship between...
Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by wound-induced auxin biosynthesis. As plants age, regenerative capacity gradually decreases. How leads to burst how age wound signals collaboratively regulate poorly understood. Here, we show that increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, SPL11, suppress...
Most of the plant homeodomain-containing proteins play important roles in organ patterning and development, Arabidopsis GLABRA2 (GL2), a member class IV homeodomain-leucine zipper (HD-ZIP) proteins, is trichome non-root hair cell regulator. Here we report analysis two cotton GaHOX1 GaHOX2, isolated from diploid Gossypium arboreum. Both GaHOX2 belong to HD-ZIP family. When expressed under control GL2 promoter, rescued development an glabrous mutant gl2-2 (SALK_130213), whereas did not. On...
Significance Plants recognize insect-derived molecules and make the accurate defense response during herbivory. However, insects release effectors that disturb host plant responses for fitness. Effectors are crucial components in biotic interactions. We identified a caterpillar-derived effector (HARP1) from oral secretion of cotton bollworm, devastating agricultural pest. HARP1 is released larvae to leaves feeding able migrate wounding site into cells automatically. interacts with...
Significance Cotton is an important crop, and terpenoids form the largest group of natural products. Gossypol related sesquiterpene aldehydes in cotton function as phytoalexins against pathogens pests but pose human health concerns, oil still widely used vegetable oil. We report isolation identification four enzymes recharacterization one previously reported P450. are now close to completion gossypol pathway, progress agricultural plant sciences, data beneficial improving food safety. Among...
The miR156-targeted squamosa promoter binding protein like (SPL) transcription factors function as an endogenous age cue in regulating plant phase transition and phase-dependent morphogenesis, but the control of SPL output remains poorly understood. In Arabidopsis thaliana spatial pattern trichome is a hallmark governed by SPLs. Here, dissecting regulatory network controlling formation on stem, we show that miR171-targeted lost meristems 1 (LOM1), LOM2 LOM3, encoding GRAS family members...
<ns4:p>Acute and precise signal perception transduction are essential for plant defense against insects. Insect elicitors—that is, the biologically active molecules from insects’ oral secretion (which contains regurgitant saliva), frass, ovipositional fluids, endosymbionts—are recognized by plants subsequently induce a local or systematic response. On other hand, insects secrete various types of effectors to interfere with at multiple levels better adaptation. Jasmonate is main regulator...
ABSTRACT Verticillium wilt is a vascular disease of cotton. The causal fungus, dahliae , secretes elicitors in culture. We have generated ∼1,000 5′-terminal expressed sequence tags (ESTs) from cultured mycelium V. . A number ESTs were found to encode proteins harboring putative signal peptides for secretion, and their cDNAs isolated. Heterologous expression led the identification protein with elicitor activities. This protein, named necrosis- ethylene-inducing (VdNEP), composed 233 amino...
As sessile and autotrophic organisms, plants have evolved sophisticated pathways to produce a rich array of specialized metabolites, many which are biologically active function as defense substances in protecting from herbivores pathogens. Upon stimuli, these structurally diverse small molecules may be synthesized or constitutively accumulated. Jasmonate acids (JAs) the major phytohormone involved transducing external signals (such wounding) activate reactions, including, particular,...
Cotton cultivars have evolved to produce extensive, long, seed-born fibers important for the textile industry, but we know little about molecular mechanism underlying spinnable fiber formation. Here, report how PACLOBUTRAZOL RESISTANCE 1 (PRE1) in cotton, which encodes a basic helix-loop-helix (bHLH) transcription factor, is target gene of evolution. Differential expression homoeologous genes polyploids thought be plant adaptation and novel phenotypes. PRE1 specific cotton cells, upregulated...
Summary Caterpillar oral secretion (OS) contains active molecules that modulate plant defense signaling. We isolated an effector‐like protein (Highly Accumulated Secretory Protein 1, HAS1) from cotton bollworm ( Helicoverpa armigera ) is the most highly accumulated secretory of nondigestive components in OS and belongs to venom R‐like protein. Elimination HAS1 by plant‐mediated RNA interference reduced suppression on response plants. Plants expressing are more susceptible insect herbivory...
In Arabidopsis, the outbreaks of reactive oxygen species (ROS) occur upon pathogen recognition by pattern- and effector-triggered immunity (PTI ETI, respectively), which plays a significant role in disease resistance. Here, we found that Arabidopsis also experiences two ROS (oral secretion (OS)-induced (ROSOS)) perception OS from cotton bollworm (Helicoverpa armigera) other lepidopterans. Oral secretion-induced burst requires PTI machinery, including BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1)...
Insects and pathogens release effectors into plant cells to weaken the host defense or immune response. While imports of some bacterial fungal plants have been previously characterized, mechanisms how caterpillar enter remain a mystery. Using live cell imaging real-time protein tracking, we show that HARP1, an effector from oral secretions cotton bollworm (Helicoverpa armigera), enters via protein-mediated endocytosis. The entry HARP1 depends on its interaction with vesicle trafficking...
Abstract BACKGROUND The fall armyworm (FAW, Spodoptera frugiperda (J.E. Smith)) is a polyphagous agricultural pest with rapidly evolving adaptations to host plants. We found the oral secretion (OS) of FAW from different plants influences plant defense response differentially, suggesting its role in adapting However, protein expression profile OS respond largely unknown. RESULTS Here, mass spectrometry assay, we identified total 256 proteins fed on cotton ( Gossypium hirsutum L.), tobacco...