A5078267220- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Blood properties and coagulation
- Caveolin-1 and cellular processes
- Pluripotent Stem Cells Research
- Polymer Surface Interaction Studies
- Neonatal Respiratory Health Research
- Mechanical and Optical Resonators
- Advanced Polymer Synthesis and Characterization
- Microtubule and mitosis dynamics
- Inhalation and Respiratory Drug Delivery
- Nanopore and Nanochannel Transport Studies
- Molecular Junctions and Nanostructures
- Mathematical Biology Tumor Growth
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Advanced Materials and Mechanics
- Optical Coherence Tomography Applications
- Micro and Nano Robotics
- Innovative Microfluidic and Catalytic Techniques Innovation
Centre National de la Recherche Scientifique
2016-2025
Université de Bordeaux
2016-2025
Laboratoire Photonique, Numérique et Nanosciences
2014-2025
Institut d’Optique Graduate School
2016-2025
University of Geneva
2016
Charles Humbert 8
2016
Physique des Cellules et Cancers
2005-2014
Institut Curie
2005-2014
Délégation Paris 6
2013
Chimie ParisTech
2012-2013
Significance Tumor growth intrinsically generates pressure onto the surrounding tissues, which conversely compress tumor. These mechanical forces have been suggested to contribute tumor regulation. We developed a microfluidic technique produce 3D cell-based assays and interrogate interplay between mechanics in vitro. Multicellular spheroids are grown permeable elastic capsules. Capsule deformation provides direct measure of exerted pressure. By simultaneously imaging spheroid by confocal...
Sorting of lipids and proteins is a key process allowing eukaryotic cells to execute efficient accurate intracellular transport maintain membrane homeostasis. It occurs during the formation highly curved intermediates that shuttle between cell compartments. Protein sorting reasonably well described, but lipid much less understood. Lipid has been proposed be mediated by physical mechanism based on coupling composition high curvature intermediates. To test this hypothesis, we have performed...
The generation of membrane curvature in intracellular traffic involves many proteins that can curve lipid bilayers. Among these, dynamin-like were shown to deform membranes into tubules, and thus far are the only known mechanically drive fission. Because dynamin forms a helical coat circling tubule, its polymerization is thought be responsible for this deformation. Here we show force generated by polymerization, 18 pN, sufficient yet still counteracted high tension. Importantly, observe at...
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Polymersomes, which are stable and robust vesicles made of block copolymer amphiphiles, good candidates for drug carriers or micro/nanoreactors. Polymer chemistry enables almost unlimited molecular design responsive polymersomes whose degradation upon environmental changes has been used the slow release active species. Here, we propose a strategy to remotely trigger instantaneous polymersome bursting. We have designed asymmetric polymer vesicles, in only one leaflet is composed polymers. In...
A 3D printed chip for the production of neuron-containing alginate capsules.
Many cell movements proceed via a crawling mechanism, where polymerization of the cytoskeletal protein actin pushes out leading edge membrane. In this model, membrane tension has been seen as an impediment to filament growth and motility. Here we use simple model motility, Caenorhabditis elegans sperm cell, test how affects movement cytoskeleton dynamics. To enable these analyses, create transgenic worm strains carrying with fluorescently labeled cytoskeleton. Via osmotic shock deoxycholate...
Many organs are formed through folding of an epithelium. This change in shape is usually attributed to tissue heterogeneities, for example, local apical contraction. In contrast, compressive stresses have been proposed fold a homogeneous epithelium by buckling. While buckling appealing mechanism, demonstrating that it underlies requires measurement the stress field and material properties tissue, which currently inaccessible vivo. Here, we show monolayers identical cells proliferating on...
Human pluripotent stem cells (hPSCs) have emerged as the most promising cellular source for cell therapies. To overcome scale-up limitations of classical 2D culture systems, suspension cultures been developed to meet need large-scale in regenerative medicine. Despite constant improvements, current protocols that use microcarriers or generate aggregates only achieve moderate amplification performance. Here, guided by reports showing hPSCs can self-organize vitro into cysts reminiscent...
Recent advances in bioengineering have made it possible to develop increasingly complex biological systems recapitulate organ functions as closely vitro . Monitoring the assembly and growth of multi-cellular aggregates, micro-tissues or organoids extracting quantitative information is a crucial but challenging task required decipher underlying morphogenetic mechanisms. We present here an imaging platform designed be accommodated inside incubator which provides high-throughput monitoring cell...
We discuss the pulling force f required to extrude a lipid tube from living cell as function of extrusion velocity L. The main feature is membrane friction on cytoskeleton. As recently observed for neutrophils, tether exhibits "shear thinning" response over large range velocities, which was previously interpreted by assuming viscoelastic flows sliding membrane. Here, we propose an alternative explanation based purely Newtonian flow: diameter decreases concomitantly with increase tension in...
We have demonstrated that the two- and three-dimensional motility of human pathogenic parasite Entamoeba histolytica (Eh) depends on sustained instability intracellular hydrostatic pressure. This drives cyclic generation healing membrane blebs, with typical protrusion velocities 10-20 μm/second over a few hundred milliseconds times 10 seconds. The use novel micro-electroporation method to control pressure enabled us develop qualitative model three parameters: rate myosin-driven internal...
One of main challenges in light-sheet microscopy is to design the as extended and thin possible--extended cover a large field view, optimize resolution contrast. However, decrease beam's waist also decreases illumination depth field. Here, we introduce new kind beam that call sectioned Bessel beam. These beams can be generated by blocking opposite sections angular spectrum. In combination with confocal-line detection optical sectioning performance decoupled from By simulations experiments...
Most achievements to engineer blood vessels are based on multiple-step manipulations such as manual sheet rolling or sequential cell seeding followed by scaffold degradation. Here, we propose a one-step strategy using microfluidic coextrusion device produce mature functional vessels. A hollow alginate hydrogel tube is internally coated with extracellular matrix direct the self-assembly of mixture endothelial cells (ECs) and smooth muscle (SMCs). The resulting vascular structure has correct...
Abstract Caveolin-3 is the major structural protein of caveolae in muscle. Mutations CAV3 gene cause different types myopathies with altered membrane integrity and repair, expression muscle proteins, regulation signaling pathways. We show here that myotubes from patients bearing P28L R26Q mutations present a dramatic decrease at plasma membrane, resulting abnormal response to mechanical stress. Mutant are unable buffer increase tension induced by This results impaired IL6/STAT3 pathway...