A5001018161- Cholesterol and Lipid Metabolism
- Fatty Acid Research and Health
- Lipid metabolism and biosynthesis
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer, Lipids, and Metabolism
- Lipoproteins and Cardiovascular Health
- Peroxisome Proliferator-Activated Receptors
- Cellular transport and secretion
- Atherosclerosis and Cardiovascular Diseases
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Antioxidant Activity and Oxidative Stress
- Drug Transport and Resistance Mechanisms
- Acute Myocardial Infarction Research
- Lipid Membrane Structure and Behavior
- Cardiac Imaging and Diagnostics
- Free Radicals and Antioxidants
- SARS-CoV-2 detection and testing
- Neurological and metabolic disorders
- Oral microbiology and periodontitis research
- Vitamin C and Antioxidants Research
- Alcoholism and Thiamine Deficiency
- Apelin-related biomedical research
- Metabolomics and Mass Spectrometry Studies
- Hormonal Regulation and Hypertension
Hôpital Européen Georges-Pompidou
2014-2024
Assistance Publique – Hôpitaux de Paris
2014-2024
Hôpital Européen
2013-2024
Université Paris Cité
2006-2023
Sorbonne Paris Cité
2022
Laboratoire de Biochimie Théorique
2008-2016
Université Paris-Sud
2013
Institut Galien Paris-Saclay
2013
Inserm
1998-2008
Sorbonne Université
2008
Abstract St AR ‐related lipid transfer domain‐3 ( STARD 3) is a sterol‐binding protein that creates endoplasmic reticulum ER )–endosome contact sites. How this protein, at the crossroad between sterol uptake and synthesis pathways, impacts intracellular distribution of was ill‐defined. Here, by using in situ cholesterol labeling quantification, we demonstrated 3 induces accumulation endosomes expense plasma membrane. 3‐mediated routing depends both on its activity ability to create –endosome...
Despite the progress of anti-cancer treatment, prognosis many patients with solid tumors is still dismal. Reliable noninvasive biomarkers are needed to predict patient survival and therapy response. Here, we propose a Humoral Complementomics approach: work-up assays comprehensively evaluate complement proteins, activation fragments, autoantibodies targeting proteins in plasma, which correlated intratumoral activation, and/or local production, focusing on localized metastatic clear cell renal...
The small GTPases of the Rab family act as a molecular switch regulating various aspects membrane trafficking through selective recruitment effector proteins. Whereas Rab7 has been classically involved in regulation transport within endolysosomal network, persistent controversy remains to whether also plays role earlier steps endosomal trafficking. In this study, we show that depletion or inactivation results enlargement both early and late endosomes. led retention significant fraction...
Abstract By using fast protein liquid chromatography, we isolated from human plasma a minor electronegative LDL subfraction designated LDL(−). After immunoaffinity chromatography against apolipoprotein (apo)(a) and apo A-I, LDL(−) represented 6.7±0.9% (mean±SD; n=18) of total LDL. Compared with the major subfraction, LDL(+), contained similar amounts thiobarbituric acid–reactive substances, conjugated dienes, vitamin E had lipid/protein ratio mean density. Moreover, B was not aggregated its...
Abstract Background Humoral response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs within the first weeks after disease 2019 (COVID-19). Those antibodies exert a neutralizing activity against SARS-CoV-2, whose evolution over time COVID-19 as well efficiency novel variants are poorly characterized. Methods In this prospective study, sera of 107 patients hospitalized with were collected at 3 and 6 months postinfection. We performed quantitative neutralization...
Objective— Therapeutic strategies to raise low plasma HDL-cholesterol levels, with concomitant normalization of the intravascular metabolism, physicochemical properties, and antiatherogenic function HDL particles, are a major focus in atherosclerosis prevention. Methods Results— Patients displaying Type IIB hyperlipidemia (n=14) healthy controls (n=11) were recruited. After drug washout, dyslipidemic patients first received atorvastatin (10 mg/d) for 6 weeks subsequently...
Background: Coronavirus disease 2019 (COVID-19) has been associated with cardiovascular complications and coagulation disorders. Objectives: To explore clinical biological parameters of COVID-19 patients hospitalization criteria that could predict referral to intensive care unit (ICU). Methods: Analyzing the profiles at admission. Results: Among 99 consecutive fulfilled for hospitalization, 48 were hospitalized in medicine department, 21 first admitted ward department referred later ICU, 30...
Carmat bioprosthetic total artificial heart (Aeson; A-TAH) is a pulsatile and autoregulated device. The aim of this study to evaluate level hemolysis potential acquired von Willebrand syndrome after A-TAH implantation.We examined the presence in adult patients receiving support (n=10) during their whole clinical follow-up comparison with control subjects Heartmate II or III support. We also performed fluid structure interaction model coupled computational dynamics simulation resulting shear...
We describe a methodology develpped to separate different forms of charge-modified low density lipoproteins (LEL) using the fast protein liquid chromatography (FPLC) system from Pharmacia.Lipoproteins were isolated by sequential ultracentrifugation and introduced onto an anion-exchange column (Mono Q HR 515).The multistep NaCl gradient elution was optimized analytical variables determined on copper-oxidized LDL.After oxidation copper for various 'Preliminary results this work presented at 55th Annual
Intracellular transport of cholesterol contributes to the regulation cellular homeostasis by mechanisms that are yet poorly defined. In this study, we characterized impact dynasore, a recently described drug specifically inhibits enzymatic activity dynamin, GTPase regulating receptor endocytosis and trafficking. Dynasore strongly inhibited uptake low-density lipoprotein (LDL) in HeLa cells, lower extent human macrophages. both cell types, dynasore treatment led abnormal accumulation LDL free...
Cholesterol is one of the most essential membrane components in mammalian cells and plays a critical role several cellular functions. It now established that intracellular cholesterol transport contributes to regulation homeostasis by mechanisms are yet poorly defined. In this study, we examined clathrin- dynamin-dependent trafficking on regulatory machinery involved homeostasis. Thus, expression levels three major sterol-sensitive genes, sterol-regulatory element binding protein 2...
Abstract Leber’s hereditary optic neuropathy (LHON) is the most common disorder due to mitochondrial DNA mutations and complex I deficiency. It characterized by an acute vision loss, generally in young adults, with a higher penetrance males. How dysfunction induces peculiar LHON clinical presentation remains unanswered question. To gain insight into this question, we carried out non-targeted metabolomic investigation using plasma of 18 patients, during chronic phase disease, comparing them...
Persistent patency of the arterial duct is one most common congenital cardiac malformations. We recently showed that mutations in MYH11 gene result a disease combining familial thoracic aortic aneurysm and dissection, along with duct. It also known smooth muscle myosin heavy chain involved physiological closure With this mind, we investigated whether was susceptibility for sporadic occurrence isolated persistent sequenced entire coding sequence 60 Caucasian children born after 36 weeks...