A5066798868- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Ion channel regulation and function
- Exercise and Physiological Responses
- Peripheral Neuropathies and Disorders
- Vestibular and auditory disorders
- Neurogenetic and Muscular Disorders Research
- Biochemical effects in animals
- Mesenchymal stem cell research
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Lipid Membrane Structure and Behavior
- DNA and Nucleic Acid Chemistry
- Healthcare and Venom Research
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Nitric Oxide and Endothelin Effects
- Glycosylation and Glycoproteins Research
- Pharmacological Receptor Mechanisms and Effects
- Galectins and Cancer Biology
- Advanced biosensing and bioanalysis techniques
- Cardiac electrophysiology and arrhythmias
- Medicinal Plants and Bioactive Compounds
- 3D Printing in Biomedical Research
University of Alberta
2020-2025
Women and Children’s Health Research Institute
2020-2025
MacEwan University
2022
In the peripheral nervous system, nociceptors become hyperexcitable in both acute and chronic pain conditions. This phenotype can be mediated by dysregulated calcium, which occurs if endoplasmic reticulum (ER) mitochondria fail to buffer it appropriately. The redox enzyme oxidoreductin 1 (ERO1) regulates calcium transfer at ER-mitochondria contact sites (ERMCS). this study we hypothesized that inhibiting ERO1 thereby dampening ERMCS might lower nociceptor hyperexcitability sensory neurons...
A previous study has demonstrated the benefit of modification polyethylenimine (PEI1.2k) by lipids through a p-hydroxyphenylacetic acid (PHPA) linker and polyanion (PA), which is now extended in this report to several primary cells. The formulated binary (lipopolymer/NAs) ternary (lipopolymer/NAs/PA) complexes displayed no significant toxicity (MTT/hemolysis assay). pDNA/mRNA with PEI1.2k-PHPA-Lin9 PEI1.2k-PHPA-Lau5-Ole5 lipopolymers showed gene expression levels higher than those other...
Gangliosides are glycosphingolipids that play an integral role in cell signaling and provide neuroprotection. While present on extracellular vesicles (EVs), key mediators of intercellular communication, their EV biogenesis remains unclear. Here, we identify gangliosides, both endogenously synthesized exogenously administered, as modulators biogenesis, with the specific composition glycan headgroup presence or absence sialic acid N-acetyl-D-galactosamine residues dictating whether they...
Dorsal root ganglia (DRGs) are peripheral structures adjacent to the dorsal horn of spinal cord, which house cell bodies sensory neurons as well various other types. Published culture protocols often refer whole dissociated DRG cultures being neuronal, despite presence fibroblasts, Schwann cells, macrophages, and lymphocytes. While these sufficient for imaging applications where can be discerned based on morphology or staining, protein RNA homogenates collected from not primarily neuronal in...
Abstract Multiple Sclerosis (MS) is an autoimmune disease with notable sex differences. Women are not only more likely to develop MS but also than men experience neuropathic pain in the disease. It has been postulated that can originate peripheral nervous system at level of dorsal root ganglia (DRG), which houses primary sensing neurons (nociceptors). These nociceptors become hyperexcitable response inflammation, leading sensitization and eventually central sensitization, maintains...
ABSTRACT Kv1.2 potassium channels influence excitability and action potential propagation in the nervous system. Unlike closely-related Kv1 channels, exhibits highly variable voltage-dependence of gating, attributed to regulation by unidentified extrinsic factors. Variable gating is strongly influenced extracellular redox potential, we demonstrate that currents dorsal root ganglion sensory neurons exhibit similar variability sensitivity as observed when channel heterologously expressed cell...
Kv1.2 potassium channels influence excitability and action potential propagation in the nervous system. Unlike closely-related Kv1 channels, exhibits highly variable voltage-dependence of gating, attributed to regulation by unidentified extrinsic factors. Variability gating is strongly influenced extracellular redox potential, we demonstrate that currents dorsal root ganglion sensory neurons exhibit similar variability sensitivity as observed when channel heterologously expressed cell lines....
Abstract Background. The intricate interplay between inflammation, its effects on neuronal excitability, and the ensuing plasticity of dorsal root ganglion (DRG) sensory neurons remains to be fully explored. In this study, we have investigated impact inflammatory conditioned media excitability activity DRG how relates subsequent growth responses these cells. Findings. We show here that an early phase increased in response is critical for engagement plastic processes, profiles are linked...
ABSTRACT The complex relationship between inflammation, its effects on neuronal excitability and the ensuing plasticity of dorsal root ganglion (DRG) sensory neurons remains to be fully explored. In this study, we have employed a system experiments assessing impact inflammatory conditioned media derived from activated immune cells activity DRG how relates subsequent growth responses these cells. We show here that an early phase increased in response is critical for engagement plastic...
Dorsal root ganglia (DRGs) are peripheral structures adjacent to the dorsal horn of spinal cord, which house cell bodies sensory neurons as well various other types. Published culture protocols often refer whole dissociated DRG cultures being neuronal, despite presence fibroblasts, Schwann cells, macrophages, and lymphocytes. While these sufficient for imaging applications where can be discerned based on morphology or staining, protein RNA homogenates collected from not primarily neuronal in...
Abstract Multiple Sclerosis (MS) is an autoimmune disease with notable sex differences. Women are not only more likely to develop MS but also than men experience neuropathic pain in the disease. It has been postulated that can originate peripheral nervous system at level of dorsal root ganglia (DRG), which houses primary sensing neurons (nociceptors). These nociceptors become hyperexcitable response inflammation, leading sensitization and eventually central sensitization, maintains...
Abstract Multiple Sclerosis (MS) is an autoimmune disease with notable sex differences. Women are not only more likely to develop MS but also than men experience neuropathic pain in the disease. It has been postulated that can originate peripheral nervous system at level of dorsal root ganglia (DRG), which houses primary sensing neurons (nociceptors). These nociceptors become hyperexcitable response inflammation, leading sensitization and eventually central sensitization, maintains...