A5085689534- Blood groups and transfusion
- NF-κB Signaling Pathways
- Platelet Disorders and Treatments
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Blood disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- RNA regulation and disease
- Reproductive System and Pregnancy
- T-cell and Retrovirus Studies
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- Estrogen and related hormone effects
- Cancer Research and Treatments
- Vector-Borne Animal Diseases
- Blood transfusion and management
- Dermatology and Skin Diseases
- Inflammatory mediators and NSAID effects
- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Systemic Lupus Erythematosus Research
The University of Tokyo
1996-2025
Tottori University
2025
Walter and Eliza Hall Institute of Medical Research
2020-2024
The University of Melbourne
2020-2024
Sapporo Kosei General Hospital
2024
Meijo University
2020
Sakai Municipal Hospital
2019
Tokyo University of Science
1999-2015
University of the Ryukyus
2011
Kitasato University Hospital
2011
Epidemiological studies have demonstrated that nonsteroidal anti-inflammatory drugs (NSAIDs), known to inhibit cyclooxygenase (COX), reduce the risk of colorectal cancer. COX is a key enzyme in prostaglandin biosynthesis, and two isoforms COX, COX-1 COX-2, been identified. Recently COX-2 has reported frequently overexpress neoplasms play role tumorigenesis tumour progression. In this study, using immunohistochemistry, we examined expression advanced human cancer its correlation with...
OTU deubiquitinase with linear linkage specificity (OTULIN) regulates inflammation and cell death by deubiquitinating ubiquitin chains generated the chain assembly complex (LUBAC). Biallelic loss-of-function mutations causes OTULIN-related autoinflammatory syndrome (ORAS), while OTULIN haploinsuffiency has not been associated spontaneous inflammation. However, herein, we identify two patients heterozygous mutation p.Cys129Ser in OTULIN. Consistent ORAS, observed accumulation of chains,...
It is proposed that non-steroidal anti-inflammatory drugs (NSAIDs) reduce colorectal tumorigenesis by inhibition of cyclooxygenase (COX). COX a key enzyme in the conversion arachidonic acid to prostaglandins and two isoforms have been characterized, COX-1 COX-2. Multiple studies shown COX-2 expressed at high levels tumours play role tumorigenesis. Recently it has reported selective inhibits colon cancer cell growth. In this study we investigated effect inhibitor (JTE-522) on haematogenous...
Polymorphism of glycoprotein IIIa on human platelets is one the factors in alloimmunization that causes neonatal alloimmune thrombocytopenia and refractoriness to platelet transfusion.DNA typing methods were originally developed determine genotypes five antigen (HPA) systems located IIIa: HPA-1, HPA-4, HPA-6W, HPA-7W HPA-8W. The gene frequencies these antigens determined by DNA 331 unrelated Japanese donors.The low-frequency 0.002, 0.011, 0.027 for HPA-1b, HPA-4b, HPA-6W(b), respectively....
The Tax protein of human T-cell leukemia virus type 1 (HTLV-1) is crucial for the development adult (ATL), a highly malignant CD4+ T cell neoplasm. Among multiple aberrant Tax-induced effects on cellular processes, persistent activation transcription factor NF-κB, which activated only transiently upon physiological stimulation, essential leukemogenesis. We and others have shown that induces IκB kinase (IKK) complex, critical step in NF-κB activation, by generating Lys63-linked polyubiquitin...
The recent possible neurodevelopmental etiology of schizophrenia makes the neurotrophin‐3 (NT‐3) gene an interesting candidate locus. We studied allelic distributions dinucleotide repeat polymorphism at NT‐3 locus in 70 patients with and controls. A highly significant difference between two groups was observed allele A3. Even Bonferroni's correction used, still significant. Individuals homozygous or heterozygous for A3 had a 2.4‐fold increased risk schizophrenia. Determination genotype may...
Protein aggregates are degraded by both the autophagy–lysosomal and ubiquitin–proteasome pathways. Macroautophagy microautophagy, two forms of pathway, widely conserved across eukaryotes. While macroautophagy has been extensively studied in context degradation protein aggregates, microautophagy remains less explored. Here, we identify UBAP1-containing ESCRT-I complex PTPN23 as new regulators for aggregated proteins through an unbiased genome-wide CRISPR knockout screen, using a cell line...
Pyogenic spondylitis typically presents with back and neck pain, fever, fatigue. Sharp chest pain upon deep breathing, commonly associated pleurisy, is not a typical manifestation of pyogenic spondylitis. We report case pleuritic as the initial presentation A 62-year-old male patient presented one-week history right-sided fever. Chest computed tomography (CT) revealed right pleural effusion. The was initially treated for bacterial pleurisy without improvement subsequently developed pain....
Abstract Despite various inhibitors targeting the zinc center(s) of enzymes, drugs that target fingers have not been examined in detail. We previously developed a dithiol compound named SN‐1 has an inhibitory effect on function finger transcription factors, but its mechanism action yet elucidated. To establish general principle for new drugs, details against protein were examined. As zinc‐finger‐containing protein, we focused TRAF6, which is related to cancer and inflammation. Binding TRAF6...
Summary Employing an immunoblotting procedure, we have identified and characterized autoantigen carried on glycoprotein (GP) IIb in a patient with chronic idiopathic thrombocytopenic purpura (ITP), compared the location of that platelet‐specific alloantigen Bak . Immunoblots, using partially purified GP IIb/IIIa complex as target antigen, indicated IIIα both ITP alloantigen. The plasma contained another antibody against 100 kD protein (P100), trace contaminant IIb/ IIIa sample, which is...
Biallelic loss of function mutations in the linear chain specific deubiquitinase (DUB) OTULIN (OTU Deubiquitinase With Linear Linkage Specificity) result Related Autoinflammatory Syndrome (ORAS). To date all reported ORAS patients have had homozygous or compound heterozygous mutations, however we identified a patient with monoallelic mutation p.Cys129Ser. Consistent phenotype, observed accumulation ubiquitin chains, increased sensitivity to TNF induced cell death and dysregulation...
In order to further clarify the diversity of HLA‐B15 antigens and correspondence serological types with alleles in Asians, we screened various B15 splits by means a polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) method. Subsequently, genes encoding variants were sequenced. Two novel alleles, B*1528 B*1529, identified: nucleotide sequence former contained single‐base substitution at position 263 exon 2 as compared that B*1501 allele, which results an amino acid...
Abstract. The glycoprotein (GP) localization of a new platelet‐specific allo‐antigen Yuk b is described. antibody was isolated from serum patient with neonatal allo‐immune thrombocytopenia. In immunoblot procedure, it bound exclusively to platelet GP IIIa, like anti‐Pl A1 , while the known anti‐Bak and anti‐Lek reacted IIb. Analysis chymotrypsin‐treated platelets anti‐Yuk revealed no binding in 68‐kilodalton position did. Thus, unlike P1 antigen, determinant either resides on 30‐kilodalton...
Journal Article Activation of the IκB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination Get access Yuri Shibata, Shibata 1Department Cancer Biology, Division Cellular and Molecular Institute Medical Science, University Tokyo, Shirokane-dai, Minato-ku, Tokyo 108-8639; 2Department Immunology, Faculty Medicine, Ryukyus, Nishihara, Okinawa 903-0213, Japan Search for other works this author on: Oxford Academic PubMed Google Scholar Yuetsu Tanaka,...
Abstract Coronaviruses, including SARS-CoV-2, encode multifunctional proteases that are essential for viral replication and evasion of host innate immune mechanisms. The papain-like protease PLpro cleaves the polyprotein, reverses inflammatory ubiquitin anti-viral ubiquitin-like ISG15 protein modifications 1,2 . Drugs target SARS-CoV-2 (hereafter, SARS2 PLpro) may hence be effective as treatments or prophylaxis COVID-19, reducing load reinstating responses 3 We here characterise in molecular...
Background and Objectives Morbidity mortality from ABO‐incompatible transfusion persist as consequences of human error. Even so, insufficient attention has been given to improving safety within the hospital. Materials Methods National surveys blood transfusions were conducted by Japanese Society Blood Transfusion, with support Ministry Health, Labor Welfare. Surveys concluded in 2000 2005 analysed data previous 5 years (January 1995 December 1999 January 2004, respectively). The first survey...