A5021128260- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Reproductive tract infections research
- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced MRI Techniques and Applications
- Biochemical and Molecular Research
- Galectins and Cancer Biology
- Urinary Tract Infections Management
- Developmental Biology and Gene Regulation
- Amino Acid Enzymes and Metabolism
- Insect and Arachnid Ecology and Behavior
- Molecular spectroscopy and chirality
- Cerebrovascular and genetic disorders
- Hepatitis B Virus Studies
- Phosphodiesterase function and regulation
- Force Microscopy Techniques and Applications
- Escherichia coli research studies
- Advanced Electron Microscopy Techniques and Applications
- Biological Activity of Diterpenoids and Biflavonoids
- Synthesis and Biological Activity
- Antimicrobial Peptides and Activities
- Insect Utilization and Effects
Louisiana State University
2012-2024
John Carroll University
2024
University of Georgia
2005-2008
Georgia State University
2008
Purdue University West Lafayette
1999-2004
National Taiwan University
2003
Tulane University
2002
Mutations in rumi result a temperature-sensitive loss of Notch signaling Drosophila . Rumi is soluble, endoplasmic reticulum-retained protein with CAP10 domain that functions as O -glucosyltransferase. In human and mouse genomes, three potential homologues exist: one high degree identity to (52%), two others lower degrees but including (KDELC1 KDELC2). Here we show both Rumi, not KDELC1 or KDELC2, catalyze transfer glucose from UDP-glucose an EGF repeat factor VII. Similarly, rescues the...
Members of the Pht1 family plant phosphate (Pi) transporters play vital roles in Pi acquisition from soil and planta translocation to maintain optimal growth development. The study specificities biochemical properties will contribute improving current understanding phosphorus homeostasis use-efficiency. In this study, we show through split vivo interaction methods vitro analysis microsomal root tissues that Arabidopsis thaliana Pht1;1 Pht1;4 form homomeric heteromeric complexes. Transient...
An automated method for high-throughput nuclear magnetic resonance (NMR) spectroscopy has been developed using a four-coil Multiplex NMR probe. The probe is constructed with solenoidal microcoils optimized detection of small volume, mass-limited samples and flow-through design. Four can be simultaneously injected into the robotics liquid handler then analyzed in rapid succession selective excitation experiment. Due to simultaneous injection four reduced analysis time excitation, rate...
Phosphodiesterase 4 catalyzes the hydrolysis of cyclic AMP and is a target for development anti-inflammatory agents. We have designed synthesized series phenyl alkyl ketones as PDE4 inhibitors. Among them, 13 compounds were identified having submicromolar IC50 values. The most potent values in mid- to low-nanomolar range. Compound 5v also showed preference with selectivity >2000-fold over PDE7, PDE9, PDE2, PDE5. Docking 5v, 5zf, 5za into binding pocket catalytic domain revealed similar...
Glycans that are either N-linked to asparagine or O-linked serine threonine the hallmark of glycoproteins, a class protein dominates mammalian proteome. These glycans perform important functions in cells and some cases required for activity. Nuclear magnetic resonance (NMR) spectroscopy is powerful tool studying glycan structure interactions, particularly form exploits heteronuclei such as 13C. Here an approach presented uses α-2,6-sialyltransferase (ST6Gal-I) enzymatically add...
Two improved approaches for the rapid analysis of multiple samples using multiplex sample NMR are described. In first approach, frequency-selective 90 degrees radio frequency pulses and large pulsed field gradients applied to excite detect in succession. This method is advantageous with relatively long longitudinal (T1) relaxation times. second chemical shift imaging acquire both spectral spatial information simultaneously. Chemical more time-consuming than selective excitation; however, it...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTZeolite-Coated Optical Microfibers for Intrazeolite Photocatalysis Studied by in Situ Solid-State NMRAjit R. Pradhan, Megan A. Macnaughtan, and Daniel RafteryView Author Information H. C. Brown Laboratory, Department of Chemistry Purdue University, West Lafayette, Indiana 47907-1393 Cite this: J. Am. Chem. Soc. 2000, 122, 2, 404–405Publication Date (Web):December 31, 1999Publication History Received29 July 1999Published online31 December...
Abstract The traditional NMR‐based method for determining oligomeric protein structure usually involves distinguishing and assigning intra‐ intersubunit NOEs. This task becomes challenging when symmetric homo‐dimer structures because NOE cross‐peaks from a given pair of protons occur at the same position whether or in origin. While there are isotope‐filtering strategies intra intermolecular interactions these cases, they laborious often prove ineffectual cases weak dimers, where observation...
Reductive 13C-methylation of proteins has been used as an isotope labeling strategy to study protein structure, function, and dynamics by nuclear magnetic resonance (NMR) spectroscopy. However, assigning the resulting 13C-dimethylamine peaks in a 1H-13C NMR spectrum proved be difficult, but it is important expand scope method. The assignment presented here utilizes mass spectrometry (MS) for sequence identification varying 13C/12C ratios correlate with data. site-specific reactivity lysines...
Coptotermes formosanus is an imported, subterranean termite species with the largest economic impact in United States. The frontal glands of soldier caste termites comprising one third body mass, contain a secretion expelled through foramen defense. small molecule composition gland well-characterized, but proteins remain to be identified. Herein reported structure and function several found defense secretion. TFP4 6.9 kDa, non-classical group 1 Kazal-type serine protease inhibitor activity...
Chlamydia trachomatis Scc4 (formerly CT663) engages the transcription machinery and pathogenic type III secretion system (T3SS). Both machines are required for infection. These requirements limited ability genetic manipulation in have hampered dissection of Scc4's contributions. Here, by developing bacterial systems that permit controlled expression stable maintenance Scc4, we assess effects on chlamydial growth phenotype, secretion, patterns T3SS gene expression. Expressing Escherichia coli...
Samples of zeolites supported on optical microfibers were prepared with tetraethyl orthosilicate (TEOS) as a binding agent using sol−gel methods. A slower rate TEOS hydrolysis in the relative to silanol condensation was observed be crucial for successful support zeolite particles fibers. The amount water and pH reaction gel varied control optimize rates condensation. Scanning electron microscopy (SEM), Brunauer−Emmett−Teller (BET) surface area measurements, thermogravimetric analysis (TGA)...
Peptides that exhibit enzymatic or hormonal activities are regulatory factors and desirable therapeutic drugs because of their high target specificity minimal side effects. Unfortunately, these susceptible to degradation, leading rapid elimination thereby demanding frequent dosage. Structurally modified forms some peptide have shown enhanced pharmacokinetics, improving oral bioavailability. Here, we discuss a novel glycomimetic approach modify lysine residues in peptides. In model system,...
Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes the most common sexually transmitted disease in world. The bacterium has a unique biphasic developmental cycle with type III secretion system (T3SS) to invade host cells. Scc4 class I T3SS chaperone forming heterodimer complex Scc1 essential virulence effector, CopN. also functions as RNA polymerase binding protein regulate σ66-dependent transcription. Aggregation and low solubility of 6X-histidine-tagged...