A5103239945- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- DNA Repair Mechanisms
- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Cancer therapeutics and mechanisms
- Metal complexes synthesis and properties
- Autophagy in Disease and Therapy
- Protein Interaction Studies and Fluorescence Analysis
- RNA modifications and cancer
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
Max Planck Institute of Biochemistry
2018-2019
Saha Institute of Nuclear Physics
2007-2015
Eukaryotic DNA is compacted in the form of chromatin, a complex with histones and other non-histone proteins. The intimate association chromatin raises possibility that DNA-interactive small molecules may bind to chromatin-associated proteins such as histones. Employing biophysical biochemical techniques we have characterized interaction classical intercalator, ethidium bromide (EB) its structural analogue propidium iodide (PI) hierarchical genomic components: long chromatosome, core octamer...
Proteasomes occur in all three domains of life, and are the principal molecular machines for regulated degradation intracellular proteins. They play key roles maintenance protein homeostasis, control vital cellular processes. While eukaryotic 26S proteasome is extensively characterized, its putative evolutionary precursor, archaeal proteasome, remains poorly understood. The primordial consists a 20S proteolytic core particle (CP), an AAA-ATPase module. This minimal complex degrades...
Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, when combined with focused ion beam (FIB) milling, macromolecules be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, yields sub-nanometer in situ STA averages.
Background Distamycin A is a prototype minor groove binder, which binds to B-form DNA, preferentially at A/T rich sites. Extensive work in the past few decades has characterized binding level of double stranded DNA. However, effect same on physiological i.e. DNA complexed chromatin, not been well studied. Here we elucidate from structural perspective, interaction distamycin with soluble isolated Sprague-Dawley rat. Methodology/Principal Findings Chromatin hierarchical assemblage and protein....
The aqueous-phase self-association of mithramycin (MTR), an aureolic acid anticancer antibiotic, has been studied using different spectroscopic techniques such as absorption, fluorescence, circular dichroism, and 1H nuclear magnetic resonance spectroscopy. Results from these studies indicate the anionic antibiotic at pH 8.0 over a concentration range micromolar to millimolar. These results could be ascribed following steps self-association: M + left arrow right M2, M2 M3, M3 M4, where M, M4...
The condensed structure of chromatin limits access cellular machinery towards template DNA. This in turn represses essential processes like transcription, replication, repair and recombination. repression is alleviated by a variety energy dependent processes, collectively known as “chromatin remodeling”. In eukaryotic cell, fine balance between de-condensed states helps to maintain an optimum level gene expression. DNA binding small molecules have the potential perturb such equilibrium. We...
Abstract Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) can resolve protein complexes at near atomic resolution, when combined with focused ion beam (FIB) milling, macromolecules be observed within their native context. Unlike single particle acquisition (SPA), cryo-ET slow, which may reduce overall project throughput. We here propose a fast, multi-position tomographic scheme based on beam-tilt corrected beam-shift imaging along the tilt axis, yields sub-nanometer in situ...