A5085672260- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- 3D Printing in Biomedical Research
- Complex Systems and Time Series Analysis
- EEG and Brain-Computer Interfaces
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Chromatin Remodeling and Cancer
- Neural Networks and Applications
- Neurological disorders and treatments
- Photoreceptor and optogenetics research
- Time Series Analysis and Forecasting
- Fractal and DNA sequence analysis
- Advanced Memory and Neural Computing
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Gene Regulatory Network Analysis
Italian Institute of Technology
2020-2023
University of Genoa
2023
University of Trento
2019-2020
Medulloblastoma (MB) is the most common malignant brain tumor in children and among subtypes, Group 3 MB has worst outcome. Here, we perform an vivo, patient-specific screen leading to identification of Otx2 c-MYC as strong inducers. We validated our findings human cerebellar organoids where Otx2/c-MYC give rise MB-like harboring a DNA methylation signature that clusters with tumors. Furthermore, show SMARCA4 able reduce tumorigenic activity vivo while T910M, mutant form found patients,...
Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale screening studies is limited by high batch-to-batch variability, long differentiation time (10-20 weeks), production costs. This particularly relevant when brain obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, the first time, a highly standardized, reproducible, fast (5...
The transition of neural progenitors to differentiated postmitotic neurons is mainly considered irreversible in physiological conditions. In the present work, we show that Shh pathway activation through SmoM2 expression promotes dedifferentiation, re-entering cell cycle and originating medulloblastoma vivo. Notably, human adult patients inactivating mutations chromatin reader BRPF1 are associated with SMO absent pediatric adolescent patients. Here, found truncated protein, as patients, able...
Abstract Identifying causal relationships is a challenging yet crucial problem in many fields of science like epidemiology, climatology, ecology, genomics, economics and neuroscience, to mention only few. Recent studies have demonstrated that ordinal partition transition networks (OPTNs) allow inferring the coupling direction between two dynamical systems. In this work, we generalize concept study interactions among multiple systems propose new method detect causality multivariate...
Mesial temporal lobe epilepsy (MTLE) is the most common partial complex in adults and unresponsive to medications. Electrical deep brain stimulation (DBS) of hippocampus has proved effective controlling seizures epileptic rodents drug-refractory MTLE patients. However, current DBS paradigms implement arbitrary fixed-frequency or patterned stimuli, disregarding profile electrical activity. The latter, herein included hippocampal spontaneous firing, been shown follow lognormal dynamics. Here,...
Abstract Three-dimensional (3D) neural cell cultures inherently lend themselves to high-throughput network electrophysiology studies addressing brain function in health and disease a more realistic architectural complexity than two-dimensional networks. Epilepsy is the emblem of disorders, as it reflects aberrant circuit reorganization hyper-synchronization, resulting sudden uncontrolled electrical discharges (seizures). Modeling features epilepsy has so far relied on pharmacological, ionic...
Abstract Objective The compromise of the hippocampal loop is a hallmark mesial temporal lobe epilepsy (MTLE), most frequent epileptic syndrome in adult population and often refractory to medical therapy. Hippocampal sclerosis found >50% drug-refractory MTLE patients primarily involves CA1, consequently disrupting output entorhinal cortex (EC). Closed-loop deep brain stimulation (DBS) latest frontier improve MTLE; however, current approaches do not restore functional connectivity loop,...
ABSTRACT Hippocampal dysfunction is the hallmark of mesial temporal lobe epilepsy (MTLE), most common epileptic syndrome in adults and often refractory to medical therapy. Deep brain stimulation (DBS) may ameliorate drug-refractory MTLE, but it still cannot guarantee a seizure-free life. One major drawback that policy informed by trial-and-error rather than operating mode brain. Thus, optimizing DBS parameters an unmet clinical need. Here, we propose deployment hippocampal interictal...
Objective. The compromise of the hippocampal loop is a hallmark mesial temporal lobe epilepsy (MTLE), most frequent epileptic syndrome in adult population and often refractory to medical therapy. Hippocampal sclerosis found >50% drug-refractory MTLE patients primarily involves CA1, consequently disrupting output entorhinal cortex (EC). Closed-loop deep brain stimulation latest frontier improve MTLE; however, current approaches do not restore functional connectivity loop, they are designed by...
Identifying causal relationships is a challenging yet crucial problem in many fields of science like epidemiology, climatology, ecology, genomics, economics and neuroscience, to mention only few. Recent studies have demonstrated that ordinal partition transition networks (OPTNs) allow inferring the coupling direction between two dynamical systems. In this work, we generalize concept study interactions among multiple systems propose new method detect causality multivariate observational data....
This paper presents a biohybrid arrangement made of commercial microelectrode array (MEA) system for seizure-like activity detection in brain slices. The set-up takes advantage an embedded fixed-point digital signal processor (DSP) to implement neuron model and field-potential spike converter (FP2SP). is biologically plausible capable generating various firing modalities. Based on three-step algorithm, FP2SP extracts spikes from the epileptiform generated by seizure detector developed...
Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which promise to become relevant tools for disease modelling and drug screening. High-throughput screening assess a large amount of compounds using reliable fast procedures. Organoids obtained from human induced pluripotent stem cells have high batch-to-batch variability, long differentiation time (10-20 weeks), cost required production, thus limiting their use standardized large-scale studies. Here, we...