A5037486927- Pesticide Exposure and Toxicity
- Invertebrate Immune Response Mechanisms
- Insect and Pesticide Research
- Environmental Toxicology and Ecotoxicology
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Animal Ecology and Behavior Studies
- Viral Infections and Vectors
- Poisoning and overdose treatments
- Pharmacological Effects and Toxicity Studies
- Cholinesterase and Neurodegenerative Diseases
- Immune responses and vaccinations
- Air Quality and Health Impacts
- Nanoparticles: synthesis and applications
- Nanoplatforms for cancer theranostics
- Plant-based Medicinal Research
- Micro and Nano Robotics
- Peroxisome Proliferator-Activated Receptors
University of South Bohemia in České Budějovice
2016-2025
Institute of Entomology
2019-2024
Czech Academy of Sciences, Biology Centre
2019-2024
Czech Academy of Sciences
2019-2021
Sewanee: The University of the South
2020
Nemocnice České Budějovice
2019
Military Medical Academy
2004-2005
Jan Evangelista Purkyně Military Medical Academy
2004
Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature this pro-inflammatory response in mammals is complex remodeling cellular metabolism towards aerobic glycolysis. Although function bactericidal macrophages highly conserved, metabolic insect remains poorly understood. Here, we used adults fruit fly Drosophila melanogaster investigate changes that occur during acute resolution phases Streptococcus-induced...
Macrophages represent the most functionally versatile cells in animal body. In addition to recognizing and destroying pathogens, macrophages remove senescent exhausted cells, promote wound healing, govern tissue metabolic homeostasis. addition, many specialized populations of tissue-resident exhibit highly functions essential for function specific organs. Sometimes, however, cease perform their protective seemingly incomprehensible response certain stimuli leads pathology. this study, we...
The immune response is an energy-demanding process that must be coordinated with systemic metabolic changes redirecting nutrients from stores to the system. Although this interplay fundamental for function of system, underlying mechanisms remain elusive. Our data show pro-inflammatory polarization Drosophila macrophages coupled production insulin antagonist ImpL2 through activity transcription factor HIF1α. production, reflecting nutritional demands activated macrophages, subsequently...
Humanin and its analogues have been shown to protect cells against death induced by various Alzheimer's disease genes amyloid-beta-peptides in vitro: the analogue [Gly14]-humanin has also be potent reversing learning memory impairment scopolamine mice vivo. It is important validate these results using other behavioral methods. In this study, effect of des-Leu-PAGA, another (0.2 micromol kg(-1), i.p.) on 3-quinuclidinyl benzilate-induced (2 mg spatial multiple T-maze rats evaluated. Both...
In the body of multicellular organisms, macrophages play an indispensable role in maintaining tissue homeostasis by removing old, apoptotic and damaged cells. addition, allow significant remodeling plans during embryonic morphogenesis, regeneration metamorphosis. Although huge amount organic matter that must be removed these processes represents a potential source nutrients, their further use organism has not yet been addressed. Here, we document that, metamorphosis, Drosophila larval...
Using killed microorganisms or their parts to stimulate immunity for cancer treatment dates back the end of 19th century. Since then, it undergone considerable development. Our novel approach binds ligands tumor cell surface, which stimulates phagocytosis. The therapeutic effect is further amplified by simultaneous application agonists Toll-like receptors. We searched that induce both a strong and are safe humans. B16-F10 murine melanoma model was used. For stimulation phagocytosis, mannan...
Soman belongs to the most dangerous nerve agents because of low effectiveness presently available antidotes. acts by inhibiting acetylcholinesterase (AChE) both peripherally and centrally, with a subsequent accumulation neuromediator acetylcholine other metabolic changes. From data published in literature it can be concluded that exposure leading acute effects or chronic may lead delayed persistent adverse effects. The aim this study was demonstrate changes AChE butyrylcholinesterase (BuChE)...
Glucan particles derived from yeast have been recently proposed as potential drug delivery carriers. Here, we demonstrate the of glucan for protein in vivo, using insect Drosophila melanogaster a model organism. By employing genetic tools, capacity to spread efficiently through body, enter macrophages and deliver an active transcription factor successfully. Moreover, were nontoxic induced only minimal immune response. The injection did not impair ability fight survive infection by pathogenic...
Long-term effects of low doses highly toxic organophosphorus agent sarin on various hematological and biochemical markers physiological functions were studied in rats exposed to by inhalation. The results indicate that low-level sarin-exposed show long-term increase stress decrease synthesis DNA de novo without the disturbance cholinergic nervous system. Moreover, at is able induce some neurotoxic including an excitability central system 3 mo following inhalation exposure. Relatively spatial...
The present study was performed to assess and compare the therapeutic efficacy of various oximes (methoxime, BI-6, HI-6) combined with benactyzine (BNZ) in cyclosarin (GF-agent)-poisoned mice evaluate influence pretreatment PANPAL (pyridostigmine, trihexyphenidyl) on effect antidotal treatment poisoned GF-agent. In first part our experiment, methoxime, BI-6 or HI-6 combination were used for GF-poisoned mice. second experiment animals pretreated 60 min before GF-agent challenge then oxime...
Organophosphorus compounds ascribed to as nerve agents (sarin, soman, tabun, cyclosarin) are highly toxic, and considered be the most dangerous chemical compounds. All apparently share a common mechanism of cholinesterase inhibition can cause similar svmotoms. The . standard therapy, in case organophosphorus poisoning, has prophylactic use reversibly acting AChE inhibitors antidotal administration reactivators-oximes. Unfortunately, none these oximes regarded broad spectrum antidote, ie,...
1. To study the influence of pharmacological pretreatment (PANPAL or pyridostigmine combined with biperiden) and antidotal treatment (the oxime HI-6 plus atropine) on soman-induced neurotoxicity, male albino rats were poisoned a lethal dose soman (54 (g/kg i.m.; 100% LD50 value) observed at 24 hours 7 days following challenge. The neurotoxicity was evaluated using Functional observational battery an automatic measurement motor activity. 2. Pharmacological as well able to eliminate some...
1. To study the influence of antidotes on low-level sarin-induced impairment cognitive functions, rats were exposed to three various low concentrations sarin (LEVEL 1-3) for 60 minutes in inhalation chamber. In addition, one group was LEVEL 2 repeatedly. 2. Testing functions carried out using Y-maze evaluating learning and spatial memory. The correct averse behavior sarin-exposed tested several times within four weeks following exposure antidotal treatment look any impairments. 3. results...
Abstract In response to invading pathogens, macrophages metabolically polarize towards Hif1α-induced aerobic glycolysis, requiring increased supply of nutrients. Here, we show that in order obtain sufficient resources, Drosophila release the insulin/IGF antagonist ImpL2, whose expression is regulated by Hif1α. ImpL2 remotely induces lipids and carbohydrates from adipose tissue reducing insulin signaling, followed nutrient accumulation activated immune cells. thus translates metabolic...
1. To study the influence of antidotes on low-level sarin-induced alteration cognitive functions, male albino Wistar rats were exposed to three various low concentrations sarin for 60 minutes in inhalation chamber. One minute following exposure, i.m. treated with oxime HI-6 combination atropine. Control as experimental but pure air instead sarin. Cognitive functions tested using a T-maze where spatial memory and orientation evaluated. The performance sarin-exposed was several times within...
1. To study the influence of antidotes on tabun-induced neurotoxicity, rats were injected intramuscularly with organophosphate tabun (LD50). The efficacy choice antidotal treatment consisting acetylcholinesterase reactivator obidoxime and one four anticholinergic drugs (atropine, benactyzine, biperiden, scopolamine) was compared. 2. Testing neurotoxicity progress carried out using method Functional observational battery. experimental animals as well controls observed at 24 hours 7 days...
Abstract Adjustment of the cellular metabolism pro‐inflammatory macrophages is essential for their bactericidal function; however, it underlies development many human diseases if induced chronically. Therefore, intervention macrophage metabolic polarisation has been recognised as a potent strategy treatment. Although small‐molecule inhibitors affecting have identified, in vivo administration requires tool macrophage‐specific delivery to limit potential side effects. Here, we establish...