A5084968437- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Epilepsy research and treatment
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- Flavonoids in Medical Research
- Neurological Disease Mechanisms and Treatments
- Meat and Animal Product Quality
- Phosphodiesterase function and regulation
- Neurogenesis and neuroplasticity mechanisms
- Problem and Project Based Learning
- Congenital heart defects research
- Pharmacogenetics and Drug Metabolism
- Nitric Oxide and Endothelin Effects
- Renal and related cancers
- Neurological disorders and treatments
- Ion Transport and Channel Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Microbial Inactivation Methods
- Lipid Membrane Structure and Behavior
- Natural product bioactivities and synthesis
- Online and Blended Learning
- Ideological and Political Education
Niigata University
1991-2025
China Medical University
2007-2016
Shenyang Pharmaceutical University
2007-2014
China Pharmaceutical University
2008
The plasma membrane Ca2+ pump ATPase from porcine aorta was isolated by the calmodulin affinity chromatographic method of Kosk-Kosicka et al. (Kosk-Kosicka, D., Scaillet, S., and Inesi, G. (1986) J. Biol. Chem. 261, 3333-3338). Its activity restored adding either phosphatidylcholine or phosphatidylserine. Cyclic GMP-dependent protein kinase (G-kinase) stimulated enzyme in a concentration-dependent manner. However, phosphatidylinositol (PI-kinase) not detected preparation, presence necessary...
The 240-kDa, cGMP-dependent protein kinase substrate obtained from porcine aortic smooth muscle, whose phosphorylation was closely associated with stimulation of plasma membrane Ca(2+)-pump ATPase (Yoshida, Y., Sun, H.-T., Cai, J.-Q., and Imai, S. (1991) J. Biol. Chem. 266, 19819-19825), purified to near homogeneity by three successive chromatographic runs calmodulin-, concanavalin A-, heparin-Sepharose columns microsomes solubilized Triton X-100. found bind inositol 1,4,5-trisphosphate...
High-pressure treatment was utilized in this study to produce high-quality, reduced-sodium pork gels with desirable texture and sensory properties, addressing the challenge of maintaining quality low-sodium meat products meet health-conscious consumer demands. applied within range 150–200 MPa significantly reduced cooking loss while moisture content provided an ideal network structure for gels. at up 100–200 MPa, combination added sodium chloride polyphosphate, evaluated its effects on gel...
1. The aim of this study was to investigate the inhibitory effect morusin on Glucuronosyltransferase (UGT) isoforms and cytochrome P450 enzymes (CYP450s). We also investigated metabolism in human, rat, dog, monkey, minipig liver microsomes.2. 100 μM exhibited strong inhibition all UGTs CYP450s. half concentration (IC50) values for CYP3A4, CYP1A2, CYP2C9, CYP2E1, UGT1A6, UGT1A7, UGT1A8 were 2.13, 1.27, 3.18, 9.28, 4.23, 0.98, 3.00 μM, kinetic parameters (Ki) 1.34, 1.16, 2.98, 6.23, 4.09,...
Culture of hippocampal neurons in low-Mg 2+ medium (low-Mg neurons) results induction continuous seizure activity. However, the underlying mechanism contribution low Mg to hyperexcitability has not been clarified. Our data, obtained using patch-clamp technique, show that voltage-gated Na + channel (VGSC) activity, which is associated with a persistent, noninactivating current ( I Na,P ), was modulated by calmodulin (CaM) concentration-dependent manner normal and neurons, but activity more...
A plasma membrane Ca2+-pump ATPase preparation purified from porcine aorta was incubated with cGMP-dependent protein kinase (G-kinase) under the conditions which dose-dependent stimulation of enzyme by G-kinase observed. Several proteins were phosphorylated, but two isoforms molecular masses 135- and 145-kDa not phosphorylated. The that phosphorylated identified in our previous study as 135-kDa isoform ATPase, on basis its almost identical mobility SDS-PAGE, found to be another a mass 138...
Cytochrome p450 (CYP450) enzymes are predominantly involved in Phase I metabolism of xenobiotics. In this study, the CYP450 isoforms xanthotoxol were identified using recombinant CYP450s. addition, inhibitory effects on eight and its pharmacokinetic parameters determined human liver microsomes. CYP1A2, one CYP450s, played a key role compared to other Xanthotoxol showed stronger inhibition CYP3A4 CYP1A2 isoenzymes with IC 50 7.43 μ M for 27.82 CYP1A2. The values kinetic (Ki) 21.15 2.22...
Voltage-dependent calcium channels (VDCCs) are key elements in epileptogenesis. There several binding-sites linked to calmodulin (CaM) and potential CaM-dependent protein kinase II (CaMKII)-mediated phosphorylation sites CaV1.2. The tremor rat model (TRM) exhibits absence‑like seizures from 8 weeks of age. present study was performed detect changes the Ca2+/CaV1.2/CaM/CaMKII pathway TRMs cultured hippocampal neurons exposed Mg2+‑free solution. expression levels CaV1.2, CaM phosphorylated...
With the successful use of problem-based learning(PBL)in medical education,it was an attempt to apply Intemet and PBL in pharmacological teaching.As a result,it found that this method could stimulate student's interest study.promote students'ability self-directed learning,foster their critical thinking skills,but also save discussion time clagsroom while providing deeper make satisfactory teaching effect. Key words: Internet; Problem-based learning; Pharmacology