A5100363254- Tuberculosis Research and Epidemiology
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Phenothiazines and Benzothiazines Synthesis and Activities
- Cancer-related gene regulation
- Bioactive natural compounds
- Antibiotics Pharmacokinetics and Efficacy
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Viral Infections and Vectors
- Biological and pharmacological studies of plants
- HIV Research and Treatment
- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- ATP Synthase and ATPases Research
- Vector-Borne Animal Diseases
- Microbial Natural Products and Biosynthesis
- Ubiquitin and proteasome pathways
- Molecular Sensors and Ion Detection
- Silymarin and Mushroom Poisoning
- Protein Degradation and Inhibitors
- Neuroblastoma Research and Treatments
- Healthcare and Venom Research
- Analytical Chemistry and Chromatography
- Sirtuins and Resveratrol in Medicine
Ningxia University
2021-2025
Institute of Microbiology
2018-2025
Chinese Academy of Sciences
2018-2025
Guizhou University
2024
National Center for Nanoscience and Technology
2024
Gladstone Institutes
2024
University of Chinese Academy of Sciences
2024
East China Normal University
2024
Prothena (United States)
2024
California Institute of Technology
2021-2023
Abstract Despite the success of combination antiretroviral therapy (ART) for individuals living with HIV, mild forms HIV-associated neurocognitive disorder (HAND) continue to occur. Brain microglia form principal target HIV infection in brain. It remains unknown how these cells leads neuroinflammation, neuronal dysfunction, and/or death observed HAND. Utilizing two different inducible pluripotent stem cell-derived brain organoid models (cerebral and choroid plexus [ChP] organoids) containing...
Bovine Coronavirus (BCoV) is a significant pathogen responsible for neonatal calf diarrhea, winter dysentery in adult cattle, and bovine respiratory diseases. Infection with the virus can result hemorrhagic decreased milk production, potentially fatal outcomes leading to considerable economic repercussions cattle industry. Efficient management of BCoV relies on swift precise detection techniques. CHO cells were utilized express secreted recombinant nucleocapsid protein (N), whereby rabbit...
Clofazimine (CFZ), a member of the riminophenazine class, has been studied in clinical trials for treatment multidrug-resistant tuberculosis (MDR-TB). CFZ several side effects which can be attributed to its extremely high lipophilicity. A series novel analogues bearing C-2 pyridyl substituent was designed and synthesized with goal maintaining potent activity against Mycobacterium (M. tuberculosis) while improving upon safety profile by lowering All compounds were evaluated their vitro...
A novel clicked bisubstrate analogue (NAM-TZ-SPKRIA) was designed and synthesized to be a potent, selective, first NTMT1 inhibitor.
Blood-sucking ticks are obligate parasites and vectors of a variety human animal viruses. Some tick-borne viruses have been identified as pathogens infectious diseases in humans or animals, potentially imposing significant public health burdens threats to the husbandry industry. Therefore, identifying profiles will provide valuable information about evolution pathogen ecology In this study, we investigated viromes parasitic collected from body surfaces herbivores Xinjiang Uyghur Autonomous...
The objective of this study was to develop an indirect ELISA utilizing a polyclonal antibody against bovine rotavirus (BRV) VP6 protein. To achieve this, pcDNA3.1-VP6, recombinant eukaryotic expression plasmid, constructed based on the sequence conserved BRV gene and transfected into CHO-K1 cells using transient transfection method. protein purified as coating antigen nickel ion affinity chromatography, subsequently established. found that optimal concentration for 1 μg/mL. blocking solution...
The protein N-terminal methyltransferase 1 (NTMT1) catalyzes the transfer of methyl group from S-adenosyl-l-methionine to α-amine, resulting in formation S-adenosyl-l-homocysteine and α-N-methylated proteins. NTMT1 is an interesting potential anticancer target because it overexpressed gastrointestinal cancers plays important role cell mitosis. To gain insight into biochemical mechanism NTMT1, we have characterized kinetic recombinant using a fluorescence assay mass spectrometry. results...
Abstract The exposure of healthy subjects to high altitude represents a model explore the pathophysiology diseases related tissue hypoxia. We explored plasma metabolomics approach detect alterations induced by altitude. Plasma samples were collected from 60 both on plain and at (5300 m). Metabolite profiling was performed gas chromatography-mass spectrometry (GC-MS) ultra-performance liquid chromatography-quadrupole time-of-flight mass (UPLC-QTOFMS) in conjunction with univariate...
8-Nitrobenzothiazinones (BTZs) typified by the second-generation analogue PBTZ169 are a new class of antitubercular agents. The activity BTZs and lipophilicity tightly coupled since molecular target DprE1 is located in mycobacterial cell envelope. A series analogues was designed to address notorious insolubility while preserving required lipophilicity. This accomplished decreasing planarity symmetry through bioisosteric replacement piperazine moiety with spirocyclic bicyclic diamines....
The aim of this study was to investigate the effect amorphous solid dispersions (ASDs) on dissolution rate and oral bioavailability Quercetin (Que). First, we prepared Que ASDs with various excipients using hot-melt extrusion find best option. X-ray diffraction (XRD), infrared spectroscopy (IR), Raman were used examine formation Que. Wetting process studied by contact angle solution process. abilities HPMC inhibit crystallization improve membrane permeability demonstrated fluorescence...
Abstract Type 2 diabetic kidney disease (T2DKD) is a common microvascular complication of type diabetes mellitus (T2DM), and its incidence significantly increasing. Microinflammation plays an important role in the development T2DKD. Based on this, this study investigated value inflammatory markers including neutrophil–lymphocyte ratio (NLR), high-sensitivity C-reactive protein (hs-CRP), monocyte chemoattractant protein-1 (MCP-1) prediction This was cross-sectional survey study. A total 90...
Stilbene synthase (STS), is a pivotal gene involved in the biosynthesis and regulation of stilbenes compounds, playing crucial role plant growth, development stress adaptation. Meantime, stilbene compounds hold promising prospects wide-ranging clinical applications. Rheum officinale contains abundant yet precise regulatory genes governing their remain elusive. In this study, ORF RoSTS was amplified, yielding length 1,140 bp encoding protein consisting 379 amino acids with relative molecular...
The aim of this study was to establish and evaluate a simultaneous amplification testing method for detection the Mycobacterium tuberculosis complex (SAT-TB assay) in clinical specimens by using isothermal RNA real-time fluorescence detection. In SAT-TB assay, 170-bp M. 16S rRNA fragment is reverse transcribed DNA use Moloney murine leukemia virus (M-MLV) transcriptase, specific primers incorporating T7 promoter sequence, undergoes successive cycles polymerase. Using PCR instrument,...
Ebola virus is the causative agent of disease in humans. The lethality infection about 50%, supporting urgent need to develop anti-Ebola drugs. Glycoprotein (GP) only surface protein virus, which functionally critical for attach and enter host cells, a promising target drug development. In this study, using recombinant HIV-1/Ebola pseudovirus platform we previously established, evaluated small molecule library containing various quinoline compounds entry inhibitors. Some specifically...
Decaprenylphosphoryl-β-d-ribose oxidase (DprE1) is the flavoprotein subunit of decaprenylphosphoryl-d-ribose epimerase involved in cell wall synthesis Mycobacterium tuberculosis and catalyzes conversion decaprenylphosphoryl ribose to arabinose. DprE1 a potential target against tuberculosis, including multidrug-resistant tuberculosis. We identified inhibitors from ChemDiv dataset through virtual screening based on pharmacophore molecular docking. Thirty selected compounds were subjected...
Optochin, a cinchona alkaloid derivative discovered over 100 years ago, possesses highly selective antibacterial activity toward Streptococcus pneumoniae. Pneumococcal disease remains the leading source of bacterial pneumonia and meningitis worldwide. The structure–activity relationships optochin were examined through modification to both quinoline quinuclidine subunits, which led identification analogue 48 with substantially improved activity. Resistance molecular modeling studies indicate...
// Tangjingjun Liu 1, 7, * , Yao Gang Zhang 1 Ye Wang 2 Bin Deng Jialei Song 8 Xiaogang Li Fei Han Xiao 7 Jue Yang Lei Xia 3 You-Jun 4 Maksym Plachynta Mu Chen Yan Shuzhen Heng Luo Eldad Zacksenhaus 5, 6 Xiaojiang Hao 3, Yaacov Ben-David Department of Biology and Chemistry, The Key Laboratory Chemistry for Natural Products Guizhou Province Chinese Academy Sciences, Guizhou, China College Ecology, Lishui University, Zhejiang, School Pharmaceutical Anatomy, Norman Bethune Medicine, Jilin...
Abstract Skimmin, a natural coumarin derivate, has been showed to be protective against experimental diabetic nephropathy; however, its effect on cardiomyopathy (DCM) is not clarified. By using in vitro and vivo models, we investigated skimmin's impaired heart tissues DCM. DCM was induced by streptozotocin (STZ, 60 mg/kg) Sprague Dawley rats, rats were treated with either skimmin (15 or 30 the vehicle for 16 weeks, normal used as control. Hematoxylin eosin Masson's trichrome staining...
Abstract Milk‐derived extracellular vesicles (M‐EVs) are low‐cost, can be prepared in large quantities, and cross the gastrointestinal barrier for oral administration. However, composition of milk is complex, M‐EVs obtained by different extraction methods may affect their delivery. Based on this, a new method extracting based cryogenic freezing treatment (Cryo‐M‐EVs) proposed compared with previously reported acetic acid (Acid‐M‐EVs) conventional ultracentrifugation (Ulltr‐M‐EVs). The...