A5100355760- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Cancer therapeutics and mechanisms
- Infectious Diseases and Tuberculosis
- Viral Infections and Outbreaks Research
- Bacillus and Francisella bacterial research
- Respiratory viral infections research
- Infectious Diseases and Mycology
- Hepatitis B Virus Studies
- Kidney Stones and Urolithiasis Treatments
- Diagnosis and treatment of tuberculosis
- Selenium in Biological Systems
- Virus-based gene therapy research
- Microbial infections and disease research
- Cytokine Signaling Pathways and Interactions
- Mosquito-borne diseases and control
- Bacterial Identification and Susceptibility Testing
- Traditional Chinese Medicine Studies
- Antibiotics Pharmacokinetics and Efficacy
- Biochemical and Molecular Research
- Biosensors and Analytical Detection
- Infections and bacterial resistance
Guangzhou Medical University
2019-2024
Wuhan Institute of Virology
2018-2024
Chinese Academy of Sciences
2016-2024
University at Buffalo, State University of New York
2024
First Affiliated Hospital of Guangzhou Medical University
2019-2024
Tianjin Children's Hospital
2023
Tianjin University
2023
Guangzhou Institutes of Biomedicine and Health
2016-2022
Beijing Chest Hospital
2010-2022
Capital Medical University
2013-2022
Little is known about the biochemical environment in phagosomes harboring an infectious agent. To assess state of this organelle we captured transcriptional responses Mycobacterium tuberculosis (MTB) macrophages from wild-type and nitric oxide (NO) synthase 2–deficient mice before after immunologic activation. The intraphagosomal transcriptome was compared with MTB standard broth culture during growth diverse conditions designed to simulate features phagosomal environment. Genes expressed...
Chemotherapeutic options to treat tuberculosis are severely restricted by the intrinsic resistance of Mycobacterium majority clinically applied antibiotics. Such is partially provided low permeability their unique cell envelope. Here we describe a complementary system that coordinates drugs have penetrated envelope, allowing mycobacteria tolerate diverse classes antibiotics inhibit cytoplasmic targets. This depends on whiB7 , gene pathogenic shares with Streptomyces phylogenetically related...
ABSTRACT The multiresistance gene cfr was identified for the first time in an Enterococcus faecalis isolate of animal origin. 32,388-bp plasmid pEF-01, which carried gene, sequenced completely. Three copies insertion sequence IS 1216 were and detection a - -containing amplicon by inverse PCR suggests that may play role dissemination recombination process.
Lassa virus (LASV) belongs to the Mammarenavirus genus (family Arenaviridae) and causes severe hemorrhagic fever in humans. At present, there are no Food Drug Administration (FDA)-approved drugs or vaccines specific for LASV. Here, high-throughput screening of an FDA-approved drug library was performed against LASV entry by using pseudotype bearing envelope glycoprotein (GPC). Two hit compounds, lacidipine phenothrin, were identified as inhibitors micromolar range. A mechanistic study...
Abstract Buruli ulcer (BU) is an emerging infectious disease that causes disfiguring skin ulcers. The causative agent, Mycobacterium ulcerans , secretes toxin called mycolactone triggers inflammation and immunopathology. Existing treatments are lengthy consist of drugs developed for tuberculosis. Here, we report a pyrazolo[1,5-a]pyridine-3-carboxamide, TB47, highly bactericidal against M. both in vitro vivo. In the validated mouse model BU, TB47 alone reduces burden footpads by more than 2.5...
Epidemiological evidence of over 9000 people suggests that daily intake vinegar whose principal bioactive component is acetic acid associated with a reduced risk nephrolithiasis. The underlying mechanism, however, remains largely unknown.
Clofazimine (CFZ), a member of the riminophenazine class, has been studied in clinical trials for treatment multidrug-resistant tuberculosis (MDR-TB). CFZ several side effects which can be attributed to its extremely high lipophilicity. A series novel analogues bearing C-2 pyridyl substituent was designed and synthesized with goal maintaining potent activity against Mycobacterium (M. tuberculosis) while improving upon safety profile by lowering All compounds were evaluated their vitro...
In China, fosfomycin alone or in combination with other antibiotics is commonly used the treatment of infections caused by Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Although fosfomycin-resistant S. strains have continued to emerge and increase, research on them rare. order determine prevalence mechanisms resistance MRSA clinical isolates, a total 96 non-duplicate isolates were collected from blood cerebrospinal fluid samples at Huashan Hospital...
Abstract There remains a lack of effective drugs to alleviate the kidney stones caused by oxidative stress and inflammatory damage. The MOF‐818 nanozyme is utilized lessen generation reactive oxygen species (ROS) effectively, restore membrane potential mitochondria, regulate cell cycle, decrease death, hinder recruitment macrophages, mitigate release factors in macrophages. These effects are attributed nanozyme's ability mimic enzyme properties catalase (CAT) superoxide dismutase (SOD). It...
The mosquito-borne Japanese encephalitis virus (JEV) causes serious illness worldwide that is associated with high morbidity and mortality. Currently, there are no effective drugs approved for the treatment of JEV infection. Drug-repurposing screening an alternative approach to discover potential antiviral agents.
Clofazimine, a member of the riminophenazine class, is one few antibiotics that are still active against multidrug-resistant Mycobacterium tuberculosis (M. tuberculosis). However, clinical utility this agent limited by its undesirable physicochemical properties and skin pigmentation potential. With goal maintaining potent antituberculosis activity while improving lowering potential, series novel derivatives containing 2-methoxypyridylamino substituent at C-2 position phenazine nucleus were...
With the increasing spread of methicillin-resistant Staphylococcus aureus worldwide, fosfomycin has begun to be used more often, either alone or in combination with other antibiotics, for treating S. infections, resulting emergence fosfomycin-resistant strains. Fosfomycin resistance is reported mediated by fosfomycin-modifying enzymes (FosA, FosB, FosC, and FosX) mutations target enzyme MurA membrane transporter proteins UhpT GlpT. Our previous studies indicated that fos genes might not...
Objective: Lassa virus (LASV) glycoprotein complex (GPC) contains retained stable-signal peptide (SSP), 1 (GP1), and 2 (GP2). Through serial passaging of LASV with inhibitors, adaptive mutants were obtained, most which had mutations in the transmembrane (TM) domain GP2. Characterizing fusion inhibitor target within TM GP2 provided insights for development drugs vaccines. Methods: We conducted membrane fusion, IIH6 inhibition, thermostability, viral growth kinetics assays to characterize...
Calcium-containing stones represent the most common form of kidney calculi, frequently linked to idiopathic hypercalciuria, though their precise pathogenesis remains elusive. This research aimed elucidate molecular mechanisms involved by employing urinary exosomal microRNAs as proxies for renal tissue analysis. Elevated miR-148b-5p levels were observed in exosomes derived from patients with stones. Systemic administration rat models resulted heightened calcium excretion, whereas its...
Mycobacterium abscessus (MAB), which manifests in the pulmonary system, is one of neglected causes nontuberculous mycobacteria (NTM) infection. Treatment against MAB difficult, characterized by its intrinsic antibiotic drug resistance. Lysine acetylation can alter physiochemical property proteins living organisms. This study aimed to determine if this protein post-translational modification (PTM) exists a clinical isolate M. GZ002. We used antiacetyl-lysine immunoprecipitation enrich...
Mycobacterium abscessus is intrinsically resistant to most antimicrobial agents. The emerging infections caused by M. and the lack of effective treatment call for rapid attention. Here, we intended construct a selectable marker-free autoluminescent strain (designated UAlMab) as real-time reporter facilitate discovery drugs regimens treating UAlMab was constructed using dif/Xer recombinase system. In vitro in vivo activities several drugs, including clofazimine TB47, recently reported...
A series of pyrazolo[1,5-a]pyridine-3-carboxamide (PPA) derivatives bearing diaryl side chain was designed and synthesized as new antituberculosis agents, aiming to improve the efficacy toward drug resistant Mycobacterium tuberculosis (Mtb) strains. Most substituted diphenyl heterodiaryl PPAs exhibited excellent in vitro potency against susceptive H37Rv strain (MIC < 0.002–0.381 μg/mL) Mtb strains (INH-resistant (rINH), MIC 0.002–0.465 μg/mL; RMP-resistant (rRMP), 0.002–0.004 μg/mL)....
Abstract Zika virus (ZIKV) is an infectious disease that has become important concern worldwide, it associates with neurological disorders and congenital malformations in adults, also leading to fetal intrauterine growth restriction microcephaly during pregnancy. However, there are currently no approved vaccines or specific antiviral drugs for preventing treating ZIKV infection. Here, we show two FDA-approved Na + /K -ATPase inhibitors, ouabain digoxin, can block infection at the replication...