A5017035150- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Blood properties and coagulation
- Neonatal Health and Biochemistry
- Diabetes and associated disorders
- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Hemoglobinopathies and Related Disorders
- Blood transfusion and management
- Pancreatic function and diabetes
- Connexins and lens biology
- Heat shock proteins research
- Extracellular vesicles in disease
- Receptor Mechanisms and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Advanced Glycation End Products research
- Hemoglobin structure and function
- RNA regulation and disease
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Lymphatic Disorders and Treatments
- Mitochondrial Function and Pathology
- Calcium signaling and nucleotide metabolism
Radboud University Nijmegen
2011-2020
Radboud University Medical Center
2011-2020
Radboud Institute for Molecular Life Sciences
2003-2020
University Medical Center
2015-2019
Rijnstate Hospital
2002-2010
Sanquin
2002-2009
Johannes Kepler University of Linz
2007
Max Perutz Labs
2007
Medical University of Vienna
2007
Leiden University
2004
Previous studies demonstrated that 20% of haemoglobin is lost from circulating erythrocytes during their total lifespan by vesiculation. To study whether removal molecules other than membrane-bound were present in erythrocyte-derived vesicles, flow cytometry and immunoblot analysis employed to examine the presence phosphatidylserine (PS) IgG, senescent cell antigens respectively. It was 67% glycophorin A-positive vesicles exposed PS, half these also contained IgG. Immunoblot revealed a...
The use of fresh red blood cells (RBCs) is recommended for critically ill patients and undergoing surgery, although there no conclusive evidence that this beneficial. In follow-up study, the short-term long-term recovery irradiated, leukoreduced RBCs transfused after either a short storage (SS) or long (LS) period were compared. By consecutive transfusion with SS LS period, direct comparison their survival within same patient was possible.Ten transfusion-requiring each received RCCs (stored...
During storage of red cells (RBCs) for transfusion, RBCs undergo a number biochemical and morphologic changes. To be able to identify the mechanisms underlying these lesions, proteomic analysis membranes their vesicles was performed during various periods in blood bank conditions.RBCs were isolated from after periods. The proteins RBC separated by gel electrophoresis identified semiquantitative analysis.Our findings confirm previous data, such as storage-associated increase hemoglobin...
BACKGROUND: The release of vesicles by red blood cells (RBCs) occurs in vivo and vitro under various conditions. Vesiculation also takes place during RBC storage results the accumulation units. membrane protein composition storage‐associated has not been studied detail. characterization vesicular might hint at underlying mechanism changes general vesiculation process particular. STUDY DESIGN AND METHODS: Vesicles from RBCs that had stored for periods were isolated same units used to generate...
BACKGROUND: During storage of red cells (RBCs) for transfusion, RBCs undergo a number biochemical and morphologic changes. To be able to identify the mechanisms underlying these lesions, proteomic analysis membranes their vesicles was performed during various periods in blood bank conditions. STUDY DESIGN AND METHODS: were isolated from after periods. The proteins RBC separated by gel electrophoresis identified semiquantitative analysis. RESULTS: Our findings confirm previous data, such as...
Summary Mature, circulating erythrocytes undergo senescence, which limits their life span to approximately 120 d. Upon injury, may suicidal erythrocyte death or eryptosis, accelerate senescence and shorten survival. Eryptosis is defined as cell shrinkage exposure of phosphatidylserine at the surface. Triggers eryptosis include oxidative stress. The present study addresses impact age on relative susceptibility eryptosis. Erythrocytes were separated into five fractions, based age‐associated...
COGNITIVE impairment and dementia are common in the later stages of Parkinson's disease (PD). Neuropathological examination demented PD (PDD) patients often reveals changes that typical Alzheimer's (AD). In AD, there is a massive reactive gliosis increased expression small heat shock proteins (hsp) hsp27 αB-crystallin. Since these characteristic for astrocytes we investigated their brains PDD patients. The results were compared with those obtained non-demented We found (1) no detectable hsp...
Background/Aims: Erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and phosphatidylserine exposure at the erythrocyte outer membrane. Susceptibility to eryptosis is enhanced in aged erythrocytes stimulated NFκB-inhibitors Bay 11-7082 parthenolide. Here we explored whether expression of NFκB susceptibility inhibitor-induced sensitive age. Methods: Human were separated into five fractions, based on age-associated characteristics density volume. compared ß-actin...
Inflammation enhances the secretion of sphingomyelinases (SMases). SMases catalyze hydrolysis sphingomyelin into phosphocholine and ceramide. In erythrocytes, ceramide formation leads to exposure removal signal phosphatidylserine (PS), creating a potential link between SMase activity anemia inflammation. Therefore, we studied effects on various pathophysiologically relevant parameters erythrocyte homeostasis. Time-lapse confocal microscopy revealed SMase-induced transition from discoid...
Red blood cells (RBCs) undergo extensive deformation when travelling through the microcapillaries. Deformability, combined result of properties membrane-cytoskeleton complex, surface area-to-volume ratio, and hemoglobin content, is a critical determinant capillary flow. During bank storage in many pathophysiological conditions, RBC morphology changes, which has been suggested to be associated with decreased deformability removal RBC. While various techniques provide information on...