A5088550475- Catalysis and Oxidation Reactions
- Protein purification and stability
- Enzyme Structure and Function
- Catalytic Processes in Materials Science
- Catalysts for Methane Reforming
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Conducting polymers and applications
- Organic Electronics and Photovoltaics
- Viral gastroenteritis research and epidemiology
- Metal-Organic Frameworks: Synthesis and Applications
- Glycosylation and Glycoproteins Research
- Perovskite Materials and Applications
- Advanced NMR Techniques and Applications
- Biosimilars and Bioanalytical Methods
- Protein Kinase Regulation and GTPase Signaling
- NMR spectroscopy and applications
- Chemical Synthesis and Characterization
- Spectroscopy and Quantum Chemical Studies
- Advanced Drug Delivery Systems
- Nanopore and Nanochannel Transport Studies
- Organic Light-Emitting Diodes Research
- Covalent Organic Framework Applications
- Bacteriophages and microbial interactions
- Enzyme function and inhibition
Nanjing Tech University
2022-2023
Pfizer (United States)
2010-2023
South China University of Technology
2017-2022
The Synergetic Innovation Center for Advanced Materials
2022
Kunming University of Science and Technology
2019-2021
Ningbo University
2011
Wuhan Institute of Physics and Mathematics
2010
Chinese Academy of Sciences
2009-2010
Indiana University School of Medicine
2007-2010
Indiana University – Purdue University Indianapolis
2007-2010
Trimethylamine n-oxide (TMAO) is a naturally occurring osmolyte that stabilizes proteins and offsets the destabilizing effects of urea. To investigate molecular mechanism these effects, we have studied thermodynamics interaction between TMAO protein functional groups. The solubilities homologous series cyclic dipeptides were measured by differential refractive index dissolution heats determined calorimetrically as function concentration at 25 °C. transfer free energy amide unit (−CONH−) from...
The active sites of a mixed Cu/Ce material and the doping effect typical element (iron, Fe) on species catalytic behavior for CO preferential oxidation in rich H2 (CO-PROX) were investigated by situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), oxygen storage capacity measurement (OSC) combined with designed temperature-programmed reduction (TPR), along Raman, X-ray photoelectron (XPS), diffraction (XRD), desorption/reduction (CO-TPD/TPR). These results showed that...
The effects of urea on protein stability have been studied using a model system in which we determined the energetics dissolution homologous series cyclic dipeptides into aqueous solutions varying concentration at 25 degrees C calorimetry. data support denatures proteins by decreasing hydrophobic effect and directly binding to amide units via hydrogen bonds. indicate also that enthalpy bond formation water is considerably higher than previously estimated. Previous estimates included...
The functional specificity of type 1 protein phosphatases (PP1) depends on the associated regulatory/targeting and inhibitory subunits. To gain insights into mechanism PP1 regulation by inhibitor-2, an ancient intrinsically disordered regulator, we solved crystal structure complex to 2.5A resolution. Our studies show that, when complexed with PP1c, I-2 acquires three regions order: site 1, residues 12-17, binds adjacent a region recognized many regulators; 2, amino acids 44-56, interacts...
Lipid nanoparticles (LNPs) have been used as a carrier for messenger RNA (mRNA) vaccines. Surface properties of LNPs are important to the stability and function mRNA Polyethylene-glycol (PEG) is functional lipid at surface that improves colloidal stability, increases circulation time, impacts cellular uptake. In this study, we explore in-depth composition mRNA-LNPs using high-field nuclear magnetic resonance (NMR) spectroscopy. Our results provide unique profile intact identifying PEG chains...
Abstract Compared with inverted 3D perovskite solar cell (PSCs), quasi‐2D PSCs have advantages in device stability, but the efficiency is still lagging behind. Constructing polymer hole‐transporting materials (HTMs) passivation functions to improve buried interface and crystallization properties of films one effective strategies performance PSCs. Herein, two novel side‐chain functionalized HTMs containing methylthio‐based groups are designed, named PVCz‐SMeTPA PVCz‐SMeDAD, for Benefited from...
Open AccessCCS ChemistryRESEARCH ARTICLE7 Nov 2022Unique Ligand Exchange Dynamics of Metal–Organic Polyhedra for Vitrimer-like Gas Separation Membranes Mingxin Zhang†, Haitao Yu†, Qin Zou, Zi-Ang Li, Yuyan Lai, Linkun Cai and Panchao Yin Zhang† State Key Laboratory Luminescent Materials Devices, South China Advanced Institute Soft Matter Science Technology, University Guangzhou 510640 †M. Zhang H. Yu contributed equally to this work.Google Scholar More articles by author , Yu† Zou Google Li...
The growing importance of biologics and biosimilars as therapeutic diagnostic agents is giving rise to new demands for analytical methodology that can quickly accurately assess the chemical physical state protein-based products. A particular challenge exists in characterization where proper fold extent disorder a protein major concern. ability NMR reflect structural dynamic properties proteins well recognized, but sensitivity limitations high levels interference from excipients typical...
Aggregation and self-association in protein-based biotherapeutics are critical quality attributes that tightly controlled by the manufacturing process. Aggregates have potential to elicit immune reactions, including neutralizing anti-drug antibodies, which can diminish drug's efficacy upon subsequent dosing. The structural basis of reversible self-association, a form non-covalent aggregation native state, is only beginning emerge for many biologics often unique given molecule. In present...
With access to the solution structures of nanocomposites coordination nanocages (CNCs) via scattering and chromatography techniques, their mysterious dynamics have been, for first time, resolved, interestingly, surface macromolecules can be substituted by extra free in solutions. Obvious exchange observed mixtures CNC at high temperatures, revising understanding nanocomposites. Being distinct from a simple complex, quantified indicates typical logarithmic time dependence with dissociation as...
Abstract Knowledge of three-dimensional (3D) structures each individual particles asymmetric and flexible proteins is essential in understanding those proteins’ functions; but their are difficult to determine. Electron tomography (ET) provides a tool for imaging single unique biological object from series tilted angles, it challenging image protein reconstruction due the imperfect mechanical control capability specimen goniometer under both medium high magnification (approximately...
In mice, the major urinary proteins (MUP) play a key role in pheromonal communication by binding and transporting semiochemicals. MUP-IV is only isoform known to be expressed vomeronasal mucosa. comparison with MUP isoforms that are abundantly excreted urine, highly specific for male mouse pheromone 2-sec-butyl-4,5-dihydrothiazole (SBT). To examine structural basis of this ligand preference, we determined X-ray crystal structure bound three pheromones: SBT, 2,5-dimethylpyrazine, 2-heptanone....
Conjugate vaccines prepared with the cross-reactive material 197 (CRM197) carrier protein have been successful in clinic and are of great interest field immunotherapy. One route to preparing peptide–CRM197 conjugate involves an activation–conjugation strategy, effectively coupling lysine residues on cysteine thiolate groups peptide using a heterobifunctional linker as activation agent. This method has found result two distinct populations conjugates, believed be conformational change CRM197...
The structures and thermal properties of the multiple ordered phases in ethylene−octene ethylene−butene copolymers have been studied using a combination solid-state NMR DSC. Three types phases, namely orthorhombic, monoclinic, rotator (or mobile phase), found to coexist these two ethylene copolymers. Our experimental results demonstrate that slow-spinning CP/MAS 13C provides convenient method discern signals three different measure chemical shift tensors orthorhombic monoclinic phases....
We observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs whose primary difference was genome length. To determine a possible reason for this outcome, we characterized the proportion composition of empty packaged capsids. Both analytical ultracentrifugation (AUC) centrifugation sedimentation (DCS, rapid quantitative method measuring adenoviral packaging variants) were employed an initial assessment showed multiple species abundance...
The effects of urea on protein stability have been studied using a model system in which we determined the energetics dissolution homologous series cyclic dipeptides into aqueous solutions varying concentration at 25°C calorimetry. data support denatures proteins by decreasing hydrophobic effect and directly binding to amide units via hydrogen bonds. indicate also that enthalpy bond formation water is considerably higher than previously estimated. Previous estimates included contribution...