A5003849187- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Extracellular vesicles in disease
- Field-Flow Fractionation Techniques
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Minerals Flotation and Separation Techniques
- Electrostatics and Colloid Interactions
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Membrane-based Ion Separation Techniques
- Reproductive Biology and Fertility
- Innovative Microfluidic and Catalytic Techniques Innovation
- Machine Learning and ELM
- Sperm and Testicular Function
- Energy Harvesting in Wireless Networks
- Mineral Processing and Grinding
- Electrohydrodynamics and Fluid Dynamics
- Thermal properties of materials
Mayo Clinic
2023
University of Utah
2014-2022
The influence of buffer substitution and dilution effects on exosome size electrophoretic mobility were shown for the first time. Cyclical electrical field flow fractionation (Cy-El-FFF) in various substituted fluids was applied to exosomes other particles. Tested carrier deionized (DI) water, 1× phosphate buffered saline (PBS), 0.308 M trehalose, 2% isopropyl alcohol (IPA) influenced Cy-El-FFF-mediated isolation A375 melanoma exosomes. All fractograms revealed a crescent-shaped trend...
Nanoscale and microscale cell-derived extracellular vesicle types subtypes are of significant interest to researchers in biology medicine. Extracellular vesicles (EVs) have diagnostic therapeutic potential terms biomarker nanomedicine applications. To enable such applications, EVs must be isolated from biological fluids or separated other EV types. Developing methods fractionate is great importance researchers. Our goal was begin develop a device that would separate medium (mEVs,...
Although many properties for small extracellular vesicles (sEVs, formerly termed "exosomes") isolated at ∼100 000g are known, a wide range of values reported their electrophoretic mobility (EM) measurements. This paper reports the first time effect dilution on EM U87 glioblastoma cell-derived and plasma-derived sEVs medium size EVs (mEVs, commonly "oncosomes") preisolated by differential centrifugation. Furthermore, resalting mEVs was evaluated. The showed an increase as salt concentration...
Abstract Sperm preparation is critical to achieving a successful intrauterine insemination and requires the processing of semen sample remove white blood cells, wash away seminal plasma, reduce volume. We present an automated instrument capable performing sperm starting with diluted sample. compare our device against density gradient centrifugation by 0.5 mL portions patient samples through each treatment. In 5 min operating time, recovers average 86% all 82% progressively motile from...
Electrical field flow fractionation (EFFF) is a particle separation method that employs an electrical to cause particles in the carrier transferring vertical direction. The characteristics of EFFF are one important aspects explore how work on channel. equivalent circuit model channel was built. experiments chronoamperometry and electrochemical impedance spectroscopy (EIS) were conducted utilised fit data EIS fitting compared with experimental EIS. result indicates highly quantified data. It...
In this work, a new high-volume, continuous particle separation device that separates based upon size and charge is described. Two flow-electrical-split-flow lateral transport thin (Fl-El-SPLITT) architectures (a platinum electrode on porous membrane graphite under membrane) were developed shown to improve separations over purely electrical-SPLITT device. The FL-El-SPLITT architecture achieved the best of approximately 60% small (28 nm) vs large (1000 polystyrene particles. Fl-El-SPLITT...