Transparent surfaces within microfluidic devices are essential for accurate quantification of chemical, biological, and mechanical interactions. Here, we report how to create low-cost, rapid 3D-printed that optically free from artifacts have transparent suitable visualizing a variety fluid phenomenon. The methodology described here can be used creating high-pressure systems (significantly higher than PDMS–glass bonding). We develop methods annealing Poly-Lactic Acid (PLA) demonstrating heat...

Microfluidic technology has potential to separate sperm cells from unwanted debris while improving the effectiveness of assisted reproductive technologies (ART). Current clinical protocol limitations regarding separation other cells/cellular can lead low recovery when sample contains a concentration mostly motility and high debris, such as in semen samples patients with pyospermia [high white blood cell (WBC) semen]. This study demonstrates label-free using inertial microfluidics. The...

Inertial microfluidics are used to continuously separate non-motile sperm from blood and other contaminants.

A microfluidic PCR device was developed that enables DNA amplification at speeds as fast 2 s/cycle, with concurrent detection and amplification. Two targets were amplified from human genomic DNA. By observing the fluorescence emitted by a dye while sample is amplified, it possible to obtain both qPCR spatial melting information about product. The speed integration of make conducive while-you-wait diagnostic tests do not require post-PCR analysis.

Zebrafish are a valuable model organism in biomedical research. Their rapid development, ability to human diseases, utility for testing genetic variants identified from next-generation sequencing, amenity CRISPR mutagenesis, and potential therapeutic compound screening, has led their wide-spread adoption diverse fields of study. However, power large-scale screens is limited by the absence automated genotyping tools live animals. This constrains drug screen options, limits analysis embryonic...

Immotile and rare sperm isolation from a complex cell background is an essential process for infertility treatment. The traditional collection biopsy sample requires long, tedious searches, yet still results in low retrieval. In this work, high recovery, throughput separation proposed the clinical retrieval process. It found that have different focusing positions compared with non-sperm cells inertial flow, which explained by alignment phenomenon. Separation spiral channel device 95.6%...

We present a new fabrication protocol for fabricating pneumatically controlled microvalve arrays (consisting of 100 s microvalves) in PDMS substrates. The utilizes rapid and cost-effective molds using laser cutting adhesive vinyl tapes replica molding PDMS. Hence the is fast, simple avoids cleanroom use. results show that effective doormat-style microvalves can be easily fabricated by manipulating stiffness actuating membrane through varying valve-chamber area/shape. Three frequently used...

Abstract Sperm preparation is critical to achieving a successful intrauterine insemination and requires the processing of semen sample remove white blood cells, wash away seminal plasma, reduce volume. We present an automated instrument capable performing sperm starting with diluted sample. compare our device against density gradient centrifugation by 0.5 mL portions patient samples through each treatment. In 5 min operating time, recovers average 86% all 82% progressively motile from...

Electroporation is regularly used to deliver agents into cells, including transgenic materials, but it not for mutating zebrafish embryos due the lack of suitable systems, information on appropriate operating parameters, and challenges posed by protective chorion. Here, a novel method gene delivery in was developed combining microinjection space between chorion embryo followed electroporation. This eliminates need removal injecting finding identifying key cell locations before performing an...

This paper reports fabrication of a magnetic resonance imaging (MRI) phantom created by stacking multiple thin polydimethylsiloxane (PDMS) layers. PDMS is spin coated on SU-8 molds to obtain the desired layer thickness and imprints microchannel patterns that define geometry. also identifies unique challenges related assembly layers for first time large number laminates this nature. Use photolithography techniques allows us create wide range geometries. The target dimensions phantoms reported...

We report a novel sperm trapping microfluidic chip that can trap and then transfer entrapped to individual collectors (cryotips) be detached from the chip. were able collect in numbers ranging 3–12 with good recovery of cryotip after transfer. Based on our knowledge, this is first time has been designed enable entrapment low cells while provisioning design subsequent observation those few off also discuss critical operational challenges need taken into consideration for deliver its outcome....

You have accessJournal of UrologyInfertility: Therapy I (PD34)1 Apr 2019PD34-07 A NOVEL MICROFLUIDIC DEVICE TO IMPROVE SPERM SEARCHING IN MICRODISSECTION TESE SAMPLES Alex Jafek*, Haidong Feng, Timothy Jenkins, Kenneth Aston, Douglas Carrell, Bruce Gale, Raheel Samuel, and Jim Hotaling Jafek*Alex Jafek* More articles by this author , FengHaidong Feng JenkinsTimothy Jenkins AstonKenneth Aston CarrellDouglas Carrell GaleBruce Gale SamuelRaheel Samuel HotalingJim View All Author...

Microfluidic devices for rapid and highly sensitive detection of living organisms were developed two applications. First, a zebrafish embryo genotyping system was shown to be able genotype embryos in the first 48 hours life without damaging any apparent way. Second, bacteria platform has been pathogens. The relies on magnetic bead extraction followed by secondary attachment. beads are barcoded with DNA sequences enriched Gs. guanine molecules generate an electrochemical response after they...

You have accessJournal of UrologyInfertility: Therapy II (MP52)1 Apr 2019MP52-05 RAPID MICROFLUIDIC SPERM PREPARATION FOR INTRAUTERINE INSEMINATION Alex Jafek*, Hayden Brady, Haidong Feng, Jim Hotaling, Douglas Carrell, Bruce Gale, Raheel Samuel, Kenneth Aston, and Timothy Jenkins Jafek*Alex Jafek* More articles by this author , BradyHayden Brady FengHaidong Feng HotalingJim Hotaling CarrellDouglas Carrell GaleBruce Gale SamuelRaheel Samuel AstonKenneth Aston JenkinsTimothy View All Author...