A5082604093- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Insect Pest Control Strategies
- CRISPR and Genetic Engineering
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Genetically Modified Organisms Research
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Silk-based biomaterials and applications
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Insect behavior and control techniques
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Bacterial biofilms and quorum sensing
- Fungal Plant Pathogen Control
- Genomics, phytochemicals, and oxidative stress
- Tendon Structure and Treatment
- Plant Disease Management Techniques
Institute of Vegetables and Flowers
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Sanya University
2024
Inner Mongolia University
2023-2024
Xi'an University of Science and Technology
2024
Shanghai Sixth People's Hospital
2023-2024
Shanghai Jiao Tong University
2023-2024
Lanzhou University
2023
Ministry of Agriculture and Rural Affairs
2022
Xi'an Jiaotong University
2020
Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led field-evolved resistance several lepidopteran Resistance Cry1Ac toxin diamondback moth, Plutella xylostella (L.), was previously mapped a multigenic locus (BtR-1). Here, we assembled 3.15 Mb BtR-1 and found high-level four independent P. strains were all associated with...
Significance Most studies of pesticide resistance have focused on the identification and functional analysis genes, but few considered signaling pathways involved in their regulation. In this work, we discovered that overexpression a P450 confers to neonicotinoid insecticides whitefly is trans -regulated by transcription factor CREB . Further demonstrated exposure activates key pathway cellular response extracellular signals, MAPK pathway, phosphorylation. then binds specific site promoter...
Understanding the molecular mechanisms underlying insect resistance to Bacillus thuringiensis (Bt) pesticidal proteins is crucial for sustainable pest management. Here, we found that downregulation of Plutella xylostella ecdysone oxidase gene (PxEO) in normal feeding stages contributes increased 20-hydroxyecdysone (20E) titer and mediates Bt Cry1Ac toxin. The PxEO was cloned its expression significantly downregulated midgut Bt-resistant Cry1Ac-selected P. xylostella. Silencing reduced...
Abstract BACKGROUND The whitefly Bemisia tabaci is a notorious agricultural pest known for its ability to cause significant crop damage through direct feeding and virus transmission. Its remarkable adaptability reproductive capacity are linked acquire integrate horizontally transferred genes (HTGs) into genome. These HTGs increase the physiological metabolic capacities of this pest, including cholesterol synthesis, which critical survival success. Among these genes, we identified...
The arms race between entomopathogenic bacteria and their insect hosts is an excellent model for decoding the intricate coevolutionary processes of host-pathogen interaction. Here, we demonstrate that MAPK signaling pathway a general switch to trans-regulate differential expression aminopeptidase N other midgut genes in host, diamondback moth (Plutella xylostella), thereby countering virulence effect Bacillus thuringiensis (Bt) toxins. Moreover, cascade activated fine-tuned by crosstalk two...
A cytochrome P450 that confers insecticide resistance in whitefly is regulated by an epitranscriptomic mechanism.
Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as hubs these complex interactions. We have previously shown that an insect hormone modulated cascade participates a general switch to trans -regulate differential expression diverse midgut genes in diamondback moth, Plutella xylostella (L.) cope with insecticidal action Cry1Ac toxin, produced by entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between...
Abstract Background Biopesticides and transgenic crops based on Bacillus thuringiensis (Bt) toxins are extensively used to control insect pests, but the rapid evolution of resistance seriously threatens their effectiveness. Bt is often polygenic complex. Mutations that confer occur in midgut proteins act as cell surface receptors for toxin, it thought they facilitate its assembly a membrane-damaging pore. However, mechanistic details action remain controversial. Results We have examined...
The benefits of biopesticides and transgenic crops based on the insecticidal Cry-toxins from Bacillus thuringiensis (Bt) are considerably threatened by insect resistance evolution, thus, deciphering molecular mechanisms underlying to Bt products is great significance their sustainable utilization. Previously, we have demonstrated that down-regulation PxmALP in a strain Plutella xylostella (L.) highly resistant Cry1Ac toxin was due hormone-activated MAPK signaling pathway contributed...
Abstract Maintaining fitness during pathogen infection is vital for host survival as an excessive response can be detrimental the itself. Fitness costs are frequently associated with insect hosts countering toxic effect of entomopathogenic bacterium Bacillus thuringiensis (Bt), which delay evolution resistance to this pathogen. The pest Plutella xylostella has evolved a mechanism resist Bt toxins without incurring significant costs. Here, we reveal that non-phosphorylated and phosphorylated...
Ongoing host-pathogen interactions can trigger a coevolutionary arms race, while genetic diversity within the host facilitate its adaptation to pathogens. Here, we used diamondback moth (
•Increased titer of the insect hormone 20-hydroxyecdysone (20E) facilitates an host, Plutella xylostella, to defeat its bacterial pathogen Bacillus thuringiensis (Bt).•Glucose dehydrogenase (GLD) was identified as a new ecdysone-degrading enzyme that can metabolize 20E.•A midgut miRNA initiated epigenetic regulatory pathway represses GLD activity and elevates 20E resist Bt pathogen.•An as-yet uncharacterized negative feedback loop reduces excess balance hormonal homeostasis.•This study...
Abstract The limitations of protein‐based hydrogels, including their insufficient mechanical properties and restricted biological functions, arise from the highly specific functions proteins as natural building blocks. A potential solution to overcome these shortcomings is development protein–protein which integrate structural functional proteins. In this study, a hydrogel formed by crosslinking bovine serum albumin (BSA) genetically engineered intrinsically disordered collagen‐like protein...
Abstract A recently emerging cell death pathway, known as copper‐induced death, has demonstrated significant potential for treating infections. Existing research suggests that cells utilizing aerobic respiration, opposed to those reliant on glycolysis, exhibit greater sensitivity death. Herein, a MnO 2 ‐loaded copper metal–organic frameworks platform is developed denoted MCM, enhance bacterial cuproptosis‐like via the remodeling of respiratory metabolism. The reversal hypoxic...
Plant viral diseases compromise the growth and yield of crop globally, they tend to be more serious under extreme temperatures drought climate changes. Currently, regulatory dynamics during plant development in response virus infection at cell level remain largely unknown. In this study, single-cell RNA sequencing on 23 226 individual cells from healthy tomato chlorosis virus-infected leaves was established. The specific expression epigenetic landscape each type stage were depicted. Notably,...
Abstract The recent discovery that various insects have acquired functional genes through horizontal gene transfer (HGT) has prompted numerous studies into this puzzling and fascinating phenomenon. So far, horizontally transferred are found to be functionally conserved largely retained their ancestral functions. It evidently not yet been considered may evolve can contribute divergence between species. Here, it is first showed the genomes of two widespread agriculturally important whiteflies...
Abstract Insect pests cause serious crop damage and develop high-level resistance to chemical insecticides Bacillus thuringiensis (Bt) insecticidal Cry toxins. A new promising approach for controlling them overcoming this is RNA interference (RNAi). The RNAi-based insect control strategy depends on the selection of suitable target genes. In study, we cloned characterized a novel ABC transporter gene PxABCH1 in diamondback moth, Plutella xylostella (L.). Phylogenetic analysis showed that...
The two-spotted spider mite Tetranychus urticate is an important agricultural pest worldwide. It extremely polyphagous and has developed resistance to many pesticides. Here, we assessed the pesticide of seven field populations T. urticae in China, their target site mutations activities detoxification enzymes. results showed that abamectin traditional pesticides pyridaben, profenofos bifenthrin had higher or lower toxicity than more recently including chlorfenapyr, spinetoram, cyflumetofen,...
Nitrogen is an essential element for all life on earth. metabolism, including excretion, growth, development, and survival of plants animals alike. Several nitrogen metabolic processes have been described, but the underlying molecular mechanisms are unclear. Here, we reveal a unique process metabolism in whitefly Bemisia tabaci , global pest. We show that it has acquired two bacterial uricolytic enzyme genes, B. urea carboxylase ( BtUCA ) allophanate hydrolase BtAtzF ), through horizontal...