A5026492212- Tardigrade Biology and Ecology
- Biocrusts and Microbial Ecology
- Spaceflight effects on biology
- Hemoglobin structure and function
- bioluminescence and chemiluminescence research
- Physiological and biochemical adaptations
- Mesenchymal stem cell research
- Cancer Research and Treatments
- Cell death mechanisms and regulation
- Insect and Arachnid Ecology and Behavior
- Microbial Inactivation Methods
- Tissue Engineering and Regenerative Medicine
- Mollusks and Parasites Studies
- Hepatitis B Virus Studies
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Protist diversity and phylogeny
- Bacteriophages and microbial interactions
- Hemiptera Insect Studies
- Magnetic and Electromagnetic Effects
Kazan Federal University
2014-2022
Abstract The larvae of the African midge, Polypedilum vanderplanki , can enter an ametabolic state called anhydrobiosis to overcome fatal desiccation stress. Pv11 cell line, derived from P. embryo, shows tolerance when treated with trehalose before and resumes proliferation after rehydration. However, molecular mechanisms this remain unknown. Here, we performed high-throughput CAGE-seq mRNA a differentially expressed gene analysis in trehalose-treated, desiccated, rehydrated cells, followed...
Desiccation-tolerant cultured cells Pv11 derived from the anhydrobiotic midge embryo endure complete desiccation in an ametabolic state and resume their metabolism after rehydration. These features led us to develop a novel dry preservation technology for enzymes as it was still unclear whether could preserve exogenous enzyme state. This study shows that protect desiccation-sensitive enzyme, luciferase (Luc), preserving enzymatic activity even storage 372 days at room temperature. A process...
Abstract Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature P. genome is presence expanded gene clusters associated with anhydrobiosis. While several such represent orthologues genes, there a distinct set genes . These include Lea-Island-Located (LIL) no except two LEA , PvLea1 and PvLea3 However, PvLIL proteins lack typical features as intrinsic...
Extracellular bacterial ribonucleases such as binase from Bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. Moreover, they may induce activation of tumor-derived macrophages either into the M1-phenotype well-documented functions in regulation antitumor immune response or M2-macrophages that stimulate growth, metastasis, and angiogenesis. In this study, endogenous RNase1 (but not RNA short oligonucleotides) stimulated expression...
Anhydrobiosis, an adaptive ability to withstand complete desiccation, in the nonbiting midge Polypedilum vanderplanki, is associated with emergence of new multimember gene families, including a group 27 genes late embryogenesis abundant (LEA) proteins (PvLea). To obtain insights into possible functional specialization these genes, we investigated expression and localization PvLea P. vanderplanki-derived cell line (Pv11), capable anhydrobiosis. We confirmed that all but two identified genome...
Abstract Anhydrobiosis, an adaptive ability to withstand complete desiccation, is in insects limited a single species: the nonbiting midge Polypedilum vanderplanki (the sleeping chironomid). Evolution of anhydrobiosis representative large genus associated with drastic changes genome structure, including emergence new multimember gene families directly involved desiccation tolerance. Among them, Late Embryogenesis Abundant (LEA) proteins, which protect other proteins from aggregation caused...
Abstract Desiccation-tolerant cultured cells Pv11 derived from the anhydrobiotic Polypedilum vanderplanki embryo endure complete desiccation because of their ametabolic state and resume metabolism after rehydration. These features led us to develop a novel dry preservation technology for enzymes as it was still unclear whether preserved an exogenous enzyme in state. This study shows that protect desiccation-sensitive enzyme, luciferase, preserving enzymatic activity even storage 372 days at...
ABSTRACT The larvae of the African midge, Polypedilum vanderplanki , can enter an ametabolic state called anhydrobiosis to conquer fatal desiccation stress. Pv11 cell line, derived from embryos shows tolerance by pretreatment with trehalose before desiccation; they resume proliferation after rehydration. To address underlying molecular mechanisms, we desiccated cells medium containing and induced We collected at each rehydration step performed CAGE-seq mRNA those cells. By analysing...