A5101685078- Marine and Coastal Ecosystems
- Marine and coastal plant biology
- Seaweed-derived Bioactive Compounds
- Extracellular vesicles in disease
- Fisheries and Aquaculture Studies
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Food Industry and Aquatic Biology
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Thyroid Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Marine Sponges and Natural Products
- Protein Hydrolysis and Bioactive Peptides
- Marine Bivalve and Aquaculture Studies
- 3D Printing in Biomedical Research
- Food Quality and Safety Studies
- Ecology and Conservation Studies
- RNA Interference and Gene Delivery
- Food and Agricultural Sciences
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Bone Metabolism and Diseases
Kyungpook National University
2015-2024
Swamy Vivekanadha Medical College Hospital and Research Institute
2023-2024
Institute of Medical Sciences
2022
Kyungpook National University Hospital
2014-2020
Pukyong National University
2013-2014
University of Madras
2011
Central Marine Fisheries Research Institute
1978-1986
Objective: Exosomes are nanovesicles that released from normal and tumor cells detectable in cell culture supernatant human biological fluids.Although previous studies have explored exosomes cancer cells, little is understood regarding the functions of by cells.Natural killer (NK) display rapid immunity to metastatic or hematological malignancies, efforts been undertaken clinically exploit antitumor properties NK cells.However, characteristics derived remain unknown.In this study, we...
Exosomes derived from mesenchymal stem cells (MSCs) have been evaluated for their potential to be used as drug delivery vehicles. Synthetically personalized exosome mimetics (EMs) could the alternative vesicles delivery. In this study, we aimed isolate EMs human MSCs. Cells were mixed with paclitaxel (PTX) and PTX-loaded (PTX-MSC-EMs) isolated anticancer effects against breast cancer. bone marrow-derived MSCs (4 × 106 cells/mL) or without PTX at different concentrations in phosphate-buffered...
Abstract Hair loss is a common medical problem. In this study, we investigated the proliferation, migration, and growth factor expression of human dermal papilla (DP) cells in presence or absence treatment with mesenchymal stem cell extracellular vesicles (MSC-EVs). addition, tested efficacy MSC-EV on hair an animal model. increased DP proliferation elevated levels Bcl-2, phosphorylated Akt ERK. addition; treated MSC-EVs displayed secretion VEGF IGF-1. Intradermal injection into C57BL/6 mice...
Inhibition of apoptosis leads to activation cell survival factors (e.g., AKT) causes continuous proliferation in cancer. Apoptosis, the major form cellular suicide, is central various physiological processes and maintenance homeostasis multicellular organisms. A number discoveries have clarified molecular mechanism apoptosis, thus clarifying link between factors, which has a therapeutic outcome. Induction inhibition by anticancer agents been shown correlate with tumor response. Cellular...
In vivo biodistribution and fate of extracellular vesicles (EVs) are still largely unknown require reliable in tracking techniques. this study, bioluminescence imaging (BLI) using Renilla luciferase (Rluc) was developed applied to monitoring EVs derived from thyroid cancer (CAL-62 cells) breast (MDA-MB-231) nude mice after intravenous administration compared with a dye-based labeling method for EV CAL-62 cells. The were successfully labeled Rluc visualized by BLI mice. distribution the EVs,...
Exosomes are endogenous nanocarriers that can deliver biological information between cells. They secreted by all cell types, including immune cells such as natural killer (NK) However, mammalian release low quantities of exosomes, and the purification exosomes is difficult. Here, nanovesicles were developed extrusion NK through filters with progressively smaller pore sizes to obtain exosome mimetics (NK-EM). The anti-tumour effect NK-EM was confirmed in vitro vivo. morphological features...
Exosomes are natural nano-sized membrane vesicles that have garnered recent interest owing to their potential as drug delivery vehicles. Though exosomes effective carriers, production and in vivo biodistribution still not completely elucidated. We analyzed the of exosome mimetics (EMs) from red blood cells (RBCs) radio-labeling RBC-EMs for imaging. Engineered EMs RBCs were produced large-scale by a one-step extrusion method, further purified density-gradient centrifugation. labeled with...
Objective Glioblastoma is a highly aggressive primary brain tumor that resistant to radiotherapy and chemotherapy. Natural killer (NK) cells have been used treat incurable cancers. Recent studies investigated the effectiveness of NK-cell-derived exosomes (NK-Exo) for treating cancers such as melanoma, leukemia, neuroblastoma; however, NK-Exo not glioblastoma. In present study, we antitumor effects against glioblastoma both in vitro vivo determined tumor-targeting ability by performing...
CXCR4, the stromal cell-derived factor-1 receptor, plays an important role in migration of hematopoietic progenitor/stem cells to injured and inflamed areas. Noninvasive cell tracking methods could be useful for monitoring fate. Therefore, this study, we evaluated efficacy intravenous infusion genetically engineered mesenchymal stem (MSCs) overexpressing CXC chemokine receptor 4 (CXCR4) home tumor, by optical imaging. We constructed a retroviral vector containing CXCR with dual reporter...
Abstract Mesenchymal stem cells (MSCs) can be used as a therapeutic armor for cancer. Extracellular vesicles (EVs) from MSCs have been evaluated anticancer effects. In vivo targeting of EVs to the tumor is an essential requirement successful therapy. Therefore, non-invasive methods monitoring in animal models are crucial developing EV-based cancer therapies. The present study develop bioluminescent using Renilla luciferase (Rluc)-expressing MSCs. MSC/Rluc (EV-MSC/Rluc) were visualized murine...
Mesenchymal stem cells (MSCs) show therapeutic effects in various types of diseases.MSCs have been shown to migrate towards inflamed or cancerous tissues, and visualized after sacrificing the animal.MSCs are able deliver drugs target cells, an ideal candidate for cancer therapy.The purpose this study was track migration MSCs tumor-bearing mice; were also used as drug delivery vehicles.Human breast (MDA-MB-231) anaplastic thyroid (CAL62) transduced with lentiviral particles, express Renilla...
Natural killer (NK) cells represent the third largest population of lymphocytes, and they play an important role in immune surveillance against tumors. The lungs are a common metastatic site for anaplastic thyroid cancer (ATC), metastasis is one most frequent causes mortality this type cancer. In current study, we evaluated effects NK cell-based immunotherapy pulmonary ATC determined how it affects effector molecules cells.Human (NK-92MI) were retrovirally transduced to express effluc gene....
Recent research suggests that advanced liver fibrosis could be reversed, but the therapeutic agents needed for prevention of remain to elucidated. The beneficial effects mesenchymal stem cells (MSCs) and MSC-derived extracellular vesicles (EVs) on have been reported. However, large-scale production MSC-EVs remains challenging. present study investigated mouse EV mimetics (MEVMs) in combination with curcumin (antifibrotic compound) using a model thioacetamide-induced fibrosis. MEVMs were...
Several clinical studies have demonstrated that increased macrophage infiltration into tumors confers metastatic potential and poor prognosis in cancer. Preclinical are needed to develop new strategies for countering metastasis. Our study was designed investigate the impact of pulmonary macrophages on lung metastasis anaplastic thyroid cancer (ATC). ATC (CAL-62) (Raw264.7) were transfected with effluc (CAL-62/effluc, Raw264.7/effluc). Coculture migration assays used assess effect Raw264.7 or...
Background: Radioactive iodine (RAI) therapy is an important strategy in the treatment of thyroid cancer. However, anaplastic cancer (ATC), a rare malignancy, exhibits severe dedifferentiation characteristics along with lack sodium iodide symporter (NIS) expression and function. Therefore, RAI ineffective contributes toward poor prognosis these patients. Recently, small-molecule tyrosine kinase inhibitors (TKIs) have been used to treat patients for restoring NIS function uptake capacity....
Radioactive-iodine (RAI) therapy is typically unprevailing as anaplastic thyroid cancer (ATC) management, owing to the decrease in endogenous sodium iodide symporter (NIS) expression. Therefore, new strategies for NIS re-induction are required improve efficacy of RAI ATC. In this study, we developed a novel high-throughput enhancer screening platform using dual reporter gene system identify potent tyrosine kinase inhibitor (TKI) and selected hit compound, K905-0266 TKI. The effects TKI...