A5100722138- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- Chronic Myeloid Leukemia Treatments
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Multiple Myeloma Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Synthesis and Reactions of Organic Compounds
- Cancer Research and Treatments
- Viral-associated cancers and disorders
- Wnt/β-catenin signaling in development and cancer
- CNS Lymphoma Diagnosis and Treatment
- Lymphatic System and Diseases
- Cancer Mechanisms and Therapy
Fujian Medical University
2015-2025
Southeast University
2021
New York Medical College
2010
Union Hospital
2005-2008
Hangzhou First People's Hospital
2006
Abstract Chronic lymphocytic leukemia/small lymphoma (CLL/SLL) has different epidemiology in Chinese vs. Western patients, but there are few studies of CLL/SLL large populations patients. ALPINE is a global phase 3 trial investigating Bruton tyrosine kinase inhibitors zanubrutinib ibrutinib to treat relapsed/refractory (R/R) CLL/SLL. Here we report results from the subgroup Adults with R/R were randomized 1:1 receive (160 mg twice-daily) or (420 once-daily) until disease progression...
Autoimmune haemolytic anaemia (AIHA) is common secondary to diffuse large B-cell lymphoma (DLBCL). However, there are no reports on tumour B-cells differentiating into plasmablasts in DLBCL AIHA. To state impact of the interferon regulatory factor 4 (IRF4) and explore mechanism IRF4 plasmablast differentiation.We analysed expression immunity-related genes from Gene Expression Omnibus correlated predictors clinical laboratory data using R package various statistical tools. Western blotting...
Phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway is a therapy cancer. We aimed to confirm the effect dual PI3K/mTOR inhibitor NVP-BEZ235 on proliferation, apoptosis, and autophagy chronic myelogenous leukemia (CML) cells sensitivity tyrosine kinase in vitro.Two human CML cell lines, K562 KBM7R (T315I mutant strain), were used. The proliferation was detected by MTS (Owen's reagent) assay. Cell cycle apoptosis assay examined flow cytometric analysis....
Phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway is a therapy cancer. We aimed to confirm the effect dual PI3K/mTOR inhibitor NVP-BEZ235 on cell proliferation and apoptosis in Burkitt lymphoma (BL) cells.Two human BL lines, CA46 RAJI were used this study. The cells was detected by manganese tricarbonyl transfer (MTT) assay. Cell cycle assay examined flow cytometric analysis. phosphorylation levels AKT (Thr308), (Ser473), RPS6 evaluated western blot...
Objective To determine whether an antimicrobial mouthrinse intervention reduces adverse pregnancy outcomes in a low‐resource rural area. Design Randomised controlled trial. Setting A county‐level maternal health care hospital China. Sample Pregnant women of less than 20 weeks gestation having periodontal disease. Methods Women with disease were randomised into the ( n = 232) and control group 234). Participants provided free (alcohol‐free containing 0.7% cetylpyridinium chloride) throughout...
Background Diffuse large B-cell lymphoma (DLBCL) is the most common in adults. CDGSH iron sulfur domain 2 (CISD2) an iron–sulfur protein and plays a critical role of cell proliferation. The aberrant expression CISD2 associated with progression multiple cancers. However, its DLBCL remains unclear. Methods differential was identified via public databases, quantitative real-time PCR (qRT-PCR) western blot were used to identifed CISD2. We estimated impact on clinical prognosis using Kaplan-Meier...
Arsenic Trioxide (ATO) has shown remarkable efficacy for the treatment of multiple myeloma (MM). However, mechanism by which ATO exerts its inhibitory effect on proliferation cells remains to be clarified. We study at various concentrations cell line RPMI 8226 and discussed molecular line. Our results proved that had a significant dose-dependent time-dependent expressions Notch receptor (Notch1) ligand (Jag2). Data from real-time PCR assay showed mRNA expression levels Jag2 gene downstream...
Abstract Diffuse large B-cell lymphoma (DLBCL) is the most common in adults. This study aimed to determine prognostic significance of endoplasmic reticulum (ER) stress-related genes DLBCL. ER were obtained from molecular signatures database. Gene expression data and clinical outcomes gene omnibus TCGA datasets collected, differentially expressed (DEGs) screened out. ontology enrichment analysis, kyoto encyclopaedia genomes pathway geneset analysis used analyse possible biological function...
Mantle cell lymphoma (MCL) is a rare and aggressive form of non-Hodgkin lymphoma. Challenges in its treatment include relapse, drug resistance, short survival period. The Hedgehog/GLI1 (Hh/GLI1) Wnt/β-catenin pathways are crucial cancer proliferation, survival, making them significant targets for anticancer research. This study aimed to assess the effectiveness combining inhibitors both against MCL investigate underlying molecular mechanisms. co-expression key proteins from Hh/GLI1 was...
In patients with untreated CD20-positive diffuse large B-cell lymphoma (DLBCL), a phase 3 trial was carried out to evaluate the efficacy and safety of zuberitamab plus CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone; Hi-CHOP) versus rituximab (R-CHOP) treatment regimens.
Background CDGSH iron-sulfur domain 2 (CISD2), an protein with a [2Fe-2S] cluster, plays pivotal role in the progression of various cancers, including Diffuse Large B-cell Lymphoma (DLBCL). However, mechanisms by which CISD2 regulates occurrence and development DLBCL remain to be fully elucidated. Methods The potential as predictive marker patients treated R-CHOP regimen was investigated through bioinformatics analysis clinical cohort studies. cell lines (SUDHL-4 HBL-1) were employed this...
To investigate the effects of BEZ235 on chronic myeloid leukemia (CML) cells. MTS assay was used to detect proliferation CML The proteins expression were detected by Western blot assay. autophagy in cells verified through transmission electron microscopy and evaluated laser confocal microscopy. Annexin V-FITC/PI double staining flow cytometry apoptosis. A xenograft model established observe therapeutic effect vivo. could inhibit cells; CQ 3-MA increase inhibition Z-VAD-FMK can reduce...
In this study, we investigated the role of calreticulin (CALR) in pathogenesis natural killer/T-cell lymphoma (NKTCL). CALR expression was significantly higher NKTCL tissues than normal control GSE80632 dataset. High correlated with poorer overall survival patients (P = 0.0248). mRNA and protein levels were cell lines (NK92, SNK6, SNT8) NK cells. CALR-silenced SNK6 cells generated smaller xenograft tumors immunodeficient NCG mice CALR-knockdown showed less vitro proliferation Transwell...
Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, effects mesenchymal on production patients with thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes cells; differentiation was observed by oil red O and Alizarin S staining; cell proliferation suppression measured MTS; levels cell-free supernatants were determined ELISA. inhibited activated CD4(+)T moreover, immunosuppressed soluble...
To investigate the effects of γ-secretase inhibitor (GSI), a Notch signalling inhibitor, on proliferation multiple myeloma cells in vitro and its molecular mechanism action.RPMI 8226 were treated with increasing concentrations GSI (0-20 µmol/l) for 24-72 h. Proliferation was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay. Cell-cycle analysis performed RPMI 0-10 µmol/l 48 h flow cytometry. Expression proteins (Notch1, Jagged 1 2), Bcl-2 phosphorylated...
BACKGROUND Dysregulation of the Hedgehog (Hh) pathway modulates various aspects hematologic and solid tumors, but its effects in human Natural killer/T-cell lymphoma (NKTCL) are unclear. Moreover, no study has examined consequences pharmacologically inhibiting Hh signaling NKTCL cell lines. MATERIAL AND METHODS In this study, expression Smoothened (Smo) Glioma-associated oncogene 1 (Gli1) tissue were scrutinized. Two lines, SNK6 SNT8, subjected to doses sonidegib (a Smo inhibitor) incubated...
To evaluate the association between vaginal microenvironment and fecundability among women.Register-based nationwide cohort study.Chinese National Free Pre-conception Check-up Project from 2015 to 2018.Our study included a total of 3 388 554 eligible women who were attempting become pregnant.We assessed at baseline by considering four indices: pH, clue cell examination, whiff test cleanliness grading. If any these indicators was abnormal, defined as poor. Propensity score matching used...
The aim of the present study was to explore possible mechanisms phosphatase and tensin homolog (PTEN) in pathogenesis Burkitt's lymphoma, provide novel information that can be used targeted treatment this disease. PTEN lentiviral overexpression vector short‑hairpin silencing vectors were constructed. effect on growth proliferation CA46 RAJI cells analyzed using a Cell Counting Kit‑8 assay. Apoptosis detected by Hoechst 33342 propidium iodide double staining. Flow cytometry analyze cell...
Chimeric antigen receptor (CAR) T cells target specific tumor antigens and lyse in an MHC-independent manner. However, the efficacy of CAR-T cell other cancer immunotherapies is limited by expression immune-checkpoint molecules such as programmed death-ligand 1 (PD-L1) on cells, which binds to PD-1 receptors leading inactivation immune escape. Here, we incorporated a PD-L1-targeted single-chain variable fragment (scFv) fusion protein sequence into CAR vector generate human anti-PD-L1-CAR-T...
Background Survivin is a rather specific gene in tumor tissue. We transfected dendritic cells (DCs) with recombinant adenovirus (Ad) containing survivin and granulocyte-macrophage colony-stimulating factor (GM-CSF) tested the inducing effect of DCs on cytotoxic T lymphocytes (CTL) to kill leukemic cells. Methods After derived from peripheral, was assayed by mixed leukocyte reaction (MLR) tests. Lactate dehydrogenase (LDH) release test used evaluate cytotoxicity CTL. Results Expression...
To explore the effects of mTOR inhibitor rapamycin on proliferation, cell cycle and apoptosis Burkitt's lymphoma line Raji CA46 cells its mechanism, so as to provide experimental evidence for a therapeutic target lymphoma.3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed assess inhibitory effect proliferation cells. The distribution analyzed by flow cytometry with propidium iodide(PI) single staining. FITC Annexin V+PI double expressions RPS6, p-RPS6,...
To investigate possible mechanism on protien LMP1 expressed by EBV inducing plasmablast differentiation of DLBCL cell via the mTORC1 pathway.