A5090931617- Hematopoietic Stem Cell Transplantation
- Acute Lymphoblastic Leukemia research
- Muscle Physiology and Disorders
- COVID-19 Clinical Research Studies
- Childhood Cancer Survivors' Quality of Life
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Neuroblastoma Research and Treatments
- Cardiomyopathy and Myosin Studies
- Chronic Lymphocytic Leukemia Research
- Antibiotic Resistance in Bacteria
- Blood disorders and treatments
- Hemoglobinopathies and Related Disorders
- Polyomavirus and related diseases
- CAR-T cell therapy research
- Pneumocystis jirovecii pneumonia detection and treatment
- Parvovirus B19 Infection Studies
- Autoimmune and Inflammatory Disorders Research
- Sarcoma Diagnosis and Treatment
- Histiocytic Disorders and Treatments
- COVID-19 and healthcare impacts
- Adipose Tissue and Metabolism
- Lymphoma Diagnosis and Treatment
Medanta The Medicity
2016-2025
Cancer Institute (WIA)
2018-2025
Medanta The Medicity
2017-2024
Englewood Hospital and Medical Center
2024
The Ohio State University
2013-2023
Fortis Memorial Research Institute
2016-2023
McGill University
2023
All India Institute of Medical Sciences Raipur
2015-2021
All India Institute of Medical Sciences
2014-2021
All India Institute of Medical Sciences Bhopal
2015-2021
Duchenne muscular dystrophy (DMD) is an inherited disease that causes striated muscle weakness. Recently, we showed therapeutic effects of the combination lisinopril (L), angiotensin converting enzyme (ACE) inhibitor, and spironolactone (S), aldosterone antagonist, in mice lacking dystrophin haploinsufficient for utrophin (utrn+/−;mdx, het mice); both cardiac skeletal function histology were improved when these treated early with LS. It was unknown to what extent LS treatment effective most...
Micro-dystrophin gene replacement therapies for Duchenne muscular dystrophy (DMD) are currently in clinical trials, but have not been thoroughly investigated their efficacy on cardiomyopathy progression to heart failure. We previously validated Fiona/dystrophin-utrophin-deficient (dko) mice as a DMD model that progresses reduced ejection fraction indicative of Adeno-associated viral (AAV) vector delivery an early generation micro-dystrophin prevented cardiac pathology and functional decline...
In this paper we describe an approach that combines stable isotope labeling of amino acids in cells culture, high mass accuracy liquid chromatography tandem spectrometry and a novel data analysis to accurately determine relative peptide post-translational modification levels. This describes the application discovery histone crosstalk networks Saccharomyces cerevisiae. Yeast mutants were generated mimic presence/absence 44 well-known modifications on core histones H2A, H2B, H3, H4. each...
The best-characterized acetylation of newly synthesized histone H4 is the diacetylation NH2-terminal tail on lysines 5 and 12. Despite its evolutionary conservation, this pattern modification has not been shown to be essential for either viability or chromatin assembly in any model organism. We demonstrate that mutations 12 yeast confer hypersensitivity replication stress DNA-damaging agents when combined with lysine 91, which also found a site soluble H4. In addition, these dramatic...
We describe here the outcomes of reduced-toxicity alternate-donor stem cell transplant (SCT) with posttransplant cyclophosphamide (PTCy) for primary immunodeficiency disorders (PIDs) in eight children (haploidentical-seven and matched unrelated donor-one). The conditioning was serotherapy (alemtuzumab-3/rabbit-anti-thymoglobulin-5); fludarabine, cyclophosphamide, total body irradiation-5 (additional thiotepa-3); fludarabine treosulfan-2; busulfan-1. All received PTCy 50 mg/kg on days 3 4 as...
Data on convalescent plasma therapy (CPT) in patients of hematological malignancies with severe Covid-19 is scarce.
Gene replacement for Duchenne muscular dystrophy (DMD) with micro-dystrophins has entered clinical trials, but efficacy in preventing heart failure is unknown. Although most patients DMD die from failure, cardiomyopathy undetectable until the teens, so trials young boys will be unknown a decade. Available animal models were sufficient to demonstrate micro-dystrophin on earlier onset skeletal muscle pathology underlying loss of ambulation and respiratory insufficiency patients. However, no...
BackgroundIn patients with end-stage heart failure, the primary etiology often originates in left ventricle, and eventually contractile function of right ventricle (RV) also becomes compromised. RV tissue-level deficits force and/or kinetics need quantification to understand involvement ischemic non-ischemic failing human myocardium.Methods resultsThe population suffering from failure is diverse, requiring many subjects be studied order perform an adequately powered statistical analysis....
An obligate anaerobic, mesophilic, motile and endospore-forming bacterium, designated 1D T , was isolated from a subsurface soil sample. The young culture of strain Gram-positive formed oval spores that were central in position. Based on the biochemical, chemotaxonomic physiological data, appears to be member genus Clostridium . Strain found capable degrading p -nitrophenol (pNP) at concentration 0.5 mM under anaerobic conditions as revealed by HPLC analysis. major fatty acids C 16 : 0...
The detailed epidemiological and molecular characterization of an outbreak Burkholderia cepacia at a neurotrauma intensive care unit level 1 trauma centre is described. stringent infection control interventions taken to successfully curb this are emphasized.The clinical microbiological data for those patients who had more than one blood culture that grew B. were reviewed. Bacterial identification antimicrobial susceptibility testing was done using automated Vitek 2 systems. Prospective...
The survival of children with cancer in India is inferior to that high-income countries. Indian Pediatric Hematology Oncology Group (IPHOG) held a series online meetings via www.Cure4kids.org identify barriers cure and develop strategies improve outcomes. Five major hurdles were identified: delayed diagnosis, abandonment, sepsis, lack co-operative groups, relapse. Development regional networks like IPHOG has allowed rapid identification local causes treatment failure for likely care outcomes...
Haploidentical family donor is universally available and fast emerging as an alternative choice for children with leukemia needing hematopoietic stem cell transplant (HSCT). Here we describe our experience of treating acute by haploidentical HSCT posttransplant cyclophosphamide (PTCy).We retrospectively analyzed the outcome data 17 who underwent related HSCT. Fifteen were in complete remission (CR) before HSCT: CR1-6, CR2-7, CR3-2 2 not remission. Donors mobilized granulocyte colony...
Allogenic hematopoietic cell transplantation (HCT) is the best curative approach for patients with severe aplastic anemia (SAA). The outcomes of HCT from haploidentical family donors (HFDs) have improved, making it a feasible option lacking an HLA-identical donor. However, data on HFD-HCT younger SAA sparse. In this multicenter retrospective study, we evaluated 79 undergoing SAA. All were heavily pretransfused, median time to was >12 months, and 67% had failed previous therapies....
Ochrobactrum anthropi is an unusual emerging pathogen especially in the hospital environment. Most of reported cases are nosocomially acquired infections patients with various indwelling and invasive medical devices, such as central venous catheters drainage tubes. We report a case transmitted catheter related septicaemia meningitis due to O. anthropi, elderly immunocompetent male head trauma admitted level -1 centre. This describes clinical microbiological characteristics rare also...
Leptospirosis is a zoonotic disease commonly affecting the tropical countries. It may have protean clinical manifestations including hepatorenal dysfunction, myocarditis, pulmonary haemorrhage, meningitis, optic neuritis and rhabdomyolysis. Neurological manifestation of leptospirosis without classical dysfunction rare entity. This complication can present with diverse central peripheral neurological presentations. The overlapping many common pathogens often pose diagnostic dilemma delay in...
Duchenne muscular dystrophy (DMD) is an X-linked inherited disease due to dystrophin deficiency causing skeletal and cardiac muscle dysfunction. Affected patients lose ambulation by age 12 usually die in the second third decades of life from respiratory failure. Symptomatic treatment includes use anti-inflammatory corticosteroids, which are associated with side effects including weight gain, osteoporosis, increased risk cardiovascular disease. Novel options include blockade...
Angiotensin converting enzyme inhibitors (ACEi) are the current standard of care treatment for cardiac dysfunction in Duchenne muscular dystrophy patients. We previously showed with an ACEi plus mineralocorticoid receptor (MR) antagonist improves limb and respiratory skeletal muscles, addition to a dystrophic mouse model at 20 weeks-of-age.To determine whether observed preclinical benefits MR on muscles can be reproduced by increasing dosage alone. also compared functional histological...