A5010623996- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Polysaccharides Composition and Applications
- Wheat and Barley Genetics and Pathology
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Studies on Chitinases and Chitosanases
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Carbohydrate Chemistry and Synthesis
- Transgenic Plants and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Bioenergy crop production and management
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Microbial Metabolic Engineering and Bioproduction
- Plant Stress Responses and Tolerance
- Enzyme Structure and Function
- Fermentation and Sensory Analysis
The University of Adelaide
2016-2025
Australian Wine Research Institute
2020-2024
Australian Research Council
2012-2022
ARC Centre of Excellence in Plant Energy Biology
2014-2020
Australian Centre for Plant Functional Genomics
2004-2017
University of Dundee
2016
James Hutton Institute
2016
The University of Melbourne
2006-2012
Plant Industry
1997-2012
ACT Government
2008-2012
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated ordered physical, genetic functional sequence resource that describes barley gene-space in structured whole-genome context. We developed physical map 4.98 Gb, more than 3.90 Gb anchored to high-resolution map. Projecting deep shotgun assembly, complementary DNA RNA data onto this framework supports...
Regulation de l'expression genique durant la germination caryopses cereales; contribution non seulement l'aleurone mais aussi du scutellum dans mobilisation l'albumen
Arabidopsis was transformed with double-stranded RNA interference (dsRNAi) constructs designed to silence three putative callose synthase genes: GLUCAN SYNTHASE–LIKE5 (GSL5), GSL6, and GSL11. Both wound papillary were absent in lines GSL5 dsRNAi a corresponding sequence-indexed T-DNA insertion line but unaffected GSL6 GSL11 lines. These data provide strong genetic evidence that the GSL genes of higher plants encode proteins are essential for formation. Deposition callosic plugs, or papillae,...
A characteristic feature of grasses and commercially important cereals is the presence (1,3;1,4)-beta-d-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-beta-d-glucan content barley grain cluster cellulose synthase-like CslF genes rice. After insertion rice into Arabidopsis, we detected walls transgenic plants using specific monoclonal antibodies enzymatic analysis. Because wild-type Arabidopsis does not contain or its...
Cell walls have been isolated from barley endosperm and found to contain a microfibrillar phase which is embedded in an amorphous matrix. The probably consists of cellulose, together with tightly bound arabinoxylan polysaccharides rich mannose. matrix material (approx. 25%) β-glucan 75%). Pectic are absent the cell walls. After successive removal water 1-M NaOH, only 6% wall remains. intra-cellular surfaces fragments extensively pitted, as result adpression starch granules into during...
Abstract A pan-transcriptome describes the transcriptional and post-transcriptional consequences of genome diversity from multiple individuals within a species. We developed barley using 20 inbred genotypes representing domesticated by generating analyzing short- long-read RNA-sequencing datasets tissues. To overcome single reference bias in transcript quantification, we constructed genotype-specific (RTDs) integrated these into linear pan-genome framework to create pan-RTD, allowing...
Sequence data from cDNA and genomic clones, coupled with analyses of expressed sequence tag databases, indicate that the CesA (cellulose synthase) gene family barley (Hordeum vulgare) has at least eight members, which are distributed across genome. Quantitative polymerase chain reaction been used to determine relative abundance mRNA transcripts for individual HvCesA genes in vegetative floral tissues, different stages development. To ensure accurate expression profiling, geometric averaging...
The walls of grasses and related members the Poales are characterized by presence polysaccharide ( 1 , 3 4 )-β-D-glucan (β-glucan). To date, only grass-specific cellulose synthase-like F CSLF ) gene family have been implicated in its synthesis. Assuming that other CSL genes also might encode synthases for this polysaccharide, we cloned HvCSLH1 a CSLH from barley Hordeum vulgare L.), expressed an epitope-tagged version cDNA Arabidopsis species with no β-glucan walls. Transgenic lines had...
Cell walls were isolated from the mesocarp of grape (Vitis vinifera L.) berries at developmental stages before veraison through to final ripe berry. Fluorescence and light microscopy intact revealed no measurable change in cell wall thickness as cells expanded ripening fruit. Isolated analyzed for their protein contents amino acid compositions, changes composition solubility constituent polysaccharides during development. Increases content after accompanied by an approximate 3-fold increase...
Summary Two mutant lines of barley, Risø 17 and Notch‐2, were found to accumulate phytoglycogen in the grain. Like sugary mutants maize rice, these phytoglycogen‐accumulating barley lack isoamylase activity developing endosperm. The shown be allelic, have lesions gene, isa1 that account for absence this enzyme. As well as causing a reduction endosperm starch content, mutations profound effect on structure, number timing initiation granules. There are no normal A‐type or B‐type granules...
Specific cDNA fragments corresponding to putative cellulose synthase genes (CesA) were inserted into potato virus X vectors for functional analysis in Nicotiana benthamiana by using virus-induced gene silencing. Plants infected with one group of cDNAs had much shorter internode lengths, small leaves, and a "dwarf" phenotype. Consistent loss cell wall cellulose, abnormally large many cases spherical cells ballooned from the undersurfaces particularly regions adjacent vascular tissues. Linkage...
Cellulose synthase-like CslF genes have been implicated in the biosynthesis of (1,3;1,4)-β-d-glucans, which are major cell wall constituents grasses and cereals. Seven from barley (Hordeum vulgare) can be divided into two classes on basis intron-exon arrangements. Four HvCslF mapped to a single locus chromosome 2H, region corresponding quantitative trait for grain (1,3;1,4)-β-d-glucan content. The other map chromosomes 1H, 5H, 7H, cases close loci Spatial temporal patterns transcription...
A major part of the daily caloric intake human societies around world is derived from a diverse range foods prepared members grass family, including wheat ( Triticum aestivum ), rice Oryza sativa sorghum Sorghum bicolor millets Panicum miliaceum and Pennisetum
Cell walls in commercially important cereals and grasses are characterized by the presence of (1,3;1,4)-β-d-glucans. These polysaccharides beneficial constituents human diets, where they can reduce risk hypercholesterolemia, type II diabetes, obesity colorectal cancer. The biosynthesis cell wall (1,3;1,4)-β-d-glucans Poaceae is mediated, part at least, cellulose synthase-like CslF family genes. Over-expression barley CslF6 gene under control an endosperm-specific oat globulin promoter...
The three-dimensional structures of (1-->3)-beta-glucanase (EC 3.2.1.39) isoenzyme GII and (1-->3,1-->4)-beta-glucanase 3.2.1.73) EII from barley have been determined by x-ray crystallography at 2.2- to 2.3-A resolution. two classes polysaccharide endohydrolase differ in their substrate specificity function. Thus, the (1-->3)-beta-glucanases, which are classified amongst plant "pathogenesis-related proteins," can hydrolyze (1-->3)- (1-->3,1-->6)-beta-glucans fungal cell...
Abstract Boron (B) is an essential micronutrient that affects plant growth at either deficient or toxic concentrations in soil. The aim of this work was to investigate the adaptation barley (Hordeum vulgare) plants B levels and increase our understanding toxicity tolerance mechanisms. We used a metabolomics approach compare metabolite profiles root leaf tissues intolerant, commercial cultivar (cv Clipper) B-tolerant Algerian landrace Sahara). After exposure elevated (200 1,000 μ m), number...