A5018862389- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Solid-state spectroscopy and crystallography
- Dialysis and Renal Disease Management
- bioluminescence and chemiluminescence research
- Physics of Superconductivity and Magnetism
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Photoreceptor and optogenetics research
- Immunodeficiency and Autoimmune Disorders
- Superconducting Materials and Applications
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Analytical Chemistry and Chromatography
- Neuroscience of respiration and sleep
- Urban and spatial planning
- Atomic and Subatomic Physics Research
- Photochromic and Fluorescence Chemistry
- NMR spectroscopy and applications
- Molecular spectroscopy and chirality
- European history and politics
RIKEN Center for Biosystems Dynamics Research
2021-2025
Osaka University
1995-2024
Protein Research Foundation
1995-2024
JEOL (Japan)
1989-2024
RIKEN
2004-2022
National Institute of Infectious Diseases
1999-2021
RIKEN Center for Integrative Medical Sciences
2004-2021
SPring-8
2019-2021
Yokohama City University
2008-2021
NHK Spring (Japan)
2019-2021
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Suite of Triple Resonance NMR Experiments for the Backbone Assignment 15N, 13C, 2H Labeled Proteins with High SensitivityToshio Yamazaki, Weontae Lee, Cheryl H. Arrowsmith, D. R. Muhandiram, and Lewis E. KayCite this: J. Am. Chem. Soc. 1994, 116, 26, 11655–11666Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTwo-dimensional NMR experiments for correlating carbon-13.beta. and proton.delta./.epsilon. chemical shifts of aromatic residues in 13C-labeled proteins via scalar couplingsToshio Yamazaki, Julie D. Forman-Kay, Lewis E. KayCite this: J. Am. Chem. Soc. 1993, 115, 23, 11054–11055Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMeasurement of 2H T1 and T1.rho. Relaxation Times in Uniformly 13C-Labeled Fractionally 2H-Labeled Proteins SolutionD. R. Muhandiram, Toshio Yamazaki, Brian D. Sykes, Lewis E. KayCite this: J. Am. Chem. Soc. 1995, 117, 46, 11536–11544Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/ja00151a018https://doi.org/10.1021/ja00151a018research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSegmental Isotope Labeling for Protein NMR Using Peptide SplicingToshio Yamazaki, Takanori Otomo, Natsuko Oda, Yoshimasa Kyogoku, Koichi Uegaki, Nobutoshi Ito, Yoshizumi Ishino, and Haruki NakamuraView Author Information Institute Research, Osaka University 3-2 Yamadaoka, Suita, 565-0871, Japan Biomolecular Engineering Research 6-2-3 Furuedai, 565-0874, National AIST 1-8-31 Midorigaoka, Ikeda, 563-8577, Cite this: J. Am. Chem. Soc. 1998, 120,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNMR Experiments for the Measurement of Carbon Relaxation Properties in Highly Enriched, Uniformly 13C,15N-Labeled Proteins: Application to 13C.alpha. CarbonsToshio Yamazaki, Ranjith Muhandiram, and Lewis E. KayCite this: J. Am. Chem. Soc. 1994, 116, 18, 8266–8278Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September...
The structure of the carboxyl-terminal domain Escherichia coli RNA polymerase α subunit (αCTD), which is regarded as contact site for transcription activator proteins and promoter UP element, was determined by nuclear magnetic resonance spectroscopy. Its compact four helices two long arms enclosing its hydrophobic core shows a folding topology distinct from those other DNA-binding proteins. element binding found on surface comprising helix 1, amino-terminal end 4, preceding loop. Mutation...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn HNCA Pulse Scheme for the Backbone Assignment of 15N,13C,2H-Labeled Proteins: Application to a 37-kDa Trp Repressor-DNA ComplexToshio Yamazaki, Weontae Lee, Matthew Revington, Debra L. Mattiello, Frederick W. Dahlquist, Cheryl H. Arrowsmith, and Lewis E. KayCite this: J. Am. Chem. Soc. 1994, 116, 14, 6464–6465Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July...
201TI was used as an imaging agent in 173 malignant tumors and 76 benign lesions. The sensitivity, specificity, accuracy were 0.64, 0.61, 0.63, respectively. Sensitivity good thyroid cancer (0.91) fair primary lung (0.70) liver (0.71). Compared with 67Ga, appears to have a higher sensitivity nearly the same cancer. might be useful distinguishing cold nodules differentiating from metastases.
Mammalian Musashi1 (Msi1) is an RNA-binding protein that regulates the translation of target mRNAs, and participates in maintenance cell 'stemness' tumorigenesis. Msi1 reportedly binds to 3'-untranslated region mRNA Numb, which encodes Notch inhibitor, impedes initiation its by competing with eIF4G for PABP binding, resulting triggering signaling. Here, mechanism recognizes RNA sequence using Ribonucleoprotein (RNP)-type domains (RBDs), RBD1 RBD2 has been revealed on identification minimal...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRearrangements and ring expansions during the deoxygenation of .beta.,.beta.-disubstituted o-nitrostyrenesRichard J. Sundberg Toshio YamazakiCite this: Org. Chem. 1967, 32, 2, 290–294Publication Date (Print):February 1, 1967Publication History Published online1 May 2002Published inissue 1 February 1967https://pubs.acs.org/doi/10.1021/jo01288a009https://doi.org/10.1021/jo01288a009research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract Understanding hydrogen-bonding networks in nanocrystals and microcrystals that are too small for X-ray diffractometry is a challenge. Although electron diffraction (ED) or 3D crystallography applicable to determining the structures of such owing their strong scattering power, these techniques still lead ambiguities hydrogen atom positions misassignments atoms with similar atomic numbers as carbon, nitrogen, oxygen. Here, we propose technique combining ED, solid-state NMR (SSNMR),...
NMR studies can provide unique information about protein conformations in solution. In CASP14, three reference structures provided by solution methods were available (T1027, T1029, and T1055), as well a fourth data set of NMR-derived contacts for an integral membrane (T1088). For the targets with NMR-based structures, best prediction results ranged from very good (GDT_TS = 0.90, T1055) to poor 0.47, T1029). We explored basis these comparing all CASP14 models against experimental data. T1027,...
Peptide segments in a protein, which can include an active site of interest or be series parts constituting the entire structure, are now selectively observed by nuclear magnetic resonance (NMR) spectroscopy using samples prepared intein-mediated ligation method. Two separate inteins were used to ligate NMR-transparent both ends NMR-visible segment, producing partly visible intact protein molecule. The 15N−1H correlation spectrum 370-residue maltose binding labeled with 15N at continuous...
Macrophages (Mphis) have various functions and play a critical role in host defense the maintenance of homeostasis. Mphis exist every tissue body, but from different tissues exhibit wide range phenotypes with regard to their morphology, cell surface antigen expression function, are called by names. However, precise mechanism generation macrophage heterogeneity is not known. In present study, authors examined functional generated human monocytes under influence granulocyte-macrophage...
Abstract Gaussia luciferase (GLuc) is a small (18.2 kDa; 168 residues) and thus attracting much attention as reporter protein, but the lack of structural information hampering further application. Here, we report first solution structure fully active, recombinant GLuc determined by heteronuclear multidimensional NMR. We obtained natively folded bacterial expression efficient refolding using Solubility Enhancement Petide (SEP) tag. Almost perfect assignments GLuc’s 1 H, 13 C 15 N backbone...
Amyloid-β (Aβ) fibrils in neuritic plaques are a hallmark of Alzheimer's disease (AD). Since the 42-residue Aβ (Aβ42) fibril is most pathogenic among different species, its structural characterization crucial to our understanding AD. While several polymorphs have been reported for Aβ40, previous studies Aβ42 prepared at neutral pH detected essentially only one structure, with an S-shaped β-sheet arrangement (e.g., Xiao et al. Nat. Struct. Mol. Biol. 2015, 22, 499). Herein, we demonstrate...
Background Buruli ulcer (BU) caused by Mycobacterium ulcerans (M. ulcerans) has emerged as an important public health problem in several rural communities sub-Saharan Africa. Early diagnosis and prompt treatment are preventing disfiguring complications associated with late stages of the disease progression. Presently there is no simple rapid test that appropriate for early use low-resource settings where M. most prevalent. Methodology We compared conventional pocket warmer loop mediated...